We narrowed to 5,566 results for: donor

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AAV_Sox2 HMEJ donor

Plasmid

#97322

Purpose

HMEJ donor for fusing a p2A-mCherry reporter to mouse Sox2. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Sox2 HMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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AAV_Actb NHEJ donor_U6_sgRNA_EF1a_GFP_polyA

Plasmid

#97310

Purpose

NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb. EGFP driven by EF1a promoter and U6-driven sgRNAs targeting Actb. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Actb NHEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Promoter

U6, EF1a

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pSL0721 (pDonor_L1-105)

Plasmid

#130645

Purpose

Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene and shortened left end. Total transposon size = 933 bp.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchCAST donor DNA (short L)

Use

CRISPR; Transposon

Expression

Bacterial

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV_Donor/Reporter-COL4A5

Plasmid

#130280

Purpose

COL4A5 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A5 donor template

Depositor

Silvestro Conticello

Article

Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

Insert

mCherry-eGFP

Use

AAV

Promoter

CMV

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pTLR-donor-UAS

Plasmid

#119001

Purpose

Repair template for the TLR reporter with 10 UAS binding sites for yeast Gal4

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

mVenus

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pTLR-donor-tetO

Plasmid

#119000

Purpose

Repair template for the TLR reporter with 7 tet operator elements

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

mVenus

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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AAV_Dbh HMEJ donor

Plasmid

#97320

Purpose

HMEJ donor for fusing a p2A-mCherry-WPRE reporter to mouse Dbh. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Dbh HMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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mGDF8 donor mCherry-PA

Plasmid

#72596

Purpose

Plasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin cassette.

Depositor

Renzhi Han

Article

Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

Insert

mCherry-puromycin cassetter

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only
WT FLAG-HaloTag-RTEL1_DonorVector

Plasmid

#247170

Purpose

HR Donor for WT C-terminal FLAG-HaloTag-RTEL1 fusion in human cells. Includes Puro selection marker.

Depositor

Thomas Cech

Article

Wu et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

WT FLAG-HaloTag-RTEL1 (RTEL1 Human)

Promoter

Endogenous RTEL1 promoter

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pLP857_α-syn:oScarlet KI donor

Plasmid

#239403

Purpose

AAV vector for SpCas9-mediated CRISPR KI of oScarlet at the C-terminus of mouse α-syn

Depositor

Subhojit Roy

Article

Parra-Rivas et al STAR Protoc. 2025 Jul 21;6(3):103945. do

Insert

oScarlet

Use

AAV and CRISPR

Tags

oScarlet

Expression

Mammalian

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA-CAG Donor

Plasmid

#241374

Purpose

Bxb1-GA donor plasmid with CAG promoter, WPRE and polyA signal for constitutive transgene expression (empty)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT-CAG Donor

Plasmid

#241373

Purpose

Bxb1-GT donor plasmid with CAG promoter, WPRE and polyA signal for constitutive transgene expression (empty)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-giantin-ATG_donor

Plasmid

#222316

Purpose

Perform knock-in by homologous recombination in giantin/GOLGB1 gene.

Depositor

Franck Perez

Article

Fourriere et al J Cell Sci. 2016 Sep 1;129(17):3238-50.

Insert

EGFP and homology arms for giantin/GOLGB1

Use

CRISPR

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pDonor-FMR1-dCGG0

Plasmid

#222965

Purpose

To create 0CGG FMR1 cell line

Depositor

Xinyu Zhao

Article

Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

Insert

FMR1 homologous template no CGG repeats (FMR1 Human)

Use

CRISPR

Mutation

CGG repeats deleted from 5' UTR

Available Since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pJT310 AAV-BFPdonor

Plasmid

#202057

Purpose

AAV HDR donor

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

AAV-BFPdonor

Use

AAV; Aav packaging plasmid

Expression

Mammalian

Mutation

ITR deletion

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDonor-PRF1-Exon3

Plasmid

#209075

Purpose

Targeting vector for the human PRF1 locus to replace exon 3 with repaired exon 3

Depositor

Klaus Rajewsky

Article

Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

Insert

PRF1-Exon3 HDRT (PRF1 Human)

Use

CRISPR

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pJMC-L2-Donor-Esp3I

Plasmid

#197841

Purpose

Donor vector to restore L2 destination via Esp3I

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

None

Use

Unspecified

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-L2-Donor-BsaI

Plasmid

#197840

Purpose

Donor vector to restore L2 destination via BsaI

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

None

Use

Unspecified

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pJET-osr1-KI-donor

Plasmid

#184875

Purpose

Donor template for knock-in of p2a-CreERT2 into the medaka osr1 locus via CRISPR/Cas9-mediated homology directed repair

Depositor

Christoph Winkler

Article

Hui et al Development. 2022 May 20. pii: 275483. d

Inserts

Odd-Skipped Related Transcription Factor 1 5' homology arm
Odd-Skipped Related Transcription Factor 1 3' homology arm
p2a-CreERT2
Myosin light chain 2 promoter
EGFP

Use

CRISPR and Cre/Lox

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pTOPO-col10a1-KI-donor

Plasmid

#184874

Purpose

Donor template for knock-in of p2a-CreERT2 into the medaka col10a1 locus via CRISPR/Cas9-mediated homology directed repair

Depositor

Christoph Winkler

Article

Hui et al Development. 2022 May 20. pii: 275483. d

Inserts

Collagen10a1 5' homology arm
Collagen10a1 3' homology arm
p2a-CreERT2
Myosin light chain 2 promoter
eGFP

Use

CRISPR and Cre/Lox

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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