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mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax

Plasmid

#173870

Purpose

Mammalian expression of mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax (opto-RhoA)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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peGFP C1-SPG20 SC domain

Plasmid

#218580

Purpose

Expresses GFP-tagged SC domain of human SPG20 in mammalian cells

Depositor

Hemmo Meyer

Article

Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

Insert

SPG20 (SPART Human)

Tags

eGFP

Expression

Mammalian

Mutation

deleted amino acids 1-417

Promoter

CMV

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-VSVG

Plasmid

#80606

Purpose

Mammalian expression vector that expresses a VSV-G protein with a FLAG tag near the N-terminus of the protein

Depositor

Sven Eyckerman

Article

Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

Insert

VSV-G

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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CLC-EGFP

Plasmid

#193566

Purpose

Expresses human Clathrin Light Chain B tagged with mEGFP in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

Clathrin light chain B (CLTB Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

Plasmid

#173872

Purpose

Mammalian expression of mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax (opto-Cdc42)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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EF1a_MYCL_P2A_Hygro_Barcode

Plasmid

#120462

Purpose

Barcoded lentiviral vector to express MYCL in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYCL (MYCL Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir144[3072 nt]~451-CMV2-mir1

Plasmid

#164682

Purpose

Express miR-144[3072 nt]~451 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

miR-144[3072 nt]~451 (MIR144 Human)
mir-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-His:PYL1:nls:VPR

Plasmid

#135988

Purpose

Expresses the PYL1 abscisic acid-inducible domain fused to the VPR transcriptional activation domain in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

PYL1 domain fused to the VPR activation domain

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTS113 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-Alfa-P2A-TagBFP

Plasmid

#199366

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-ALFA-P2A-BFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

3C-SUMOEu-ALFA-P2A-TagBFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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EF1a_MYOD1_P2A_Hygro_Barcode

Plasmid

#120464

Purpose

Barcoded lentiviral vector to express MYOD1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYOD1 (MYOD1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-B/c

Plasmid

#227962

Purpose

Entry plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

B/c

Promoter

None

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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ppBAalbNixE1E2 PUb-GFP

Plasmid

#173667

Purpose

transgenesis vector for Aedes albopictus ectopic expression of the Nix gene

Depositor

Eric Marois

Article

Lutrat et al Commun Biol. 2022 Mar 7;5(1):210. doi: 1

Inserts

Nix masculinisation gene
EGFP

Expression

Insect

Mutation

Nix isoforms with only exons 1, 2 separated by in…

Promoter

Aedes aegypti Polyubiquitin and cloned Nix promot…

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-NES-SspB-SOS2cat-P2A-iLIDcaax

Plasmid

#173873

Purpose

Mammalian expression of mCherry-NES-SspB-SOS2cat-P2A-iLIDcaax (opto-Ras)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-SOS2cat-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-NES-SspB-MCS-P2A-iLIDcaax

Plasmid

#173869

Purpose

Mammalian expression of mCherry-NES-SspB-P2A-iLIDcaax (opto-control) containing multiple cloning sites (MCS)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mCherry-NES-SspB-MCS-P2A-iLIDcaax

Tags

C-terminal CAAX-box and mCherry

Expression

Mammalian

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-TWIN-STREP-FLAG-NPRL2

Plasmid

#164985

Purpose

NPRL2 expressing vector for mammalian cells

Depositor

Roberto Zoncu

Article

Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

Insert

NPRL2 (NPRL2 Human)

Tags

2xSTREP FLAG

Expression

Mammalian

Promoter

CAG

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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LITE2.0 EF1a_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_hGHpA

Plasmid

#47457

Purpose

LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. EF1-alpha promoter for ubiquitous mammalian expression.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

NLS(alpha-imp)-CRY2PHR-NLS-VP64

Tags

2A_GFP

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only

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GFP-WT (AF41)

Plasmid

#21367

Depositor

Gregory Germino

Article

Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

wild-type sequence*

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-HseIF4AI_AC

Plasmid

#148438

Purpose

Mammalian Expression of HseIF4AI

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HseIF4AI (EIF4A1 Human)

Expression

Mammalian

Mutation

3 silent mutations relative to NM_001416.2

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLXSN-EGFR

Plasmid

#65226

Purpose

Expression of EGFR in mammalian cells

Depositor

Axel Ullrich

Article

Obermeier et al EMBO J. 1996 Jan 2;15(1):73-82.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-MS2-HA-HsRck_AC

Plasmid

#148417

Purpose

Mammalian Expression of HsRck

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck (DDX6 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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