We narrowed to 11,210 results for: NSI

1. pCMV-MfPylRS (human-opti)

   Plasmid

   #164081

   Purpose

   To express a Methanosarcina flavescens derived PylRS in mammalian cells for genetic code expansion

   Depositor

   Tao Liu

   Article

   Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

   Insert

   MfPylRS(human-opti)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS-Nvec-codonopti-Cas9-2xNLS

   Plasmid

   #141108

   Purpose

   Plasmid containing insert for the IVT of nuclear localized Cas9 mRNA with codon optimization for Nematostella vectensis

   Depositor

   Matthew Gibson

   Article

   Hill et al Methods Mol Biol. 2022;2450:437-465. doi

   Insert

   Nematostella codon optimized Cas9
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   All amino acids coded for by codons optimized for…

   Promoter

   T7

   Available since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. MDH1-miR-181a1-Dusp6

   Plasmid

   #17849

   Depositor

   Chang-Zheng Chen

   Article

   Li et al Cell. 2007 Apr 6. 129(1):147-61.

   Insert

   Dusp6-EGFP (Dusp6 Mouse)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEMHE:hTRAAK(Q258K)

   Plasmid

   #133077

   Purpose

   X. laevis expression vector. It will generate the human TRAAK channel

   Depositor

   Dan Minor

   Article

   Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

   Insert

   Potassium channel subfamily K member 2 (KCNK2, TREK-1), Q258K (Kcnk2 Mouse)
   

   Use

   Tags

   Expression

   Mutation

   Q258K

   Promoter

   T7

   Available since

   Oct. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-MtPylRS (human-opti)

   Plasmid

   #164080

   Purpose

   To express a Methanosarcina thermophila derived PylRS in mammalian cells for genetic code expansion

   Depositor

   Tao Liu

   Article

   Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

   Insert

   MtPylRS-478(human-opti)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-MfBulKRS (human-opti)

   Plasmid

   #164196

   Purpose

   To express a Methanosarcina flavescens derived BulKRS in mammalian cells for genetic code expansion

   Depositor

   Tao Liu

   Article

   Hu et al ACS Synth Biol. 2020 Oct 16;9(10):2723-2

   Insert

   Mf BulKRS(human-opti)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Y269A;Y347F

   Promoter

   CMV

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. MDH1-shDusp5-2-H2Kb2

   Plasmid

   #17853

   Depositor

   Chang-Zheng Chen

   Article

   Li et al Cell. 2007 Apr 6. 129(1):147-61.

   Insert

   shDusp5 (DUSP5 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 16, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPICZ:hTRAAK(G124I)

   Plasmid

   #130672

   Purpose

   P. pastoris expression vector. It will generate the human TRAAK channel (1-300) fused to a C-terminal GFP

   Depositor

   Dan Minor

   Article

   Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

   Insert

   KCNK4 (KCNK4 Human)
   

   Use

   Tags

   SNS- 3C_cleavage_site-TAAA-GFP

   Expression

   Yeast

   Mutation

   N104Q, N108Q, G124I

   Promoter

   Available since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSTAP-seq_fly-zfh1_spikeIn-1

   Plasmid

   #86387

   Purpose

   Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter

   Depositor

   Alexander Stark

   Article

   Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

   Insert

   D. melanogaster zfh1 enhancer & D. pseudoobscura eve core promoter
   

   Use

   Stap-seq spike in vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSTAP-seq_fly-zfh1_spikeIn-3

    Plasmid

    #86389

    Purpose

    Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster zfh1 enhancer & D. pseudoobscura ush core promoter -TSS#1
    

    Use

    Stap-seq spike in vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSTAP-seq_fly-zfh1_spikeIn-4

    Plasmid

    #86390

    Purpose

    Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster zfh1 enhancer & D. pseudoobscura ush core promoter -TSS#2
    

    Use

    Stap-seq spike in vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSTAP-seq_fly-zfh1_spikeIn-2

    Plasmid

    #86388

    Purpose

    Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster zfh1 enhancer & D. pseudoobscura CG32369 core promoter
    

    Use

    Stap-seq spike in vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. CC1

    Plasmid

    #63713

    Purpose

    Wild-type voltage sensing domain from Ciona voltage sensing phosphatase fused to super ecliptic A227D with restriction site flanking each transmembrane domain

    Depositor

    Bradley Baker

    Article

    Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi

    Insert

    CC1
    

    Use

    Tags

    Super ecliptic pHlorin A227D

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pET-Cb2

    Plasmid

    #53529

    Purpose

    Expresses an insect mitochondrial arginyl-tRNA synthetase in E.coli

    Depositor

    Gabor Igloi

    Article

    Leisinger et al Insect Biochem Mol Biol. 2013 Dec;43(12)

    Insert

    mitochondrial arginyl-tRNA synthetase
    

    Use

    Tags

    His Tag and Thioredoxin

    Expression

    Bacterial

    Mutation

    changed Ser4 to Ala4 to permit NcoI cloning

    Promoter

    T7

    Available since

    July 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPICZ:hTRAAK(W262S)

    Plasmid

    #130670

    Purpose

    P. pastoris expression vector. It will generate the human TRAAK channel (1-300) fused to a C-terminal GFP

    Depositor

    Dan Minor

    Article

    Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

    Insert

    KCNK4 (KCNK4 Human)
    

    Use

    Tags

    SNS- 3C_cleavage_site-TAAA-GFP

    Expression

    Yeast

    Mutation

    N104Q, N108Q, W262S

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIRES:hTRAAK(W262S)

    Plasmid

    #133078

    Purpose

    Mammalian expression vector. It will generate the human TRAAK channel full lenght W262S mutant

    Depositor

    Dan Minor

    Article

    Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

    Insert

    Potassium channel subfamily K member 4 (KCNK4, TRAAK) W262S (KCNK4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIRES:hTRAAK(G124I)

    Plasmid

    #133079

    Purpose

    Mammalian expression vector. It will generate the human TRAAK channel full lenght G124I mutant

    Depositor

    Dan Minor

    Article

    Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

    Insert

    Potassium channel subfamily K member 4 (KCNK4, TRAAK) G124I (KCNK4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G124I

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus

    Plasmid

    #61629

    Purpose

    R2D2: Expresses a fusion of auxin-sensitive DII to nuclear 3xVenus and auxin-insensitive mDII to nuclear tdTomato, both from the Arabidopsis RPS5A promoter

    Depositor

    Dolf Weijers

    Article

    Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:

    Inserts

    mDII
    ntdTomato
    DII
    n3xVenus
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    RPS5A

    Available since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-PGK-Venus-Akaluc (neo)

    Plasmid

    #124701

    Purpose

    Aka-Luciferase reporter

    Depositor

    Roland Friedel

    Article

    Bozec et al Neurooncol Adv. 2020 Oct 10;2(1):vdaa134

    Insert

    Venus-Akaluciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hPGK

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Flag-Bcl2

    Plasmid

    #18003

    Depositor

    Clark Distelhorst

    Article

    Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

    Insert

    Bcl-2 (BCL2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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