We narrowed to 6,865 results for: addgene/1000

1. Flag-M4-AR-S650D

   Plasmid

   #171246

   Purpose

   Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser650Asp (AR-S650D)

   Depositor

   Steven Balk

   Article

   Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

   Insert

   3xFlag-tagged human androgen receptor Ser650Asp mutant (AR Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   AR-Ser650Asp (AR-S650D)

   Promoter

   CMV

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Flag-M4-AR-S650A

   Plasmid

   #171245

   Purpose

   Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant: AR-Ser650Ala (AR-S650A)

   Depositor

   Steven Balk

   Article

   Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

   Insert

   3xFlag-tagged human androgen receptor Ser650Ala mutant (AR Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   AR-Ser650Ala (AR-S650A)

   Promoter

   CMV

   Available since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. DAG1-bio-His

   Plasmid

   #51651

   Purpose

   Expresses full-length Dystroglycan precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   DAG1 (DAG1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. plentiCRISPR-v2 Puro EIF2AK1/HRI_sgRNA

   Plasmid

   #218529

   Purpose

   sgRNA targeting human EIF2AK1/HRI

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   EIF2AK1 (EIF2AK1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3HA-AR-LBD

   Plasmid

   #89111

   Purpose

   mammalian expression of human androgen receptor ligand binding domain amino acid residues 624-919 with N-terminal double HA tag

   Depositor

   Elizabeth Wilson

   Article

   He et al J Biol Chem. 1999 Dec 24;274(52):37219-2

   Insert

   hAR ligand binding domain (AR Human)
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   contains Ligand Binding Domain (624-919)

   Promoter

   CMV

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBiex-1 AC30_eGFP

   Plasmid

   #82715

   Purpose

   Expresses a GFP-tagged recombinant adenylyl cyclase.

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

   Insert

   Adenylyl Cyclase V/II (ADCY2, ADCY5 Human)
   

   Use

   Tags

   FLAG purification tag and eGFP

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   T7 Promoter

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-hAR-CA576-Z

   Plasmid

   #89108

   Purpose

   mammalian expression of full-length human androgen receptor with DNA binding domain mutation CA576

   Depositor

   Elizabeth Wilson

   Article

   Zhou et al J Biol Chem. 1994 May 6;269(18):13115-23

   Insert

   hAR-C576A (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C576A

   Promoter

   CMV

   Available since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

8. pCMV-AR-R405A

   Plasmid

   #126052

   Purpose

   Expresses human androgen receptor (R405A) in mammalian cells

   Depositor

   Elizabeth Wilson

   Article

   Lagarde et al J Biol Chem. 2012 Mar 30;287(14):10905-1

   Insert

   AR (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R405A

   Promoter

   CMV

   Available since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB CAG Cdk6 R31C-IRES-EGFP

   Plasmid

   #153946

   Purpose

   piggyBac transposon vector with CAG promoter expressing Cdk6 (R31C mutant) and EGFP

   Depositor

   Luca Tiberi

   Article

   Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

   Insert

   Cdk6 (Cdk6 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R31C

   Promoter

   CAG

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Flag-M4-AR-W741C-S81A

    Plasmid

    #171248

    Purpose

    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Ala (AR-W741C-S81A)

    Depositor

    Steven Balk

    Article

    Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

    Insert

    3xFlag-tagged human androgen receptor Trp741Cys-Ser81Ala mutant (AR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-Trp741Cys-Ser81Ala (AR-W741C-S81A)

    Promoter

    CMV

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Flag-M4-AR-W741C-S81D

    Plasmid

    #171249

    Purpose

    Mammalian expression of 3xFlag-tagged human androgen receptor phosphorylation mutant that binds to bicalutamide: AR-Trp741Cys-Ser81Asp (AR-W741C-S81D)

    Depositor

    Steven Balk

    Article

    Chen et al J Biol Chem. 2012 Mar 9;287(11):8571-83.

