Addgene

Showing: 341 - 360 of 9015 results

Results per page:

Sort results by:

PH-PLCD1-GFP

Plasmid

#51407

Purpose

Is a biosensor for PI(4,5)P2

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

PH-PLCD1 (aa 1–170) (PLCD1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
Plasma membrane-targeted optoPLD

Plasmid

#140114

Purpose

Encodes CRY2-mCherry-PLD-P2A-CIBN-CAAX for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Use

Tags

CAAX, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
PH-Akt(R25C)-GFP

Plasmid

#51466

Purpose

Non-PIP3 binding control for PH-Btk-GFP

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

AKT (aa 1–164)

Use

Tags

EGFP

Expression

Mammalian

Mutation

R25C

Promoter

CMV

Available since

March 26, 2014

Availability

Academic Institutions and Nonprofits only
Endoplasmic reticulum-targeted optoPLD

Plasmid

#140060

Purpose

Encodes CRY2-mCherry-PLD-P2A-CIBN-Sac1(TM) for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Use

Tags

CIBN, CRY2, P2A, Sac1(TM), and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Endosome-targeted optoPLD

Plasmid

#140056

Purpose

Encodes CRY2-mCherry-PLD-P2A-CIBN-2xFYVE for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Use

Tags

2xFYVE, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Golgi-targeted optoPLD

Plasmid

#140058

Purpose

Encodes CRY2-mCherry-PLD-P2A-CIBN-Sialyltransferase(TM) for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Use

Tags

CIBN, CRY2, P2A, Sialyltransferase(TM), and mCher…

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Golgi-targeted optodPLD

Plasmid

#140057

Purpose

Encodes CRY2-mCherry-dPLD-P2A-CIBN-Sialyltransferase(TM) for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Use

Tags

CIBN, CRY2, P2A, Sialyltransferase(TM), and mCher…

Expression

Mammalian

Mutation

H170A (catalytic dead)

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Endosome-targeted optodPLD

Plasmid

#140055

Purpose

Encodes CRY2-mCherry-dPLD-P2A-CIBN-2xFYVE for mammalian expression

Depositor

Jeremy Baskin

Article

Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Phospholipase D

Use

Tags

2xFYVE, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Mutation

H170A (catalytic dead)

Promoter

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p15aC-4D1D

Plasmid

#107864

Purpose

Bacterial expression of PIS and PI4K

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Inserts

PIS
phosphatidylinositol 4-kinase β

Use

Tags

myc

Expression

Bacterial

Mutation

Promoter

proD

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
p40 R58Q/W207R

Plasmid

#19014

Depositor

Michael Yaffe

Article

Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19

Insert

p40phox (NCF4 Human)

Use

Tags

His

Expression

Bacterial

Mutation

R58Q/W207R

Promoter

Available since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
p40 R58Q

Plasmid

#19015

Depositor

Michael Yaffe

Article

Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19

Insert

p40phox (NCF4 Human)

Use

Tags

His

Expression

Bacterial

Mutation

R58Q

Promoter

Available since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
p40 W207R

Plasmid

#19016

Depositor

Michael Yaffe

Article

Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19

Insert

p40phox (NCF4 Human)

Use

Tags

His

Expression

Bacterial

Mutation

W207R

Promoter

Available since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
p40 D293K

Plasmid

#19017

Depositor

Michael Yaffe

Article

Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19

Insert

p40phox (NCF4 Human)

Use

Tags

His

Expression

Bacterial

Mutation

D293K

Promoter

Available since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
p40 R58Q/E259A

Plasmid

#19020

Depositor

Michael Yaffe

Article

Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19

Insert

p40phox (NCF4 Human)

Use

Tags

His

Expression

Bacterial

Mutation

R58Q/E259A

Promoter

Available since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
p40 D293K/E259A

Plasmid

#19021

Depositor

Michael Yaffe

Article

Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19

Insert

p40phox (NCF4 Human)

Use

Tags

His

Expression

Bacterial

Mutation

D293K/E259A

Promoter

Available since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
p40 W207R/E259A

Plasmid

#19022

Depositor

Michael Yaffe

Article

Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19

Insert

p40phox (NCF4 Human)

Use

Tags

His

Expression

Bacterial

Mutation

W207R/E259A

Promoter

Available since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
p40 E259A

Plasmid

#19023

Depositor

Michael Yaffe

Article

Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19

Insert

p40phox (NCF4 Human)

Use

Tags

His

Expression

Bacterial

Mutation

E259A

Promoter

Available since

Aug. 25, 2008

Availability

Academic Institutions and Nonprofits only
Human PNP his-tagged

Plasmid

#64076

Purpose

Expression of human Purine Nucleoside Phosphorylase

Depositor

Vern Schramm

Article

Lewandowicz et al J Biol Chem. 2003 Aug 22;278(34):31465-8

Insert

Purine Nucleoside Phosphorylase (PNP Human)

Use

Tags

Histidine tag

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only
PKG I alpha WT

Plasmid

#16390

Depositor

Bernard Weinstein

Article

Deguchi et al Mol Cancer Ther. 2002 Aug . 1(10):803-9.

Insert

PKG1 alpha (PRKG1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pCiVSP(C363S)-SD64TF

Plasmid

#80334

Purpose

Expresses Ciona VSP (without phosphatase activity) in Xenopus Oocyte

Depositor

Yasushi Okamura

Article

Murata et al Nature. 2005 Jun 30;435(7046):1239-43. E

Insert

Ciona intestinalis voltage-sensing phosphatase (C363S) (vsp Ciona intestinalis)

Use

Xenopus oocyte expression

Tags

Expression

Mutation

Changed Cysteine 363 to Serine

Promoter

SP6

Available since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge