We narrowed to 25,897 results for: Nes
-
Plasmid#182965PurposeThe 3rd generation of rabies vector expressing Flpo recombinaseDepositorInsertFlpo recombinase
UseRabiesAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_010-TetO-NLS-RfxCas13d-2A-mCherry-NLS-WPRE-EFS-rtTA3-2A-Blast
Plasmid#228555PurposeExpresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleusDepositorInsertRfxCas13d
UseLentiviralTagsNLS-HA and P2A-mCherryAvailable SinceAug. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETM33_PEX14_Pex14
Plasmid#178484PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_011-TetO-NLS-inactive-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast
Plasmid#228557PurposeExpresses inactive RfxCas13d in mammalian cells for binding of target RNAs in combination with compatible crRNAs. Localized to the nucleusDepositorInsertdRfxCas13d
UseLentiviralTagsNLS-HAMutationR239A/H244A/R858A/H863AAvailable SinceAug. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETM33_SIN3A_PAH
Plasmid#178486PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLAG-Claspin
Plasmid#12659DepositorAvailable SinceMay 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDN34
Plasmid#61343PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/PKIt NES)DepositorInsertmCherry
TagsGS linker-AsLov2-biNLS2 and PKIt NESExpressionMammalianPromoterCMVAvailable SinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pB-TREtight-EGFP
Plasmid#182967PurposeFor making cell lines for titering pRV∆L-5tTA virusDepositorInsertEGFP
UseUnspecifiedAvailable SinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-U-TVA950
Plasmid#115236PurposeLentiviral transfer plasmid for expression of TVA950DepositorInsertTVA950
ExpressionMammalianAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_009-TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-EGFP
Plasmid#223174PurposeExpresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleusDepositorInsertRfxCas13d
UseLentiviralTagsNLS-HAAvailable SinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-pGK-Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro
Plasmid#115684PurposeNanoLuc-Firefly Luciferase fusion with constitutive nuclear expression for calibration of Nanoluc signal to Firefly Luciferase signal. H2B-mCherry marks chromatin with red fluorescence.DepositorInsertNluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro
UseLentiviralAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cyto-B
Plasmid#209858PurposeDimerization dependent fluorescent protein B fused to NESDepositorInsertddGFP B
TagsNES sequence LALKLAGLDIGSExpressionMammalianPromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM20C
Plasmid#135487PurposeAgrobacterium T-DNA with divergent Spun H2A/Bpr driving H2B-tdTomato and hphDepositorInserthph SpunH2A/Bpr H2B-tdTomato
TagstdTomatoExpressionBacterial and YeastPromoterSpunH2A/Bpr (divergent)Available SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSimpleII-NLS-NmCas9-HA-NLS(s)
Plasmid#47867PurposeThis plasmid encode NmCas9 with two NLS and a HA tagDepositorInsertNmCas9
UseCRISPRTagsHA and NLSExpressionMammalianPromoterEF1aAvailable SinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_012-TetO-NLS-inactive-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast
Plasmid#228558PurposeExpresses inactive hfCas13d in mammalian cells for binding of target RNAs in combination with compatible crRNAs. Localized to the nucleusDepositorInsertdhfCas13d (N2V8)
UseLentiviralTagsNLS-HAMutationR239A/H244A/R858A/H863AAvailable SinceAug. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETM33_YES1_SH3
Plasmid#178492PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM33_SUFU_Sufu
Plasmid#178488PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB-CAG-TEVp-IRES-mCherry
Plasmid#174377PurposeFor making TEVP cell line with hypBaseDepositorInsertTEV Protease
ExpressionMammalianAvailable SinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB22
Plasmid#61342PurposeMammalian expression of mCherry fused to LINuS, a light-inducible nuclear localization signal (cMycP1A NLS/PKIt NES)DepositorInsertmCherry
TagsGS linker-AsLov2-cMyc1A NLS and PKIt NESExpressionMammalianPromoterCMVAvailable SinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM22C
Plasmid#135488PurposeAgrobacterium T-DNA with divergent H2A/Bpr driving LifeAct-tdTomato and hphDepositorInserthph SpunH2A/Bpr LifeAct-tdTomato
TagsLifeActExpressionBacterial and YeastPromoterSpunH2A/Bpr (divergent)Available SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_OXSR1_CCT
Plasmid#178482PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNB785
Plasmid#60164PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter.DepositorInsertFLuc-yEVenus-PEST
TagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterSIC1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB787
Plasmid#60152PurposeFirefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.DepositorInsertFLuc-yEVenus-PEST
TagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterLEU1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB789
Plasmid#60166PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.DepositorInsertFLuc-yEVenus-PEST
TagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterRNR1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
Human Mevalonate diphosphate decarboxylase (MDD)
Plasmid#52431Purposebacterial expression of human Mevalonate diphosphate decarboxylase (MDD)DepositorAvailable SinceApril 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTE_10 - pCMV-Cas9_only