    Insert

    3xFlag-tagged human androgen receptor Trp741Cys-Ser81Asp mutant (AR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-Trp741Cys-Ser81Asp (AR-W741C-S81D)

    Promoter

    CMV

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-AR-R405S

    Plasmid

    #126051

    Purpose

    Expresses human androgen receptor (R405S) in mammalian cells

    Depositor

    Elizabeth Wilson

    Article

    Lagarde et al J Biol Chem. 2012 Mar 30;287(14):10905-1

    Insert

    AR (AR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R405S

    Promoter

    CMV

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKLV2-U6gRNA5(hPKR_2g)-PGKpuro2AmCherry-W

    Plasmid

    #208435

    Purpose

    Lentiviral gRNA plasmid targeting human PKR gene, co-expression of mCherry tag

    Depositor

    Sok Ching Cheong

    Article

    Yee et al Mol Carcinog. 2025 Jun;64(6):1066-1077.

    Insert

    PKR (EIF2AK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    human U9

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDestTol2-actinb-kcnj13-M135R-EGFP

    Plasmid

    #164944

    Purpose

    Tol2 construct: actinb (actb2) promoter driven zebrafish gain-of-function kcnj13 mutant, M135R

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    kcnj13 (kcnj13 Zebrafish)
    

    Use

    Tol2 transposon destination vector

    Tags

    EGFP

    Expression

    Mutation

    Stop codon deleted; M135R

    Promoter

    actinb (actb2) promoter

    Available since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDestTol2-actinb-Hs.KCNJ13-IRES-EGFP

    Plasmid

    #164945

    Purpose

    Tol2 construct: actinb (actb2) promoter driven human KCNJ13 and EGFP”. This will reflect the linkage of KCNJ13 and EGFP

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    KCNJ13 (KCNJ13 Human)
    

    Use

    Tol2 transposon destination vector

    Tags

    IRES-EGFP

    Expression

    Mutation

    Stop codon deleted

    Promoter

    actinb (actb2) promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDestTol2-actinb-kcnj13-Q153H-EGFP

    Plasmid

    #164943

    Purpose

    Tol2 construct: actinb (actb2) promoter driven zebrafish loss-of-functionkcnj13 mutant, Q153H and M154I

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    kcnj13 (kcnj13 Zebrafish)
    

    Use

    Tol2 transposon destination vector

    Tags

    EGFP

    Expression

    Mutation

    Stop codon deleted; Q153H; M154I

    Promoter

    actinb (actb2) promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDestTol2-actinb-kcnj13-T131A-EGFP

    Plasmid

    #164942

    Purpose

    Tol2 construct: actinb (actb2) promoter driven zebrafish loss-of-function kcnj13 mutant, T131A and G133R

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    kcnj13 (kcnj13 Zebrafish)
    

    Use

    Tol2 transposon destination vector

    Tags

    EGFP

    Expression

    Mutation

    Stop codon deleted; T131A; G133R

    Promoter

    actinb (actb2) promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LV-AR

    Plasmid

    #213819

    Purpose

    Lenti-viral expression of human androgen receptor wild-type (WT).

    Depositor

    Steven Balk

    Article

    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

    Insert

    AR (human androgen receptor) (AR Human)
    

    Use

    Lentiviral

    Tags

    Flag (N terminal on backbone)

    Expression

    Mammalian

    Mutation

    No, it is AR wild-type.

    Promoter

    EF-1a

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HA-HA-AR-DBD

    Plasmid

    #171238

    Purpose

    Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain (DBD)

    Depositor

    Steven Balk

    Article

    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

    Insert

    2xHA-tagged human androgen receptor trancate:DNA-binding domain (AR Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    AR truncate

    Promoter

    CMV

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HA-HA-AR-NTD

    Plasmid

    #171235

    Purpose

    Mammalian expression of 2xHA-tagged human androgen receptor trancate: AR N-terminal-domain (NTD)

    Depositor

    Steven Balk

    Article

    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

    Insert

    2xHA-tagged human androgen receptor trancate: N-terminal-domain (AR Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    AR truncate

    Promoter

    CMV

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge