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We narrowed to 1,286 results for: MS2

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Showing: 681 - 720 of 1286 results

  1. lentiMPH v2

    Plasmid
    #89308
    Purpose
    lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker (EF1a-MS2-p65-HSF1-2A-Hygro-WPRE). This version has a higher virus titer.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Insert
    MS2-P65-HSF1_2A_Hygro (RELA Human, Synthetic)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1a
    Available Since
    March 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    89308-plasmid-map-sequence-id-425605

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  4. pPLP1H-mCf

    Plasmid
    #229984
    Purpose
    LIC vector for polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.
    Depositor
    Barry Lutz
    Article
    Kline et al Biotechnol Biotechnol Equip. 2025;39(1):
    Type
    Empty backbone
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229984-plasmid-map-sequence-id-458288

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  5. pPLP2H-mCf

    Plasmid
    #229985
    Purpose
    LIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.
    Depositor
    Barry Lutz
    Article
    Kline et al Biotechnol Biotechnol Equip. 2025;39(1):
    Type
    Empty backbone
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229985-plasmid-map-sequence-id-458287

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  6. pPLP3H-mCf

    Plasmid
    #229987
    Purpose
    LIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag for IMAC purification.
    Depositor
    Barry Lutz
    Article
    Kline et al Biotechnol Biotechnol Equip. 2025;39(1):
    Type
    Empty backbone
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229987-plasmid-map-sequence-id-458283

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  9. pPLP4HS-mCf

    Plasmid
    #229988
    Purpose
    LIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag and Twin-strep MP tag for AC purification.
    Depositor
    Barry Lutz
    Article
    Kline et al Biotechnol Biotechnol Equip. 2025;39(1):
    Type
    Empty backbone
    Tags
    His6 and Twin-Strep-tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229988-plasmid-map-sequence-id-458286

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  10. pPLP2S-mCf

    Plasmid
    #229986
    Purpose
    LIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. Strep-tag II CP tag for Strep-Tactin AC purification.
    Depositor
    Barry Lutz
    Article
    Kline et al Biotechnol Biotechnol Equip. 2025;39(1):
    Type
    Empty backbone
    Tags
    Strep-tag II
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    229986-plasmid-map-sequence-id-458306

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  11. lentiMPH v2 (Neo)

    Plasmid
    #92065
    Purpose
    Modified version of lentiMPH v2, a lenti vector encoding the MS2-P65-HSF1 activator helper complex and neo resistance marker (EF1a-MS2-p65-HSF1-2A-Neo-WPRE).
    Depositor
    Adam Karpf
    Article
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Insert
    MS2-P65-HSF1_2A_Neo (HSF1 Human, Synthetic)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    N55K in MS2
    Promoter
    EF1a
    Available Since
    June 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    92065-plasmid-map-sequence-id-181210

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  12. pA.CBh.TP-V5H6.MCh.Puro

    Plasmid
    #209950
    Purpose
    In mammalian cells expresses TP with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.
    Depositor
    Kevin Weeks
    Article
    Hatfield et al Biochemistry. 2025 Sep 16;64(18):3854-38
    Inserts
    TP (MS2 coat protein)
    mCherry fluorescent protein fused to puromycin resistance marker
    Tags
    V5 epitope with H6 tag
    Expression
    Mammalian
    Promoter
    CBh and CMV
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    209950-plasmid-map-sequence-id-418743

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  13. PB-SAM

    Plasmid
    #102559
    Purpose
    Piggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.
    Depositor
    Ying Liu
    Article
    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64
    Inserts
    MS2-P65-HSF1_T2A_Hygro
    dCAS9(D10A, N863A)-VP64_T2A_Blast
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9 and N55K in MS2
    Promoter
    CAG and EF1A
    Available Since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    102559-plasmid-map-sequence-id-193885

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  14. phage - ubc - nls - 2xmcp - mCherry - BirA*

    Plasmid
    #131136
    Purpose
    BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and mCherry; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.
    Depositor
    Ralf Jansen
    Insert
    NLS-HA-2xMCP-mCherry-BirA*
    Use
    Lentiviral
    Tags
    NLS, HA
    Expression
    Mammalian
    Mutation
    BirA* = BIrA >R118G
    Promoter
    human ubiquitin C (UBC)
    Available Since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131136-plasmid-map-sequence-id-255502

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  15. phage - ubc - nls - mcp - egfp - BirA*

    Plasmid
    #131134
    Purpose
    BirA* expressed as a protein fusion with MS2 coat protein (MCP) monomer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.
    Depositor
    Ralf Jansen
    Insert
    NLS-HA-MCP-EGFP-BirA*
    Use
    Lentiviral
    Tags
    NLS, HA
    Expression
    Mammalian
    Mutation
    BirA* = BIrA >R118G
    Promoter
    human ubiquitin C (UBC)
    Available Since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131134-plasmid-map-sequence-id-255499

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  16. pUBC_stdMCP_serinemod_E488QADAR_p2A_yGFP

