165,025 results

1. CLYBL-TO-hNGN2-BSD-mApple

   Plasmid

   #124229

   Purpose

   Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2 for differentiation of iPSCs into cortical neurons. Contains a floxed BSD-NLS-mApple selection cassette.

   Depositor

   Michael Ward

   Article

   Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

   Insert

   NGN2 (NEUROG2 Human)
   

   Expression

   Mammalian

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-FRT-TO-NOD2-EGFP

   Plasmid

   #131207

   Purpose

   mammalian expression, tet R

   Depositor

   Thomas Kufer

   Article

   Pei et al EMBO J. 2021 Jul 1;40(13):e106272. doi:

   Insert

   NOD2 (NOD2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   pCMV

   Available Since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. LRG2.1

   Plasmid

   #108098

   Purpose

   Lentiviral expression plasmid of sgRNA with GFP

   Depositor

   Christopher Vakoc

   Article

   Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 promoter for sgRNA expression and EFS promoter…

   Available Since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. p37-2iDMD-LR

   Plasmid

   #88892

   Purpose

   37°C full-length DMD luc-red plasmid. Co-expresses human dystrophin, firefly luciferase and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites and can be grown at 37°C.

   Depositor

   Michele Calos

   Article

   Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/

   Inserts

   Dystrophin (DMD Human)
   luciferase
   mCherry
   

   Expression

   Mammalian

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti6.2_mCherry_mito Grx1-roGFP2

   Plasmid

   #155044

   Purpose

   Fluorescent redox reporter

   Depositor

   Vanessa LaPointe

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Use

   Lentiviral

   Tags

   mitochondrial targeting sequence and roGFP2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcmv3-IRG1-HA

   Plasmid

   #198181

   Purpose

   Mammalian expression of HA-tagged human ACOD1 (IRG1)

   Depositor

   Yun Zhao

   Article

   Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

   Insert

   ACOD1 (ACOD1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   HA tag is added to C-terminal of target protein

   Promoter

   CMV

   Available Since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSF1389

   Plasmid

   #104962

   Purpose

   Bacterial expression plasmid of bdSENP1 protease

   Depositor

   Dirk Görlich

   Article

   Frey et al J Chromatogr A. 2014 Apr 11;1337:95-105.

   Insert

   SENP1
   

   Tags

   His14 tag and Tev protease cleavage site

   Expression

   Bacterial

   Promoter

   lacUV5

   Available Since

   Jan. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. LAMP1-mRFP-FLAG

   Plasmid

   #34611

   Purpose

   3rd gen lentiviral vector expressing a fusion protein of Lamp1, mRFP, and a FLAG tag

   Depositor

   David Sabatini

   Article

   Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

   Insert

   LAMP1 (Lamp1 Rat)
   

   Use

   Lentiviral

   Tags

   2xFlag and mRFP

   Expression

   Mammalian

   Available Since

   March 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. 3xnls-mNeonGreen

   Plasmid

   #98875

   Purpose

   In vivo visualization of the nucleus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   3xNLS
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti CMV GFP DEST (736-1)

    Plasmid

    #19732

    Purpose

    3rd gen lentiviral Gateway destination vector, expression/RNAi, eGFP co-expression

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral and RNAi; Gateway destination vector

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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