Plasmid#252006PurposeMammalian expression of Cas9DepositorInsertCas9
UseCRISPRExpressionMammalianMutationNAAvailable SinceMarch 4, 2026AvailabilityAcademic Institutions and Nonprofits only -
pWN103
Plasmid#233860PurposeMoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NES-NS3-V1-NLS-VP48-2XOaf1-tADH1DepositorInsertpTDH3-LexA-NES-NS3-V1-NLS-VP48-2XOaf1-tADH1
ExpressionYeastAvailable SinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWN104
Plasmid#233861PurposeMoClo-YTK; ConLS/R1 cassette; NS3 systems; pTDH3-LexA-NES-NS3-V2-NLS-VP48-2XOaf1-tADH1DepositorInsertpTDH3-LexA-NES-NS3-V2-NLS-VP48-2XOaf1-tADH1
ExpressionYeastAvailable SinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETM33_SPSB1_SPRY
Plasmid#178487PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TREtight-H2b-emiRFP670-P2A-TEVP
Plasmid#174379PurposeAAV virus for expressing TEVP and H2b-emiRFP670DepositorInsertTEVP
UseAAVAvailable SinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRV∆G-NPEST-4Cre
Plasmid#174380PurposeRabies vector for expressing Cre recombinase and N gene with PEST domainDepositorInsertCre recombinase
UseRabiesAvailable SinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cyto-ExRai-CKAR2
Plasmid#236097PurposeCytosol-targeted enhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells.DepositorInsertExRai-CKAR2-NES
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceMay 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast
Plasmid#138149PurposeExpresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleusDepositorInsertCasRx
UseLentiviralTagsNLS-HAAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE
Plasmid#138150PurposeFor cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaaDepositorInserthU6_Rfxcas13d-DR1_BsmBI
UseLentiviralMutationDR1 A>TAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2
Plasmid#75112Purposelenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6 and EF1AAvailable SinceMay 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cyto-ExRai-AktAR2
Plasmid#184048PurposeCytosol-targeted ExRai-AktAR2.DepositorInsertCyto-ExRai-AktAR2
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTE_01 - pLenti_EFS-Cas9-XTEN-ZRANB3-2A-BlastR
Plasmid#251997PurposeLentiviral mammalian expression of Cas9-ZRANB3 TruEditorDepositorInsertCas9-ZRANB3
UseCRISPR and LentiviralMutationNAAvailable SinceMarch 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_008-TetO-NLS-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast
Plasmid#223173PurposeExpresses hfCas13d (N2V8) in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus.DepositorInserthfCas13d (N2V8)
UseLentiviralTagsNLS-HAAvailable SinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE
Plasmid#138151PurposeFor cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaaDepositorInserthU6_Rfxcas13d-DR_BsmBI
UseLentiviralAvailable SinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNAGuide_004-hU6-RfxCas13d-DR1-BsmBI-EFS-EGFP-P2A-Puro-WPRE
Plasmid#223175PurposeFor cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaaDepositorInserthU6_Rfxcas13d-DR1_BsmBI
UseLentiviralMutationDR1 A>TAvailable SinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
p2xFLAGhYAP1
Plasmid#17791DepositorAvailable SinceMay 9, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLV-CAG-FLEX-BFP2-(mCherry)’
Plasmid#115234PurposeLentiviral transfer plasmid for expression of mTagBFP2 before Cre recombination and mCherry after itDepositorInsertmTagBFP2 and mCherry
ExpressionMammalianAvailable SinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pN1-GCaMP6m-XC
Plasmid#111543PurposeImproved genetic encoded calcium sensor based on GCaMP6m. Also called GCaMP-XDepositorInsertGCaMP6m-XC
ExpressionMammalianAvailable SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Atg7
Plasmid#24921DepositorAvailable SinceJune 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
VPR_dCas9
Plasmid#134601PurposeExpress CHO codon-optimized dCas9 fused to VPR for CRISPR activation. 2A linked ZsGreen1 markerDepositorInsertVPR-dCas9-2A-ZsGreen-DR
TagsVPR and ZsGreen1-DRExpressionMammalianPromoterCMVAvailable SinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Atg5
Plasmid#24922DepositorAvailable SinceJune 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLentiRNACRISPR_003 - hU6-DR_BsmBI-EFS-PspCas13b-NLS-2A-Puro-WPRE
Plasmid#138145PurposeExpresses PspCas13b in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BsmBI.DepositorInsertPspCas13b
UseLentiviralTagsNLSAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-TnY
Plasmid#163975PurposeIPTG-inducible expression of Transposon TnY fused to Mxe Intein and Chitin-binding domain.DepositorInsertTnY
TagsMxe intein - Chitin-binding domainExpressionBacterialAvailable SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10625
Plasmid#239268PurposeExpresses NES-dCas13b-ABI for CRISPR-TO perturbation in primary mouse cortical neuronsDepositorInsertNES-dPspCas13b-EGFP-ABI
TagsFlag tag and V5 tagExpressionMammalianMutationH133A and H1058A in PspCas13bPromoterCAGAvailable SinceSept. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLentiCCAT1-Red
Plasmid#226521PurposeCCAT1 transgene with fluorescent protein mKate2DepositorInsertEF1a_mKate2_CMV_CCAT1 (CCAT1 Human)
UseLentiviralTagsmKate2ExpressionMammalianPromoterCMVAvailable SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only