    Plasmid
    #154787
    Purpose
    Expresses synonymized MS2 coat protein dimer (MCP) fused to hyperactive ADAR catalytic domain
    Depositor
    Robert Singer
    Article
    Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:
    Insert
    Adenosine deaminse (Adar Fly, Synthetic)
    Use
    Lentiviral
    Tags
    GFP, V5, and stdMCP
    Expression
    Mammalian
    Mutation
    Synonymized serine to prevent autoediting of ADAR…
    Promoter
    UBC
    Available Since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    154787-plasmid-map-sequence-id-306329

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  17. pLex307-UCOE-SFFV-puroR-MCP-MLLx3-HSF1

    Plasmid
    #218558
    Purpose
    Encodes SFFV promoter-driven MCP-MLLx3-HSF1 with an N-terminal bipartite SV40 NLS, along with puromycin resistance
    Depositor
    Alejandro Chavez
    Insert
    MCP-MLLx3-HSF1
    Use
    Lentiviral
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…
    Available Since
    Aug. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    218558-plasmid-map-sequence-id-431931

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  18. pLex307-UCOE-SFFV-puroR-MCP-P65-HSF1

    Plasmid
    #218559
    Purpose
    Encodes SFFV promoter-driven MCP-P65-HSF1 with an N-terminal bipartite SV40 NLS, along with puromycin resistance
    Depositor
    Alejandro Chavez
    Insert
    MCP-P65-HSF1
    Use
    Lentiviral
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…
    Available Since
    Aug. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    218559-plasmid-map-sequence-id-430697

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  19. phage - ubc - nls - 2xmcp - egfp - cMycBirA*

    Plasmid
    #131133
    Purpose
    BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.
    Depositor
    Ralf Jansen
    Insert
    NLS-HA-2xMCP-EGFP-cMycBirA*
    Use
    Lentiviral
    Tags
    NLS, HA, cMyc (N-terminal of BirA*)
    Expression
    Mammalian
    Mutation
    BirA* = BIrA >R118G
    Promoter
    human ubiquitin C (UBC)
    Available Since
    Aug. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    131133-plasmid-map-sequence-id-255501

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  20. pLex307-UCOE-SFFV-puroR-MCP-HSF1-VIRF2

    Plasmid
    #218557
    Purpose
    Encodes SFFV promoter-driven MCP-HSF1-VIRF2 with an N-terminal bipartite SV40 NLS, along with puromycin resistance
    Depositor
    Alejandro Chavez
    Insert
    MCP-HSF1-VIRF2
    Use
    Lentiviral
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…
    Available Since
    Aug. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    218557-plasmid-map-sequence-id-430698

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  23. lentiGuide-Puro PRMT5-CRISPRi

    Plasmid
    #164637
    Purpose
    lentiGuide-Puro backbone with cloned target sequence for human PRMT5. The insert is: 5´- CACCGAGCCGCGTGTCCAGCGGGA-3. sgRNA achieves a downregulation of PRMT5 in combination with dCAS9-KRAB-MCP2
    Depositor
    Günter Schneider
    Insert
    target guide sequence PRMT5 inhibition (PRMT5 Human)
    Use
    CRISPR and Lentiviral; Interference
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    164637-plasmid-map-sequence-id-326103

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  28. pMH205

    Plasmid
    #122861
    Purpose
    Binary expression vector containing pSUC2:MS2-VP64:tRBCS followed by GreenGate A-G sequences for insertion of additional modules
    Depositor
    Markus Schmid
    Article
    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do
    Insert
    pSUC2:MS2-VP64:tRBCS
    Use
    Golden gate compatible plant transformation vector
    Available Since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    122861-plasmid-map-sequence-id-239404

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  29. pMH186

    Plasmid
    #122837
    Purpose
    MS2-NLS (no stop codon) with GreenGate C-D flanking sequences
    Depositor
    Markus Schmid
    Article
    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do
    Insert
    MS2-NLS, codon-optimised for Arabidopsis
    Use
    Golden gate compatible cloning vector
    Tags
    Nuclear localisation signal
    Available Since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    122837-plasmid-map-sequence-id-239367

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  37. pMH183

    Plasmid
    #122856
    Purpose
    MS2-modified guide RNA targeting the FT promoter with GreenGate D-E flanking sequences
    Depositor
    Markus Schmid
    Article
    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do
    Insert
    MS2-modified sgRNA targeting the Arabidopsis FT promoter
    Use
    Golden gate compatible cloning vector
    Available Since
    June 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    122856-plasmid-map-sequence-id-247655

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  38. pMH184

    Plasmid
    #122857
    Purpose
    MS2-modified guide RNA targeting the FT promoter with GreenGate E-F flanking sequences
    Depositor
    Markus Schmid
    Article
    Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do
    Insert
    MS2-modified sgRNA targeting the Arabidopsis FT promoter
    Use
    Golden gate compatible cloning vector
    Available Since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    122857-plasmid-map-sequence-id-239400

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Showing: 681 - 720 of 1286 results