12,914 results

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pCR8/GW_TOPO_Cdk12_Untagged

Plasmid

#127176

Purpose

Untagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)

Use

Entry vector with transgene for gateway cloning

Tags

None

Promoter

None

Available Since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA-mMcl1

Plasmid

#25714

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

mMCL1 (Mcl1 Mouse)

Tags

HA

Expression

Mammalian

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only

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pP2A_NLS_sfGFP

Plasmid

#63709

Purpose

Expressing P2A_NLS_sfGFP with homologous arm of Nanog.

Depositor

Stanley Qi

Article

Yu et al Cell Stem Cell. 2015 Feb 5;16(2):142-7.

Insert

Nanog homologous arm, p2A, NLS, sfGFP, Nanog homologous arm

Use

Just a donor dna with nanog homologous arm for kn…

Tags

sfGFP

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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pLH116

Plasmid

#22651

Depositor

Lynn Hudson

Article

Hudson et al Proc Natl Acad Sci U S A. 1987 Mar . 84(

Insert

Proteolipid Protein (Plp1 Mouse)

Use

Cloning vector

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miRFP713-P2A-GFP

Plasmid

#197163

Purpose

Expresses the protein of miRFP713-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

miRFP713-P2A-GFP

Use

AAV

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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Sema3c(L)-AP-His

Plasmid

#72015

Purpose

Expresses the Sema3C protein (truncated at cleavage site P3; ie, long), C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema3c (Sema3c Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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3521 pET15b Bid p15

Plasmid

#8782

Depositor

Stanley Korsmeyer

Article

Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

Insert

Bid p15 (Bid Mouse)

Tags

His

Expression

Bacterial

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
px459-Hira KO sgRNA

Plasmid

#186938

Purpose

Hira KO in mouse ES cells

Depositor

Zhiguo Zhang

Article

Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

Insert

Hira KO sgRNA (Hira Mouse)

Use

CRISPR and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 8, 2022

Availability

Academic Institutions and Nonprofits only

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8024 GFP_Kras_G12C_2A_puro

Plasmid

#64373

Purpose

This a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2A oncogene along with puro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Insert

mouse Kras2A (Kras Mouse)

Use

Retroviral

Tags

GFP

Mutation

changed glycine 12 to cysteine

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pET28-Mff(1-61)-PP-GST

Plasmid

#73042

Purpose

Bacterial expression of mouse Mff residues 1-61 with C-terminal GST-tag and PreScission protease cleavage site

Depositor

David Chan

Article

Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77

Insert

Mff(1-61) (Mff Mouse)

Tags

GST-tag and PreScission protease site

Expression

Bacterial

Mutation

Truncation containing amino acids 1-61

Promoter

T7

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pMXs-IP-EGFP-ULK1(K46N)

Plasmid

#38197

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

changed Lysine 46 to Asparagine

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
pBa-eGFP-flag-BicD2 594-FKBP

Plasmid

#63569

Purpose

Bicaudal D protein aa1-594 (excluding start Met) tagged with N-terminal eGFP and flag and a C-terminal FKBP

Depositor

Gary Banker, Marvin Bentley

Article

Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

Insert

Bicaudal D protein (Bicd2 Mouse)

Tags

FKBP, eGFP, and flag

Expression

Mammalian

Mutation

no start methionine, aa1-594

Promoter

chicken Beta Actin

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-DiO-eGFP-2A-mCherry_D4H

Plasmid

#194880

Purpose

Ratiometric D4H sensor for in vivo cre-dependent neuron-specific cholesterol visualization

Depositor

Peter Scheiffele

Article

Czernecki et al bioRxiv 2022.11.16.516849

Insert

GFP-T2A-mCherry-D4H

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

human Synapsin

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST40-mPTER

Plasmid

#224864

Purpose

Mammalian expression mouse PTER protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

mouse PTER (Pter Mouse)

Tags

C-term v5 and His tag

Expression

Mammalian

Available Since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET20b-mPTER

Plasmid

#222189

Purpose

Bacterial expression of mouse PTER protein

Depositor

Jonathan Long

Article

Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

Insert

PTER (Pter Mouse)

Tags

6xHis and Strep

Expression

Bacterial

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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mEmerald-mDia2-C-14

Plasmid

#54158

Purpose

Localization: Cytoskeleton, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

mDia2

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pSV40_HA-mPKR

Plasmid

#120538

Purpose

protein expression

Depositor

Petr Svoboda

Insert

PKR (Eif2ak2 Mouse)

Expression

Mammalian

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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Tet-O-FUW-Olig2

Plasmid

#45844

Depositor

Marius Wernig

Article

Yang et al Nat Biotechnol. 2013 May;31(5):434-9. do

Insert

Olig2 (Olig2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tet-ON

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2.sgNf1.4

Plasmid

#92029

Purpose

sgNf1.4 for Nf1 deletion

Depositor

Wen Xue

Article

Song et al Gastroenterology. 2017 Apr;152(5):1161-1

Insert

sgNf1.4 for Nf1 deletion

Use

Lentiviral and Mouse Targeting

Expression

Mammalian

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pDendra2-H3

Plasmid

#157814

Purpose

Fluorescent reporter for histone H3 tracking

Depositor

Xin Chen

Article

Ma et al Cell Rep. 2020 Aug 11;32(6):108003. doi:

Insert

H3 (H3c1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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