We narrowed to 7,039 results for: SIM

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pLIB-Strep(II)(C)-CTR9

Plasmid

#231725

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CTR9 (CTR9 Human)

Tags

3C-Strep(II)

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAcGP67A-mMD2-proA

Plasmid

#187878

Purpose

baculovirus protein expression of mouse MD-2 (residues 19–160) fused to a protein A tag

Depositor

Bruce Beutler

Article

Wang et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

Insert

MD2 (Ly96 Mouse)

Expression

Insect

Mutation

includes residues 19–160 of mouse MD-2

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLIB-FLAG-GST(N)-RPB1-CTD(1586-1971)

Plasmid

#231721

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

POLR2A (POLR2A Human)

Tags

Flag-GST-3C

Expression

Insect

Mutation

RPB1:1586-1971

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAcBac1-EcTrp-TGA

Plasmid

#228780

Purpose

aaRS/tRNA system for incorporation of 5HTP within proteins in mammalian cells at TGA codon

Depositor

Abhishek Chatterjee

Article

Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

Inserts

EcTrpRS
EcWtR

Use

Baculoviral

Expression

Mammalian

Mutation

TGA suppressor

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET-45b-HOXD13-IDR-mCherry

Plasmid

#145280

Purpose

Bacterial Expression of 6xHis HOXD13 IDR mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13-IDR (HOXD13 Human)

Expression

Bacterial

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 FLAG FBXO31

Plasmid

#236429

Purpose

transient overexpression of FBXO31 in mammalian cells

Depositor

Michele Pagano

Article

Duan et al Cell Rep. 2021 Oct 19;37(3):109870. doi:

Insert

FBXO31 (FBXO31 Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-Halo-hMRE11(wt)

Plasmid

#208393

Purpose

To generate stable cell line expressing Halo-human MRE11(WT) by Retroviral infection.

Depositor

Gaorav Gupta

Article

Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

Insert

Human MRE11 (MRE11 Human)

Use

Luciferase and Retroviral

Expression

Mammalian

Mutation

H129N

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-BE3-P2A-GFP-PGK-Puro

Plasmid

#110867

Purpose

Lentiviral vector for constitutive expression of BE3-P2A-GFP (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3

Use

Lentiviral

Mutation

D10A

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pRRL.SIN.EF1A.JAG1-B5.GS.CAR-3G

Plasmid

#194464

Purpose

CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.B5 in VH-VL order & (GGGGS)3 linker (scFv). GFP-Zeo for selection & monitoring.

Depositor

Ana Sofia Coroadinha

Article

Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

Insert

Anti-JAG1 CAR

Use

Lentiviral

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAcBac1-EcTyr-TAG

Plasmid

#228782

Purpose

EcTyr aaRS/tRNA system for mammalian ncAA incorporation

Depositor

Abhishek Chatterjee

Article

Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

Inserts

EcTyrRS
EcYtR
BstR

Use

Baculoviral

Expression

Mammalian

Mutation

TAG suppressor

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-PP-human-CHMP3

Plasmid

#154180

Purpose

expresses human CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3 (CHMP3 Human)

Tags

FLAG and Strep-Tag II

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDsRed-importinα

Plasmid

#119679

Purpose

Expresses DsRed-tagged human importinα in mammalian cells

Depositor

Kenji Sugimoto

Article

Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

Insert

importinα (KPNA2 Human)

Tags

DsRed

Expression

Mammalian

Mutation

Contains amino acids 251-529

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pMEL12

Plasmid

#107918

Purpose

hph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pRSFDUET-1_VNp-anti-Lysozyme fAb-BiFC

Plasmid

#214950

Purpose

Bacterial expression of anti-chicken lysozyme Fab light and heavy chain VNp fusions. Each chain is fused to separate halves of a BiFC protein -Venus fluorescence signifies Hc/Lc heterodimer formation.

Depositor

Dan Mulvihill

Article

Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

Inserts

VNp-anti-Lysozyme Fab heavy chain amino-BiFC fusion
VNp-anti-Lysozyme Fab light chain amino-BiFC fusion

Tags

6xHis, Amino half of venus BIFC fragment, Carboxy…

Expression

Bacterial

Promoter

T7

Available Since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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R711-X04-566: His6-MBP-tev-Hs.NF1iso2 (1198-1530)

Plasmid

#159579

Purpose

E. coli protein expression of His6-MBP-tev-Hs.NF1 DEF (1198-1530)

Depositor

Dominic Esposito

Article

Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

Insert

His6-MBP-tev-Hs.NF1 DEF (1198-1530) (NF1 Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-neo-PP1-Alpha-H248K

Plasmid

#171268

Purpose

Mammalian expression of 3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant that loses enzymatic activity

Depositor

Steven Balk

Article

Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

Insert

3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant (PPP1CA Human)

Tags

Flag

Expression

Mammalian

Mutation

PPP1CA-His248Lys (H248K)

Promoter

CMV

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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FokI-mCherry-LacI-NLS

Plasmid

#215096

Purpose

Expresses FokI-mCherry-LacI-NLS in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

FokI-mCherry-LacI-NLS

Expression

Mammalian

Promoter

CMV

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pT2-hUbC-dCas9-KRAB-T2a-Puro

Plasmid

#225999

Purpose

To establish stable dCas9-KRAB knock-in line

Depositor

Miguel Ramalho-Santos

Article

Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

Insert

humanized dCas9-KRAB T2A Puro

Use

Sleeping beauty transposon

Expression

Mammalian

Mutation

D10A and H840A

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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R711-X12-566: His6-MBP-tev-Hs.NF1iso2 (1203-1530)

Plasmid

#159580

Purpose

E. coli protein expression of His6-MBP-tev-Hs.NF1iso2 (1203-1530)

Depositor

Dominic Esposito

Article

Yan et al Cell Rep. 2020 Jul 21;32(3):107909. doi:

Insert

His6-MBP-tev-Hs.NF1iso2 (1203-1530) (NF1 Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-xCas9RA-P2A-Puro

Plasmid

#110871

Purpose

Lentiviral vector for constitutive expression of xCas9(3.7)RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xCas9(3.7)RA

Use

Lentiviral

Tags

FLAG

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-RUNX2(+10A)-IDR-mCherry-CRY2-NLS

Plasmid

#145268

Purpose

Mammalian Expression of Nucelar Opto RUNX2(+10A) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

RUNX2(+10A)-IDR (RUNX2 Human)

Expression

Mammalian

Mutation

A-repeat expanded

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-2X-P2A-Puro

Plasmid

#110840

Purpose

Lentiviral vector for constitutive expression of 2X in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-2X

Use

Lentiviral

Mutation

D10A and 2 tandem NLS sequences in the XTEN linker

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pRS0045

Plasmid

#131124

Purpose

Mammalian Target-ACE expression vector

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

TadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI

Use

CRISPR

Tags

PmCDA1, TadA, and UGI

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V …

Promoter

CMV promoter

Available Since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pFB-GST-IRP2

Plasmid

#226621

Purpose

Expresses human IRP2 in insect cells

Depositor

Ning Zheng

Article

Wang et al Mol Cell. 2020 Apr 2;78(1):31-41.e5. doi

Insert

IRP2 (IREB2 Human)

Tags

GST

Expression

Insect

Promoter

Polyhedrin

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-FNLS-PGK-Puro

Plasmid

#110844

Purpose

Lentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA-FNLS

Use

Lentiviral

Tags

3X FLAG

Mutation

D10A and NLS sequence at the N-terminus

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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OMM-short-RspA-NFAST

Plasmid

#233603

Purpose

Expression of RspA-NFAST on the outer mitochondrial membrane

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

RspA-NFAST

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-FLEX-ArchT-GFP

Plasmid

#58851

Purpose

AAV-mediated expression of ArchT-GFP under the EF1a promoter, in floxed/reversed (Cre-dependent) manner

Depositor

Edward Boyden

Article

Han et al Front Syst Neurosci. 2011;5:18. Epub 201

Insert

ArchT-GFP

Use

AAV and Cre/Lox

Tags

GFP

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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TurboID-13x-EV

Plasmid

#215086

Purpose

Expresses TurboID-13x[linker] in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

TurboID (birA )

Use

Retroviral

Tags

13x [linker]

Expression

Mammalian

Promoter

LTR

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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pMEL16

Plasmid

#107922

Purpose

HIS3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Article

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y

Use

CRISPR

Expression

Yeast

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pGFP-PTEN

Plasmid

#50519

Purpose

This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

Depositor

Kenneth Yamada

Article

Tamura et al Science. 1998 Jun 5;280(5369):1614-7.

Insert

PTEN (PTEN Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only