We narrowed to 44,084 results for: cha

1. NAX_CAGGS_CAS9_m4_EBFP2

   Plasmid

   #167865

   Purpose

   PiggyBac compatible plasmid expressing dCas9

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. NAX_CAGGS_CAS9_iRFP713

   Plasmid

   #167860

   Purpose

   PiggyBac compatible plasmid expressing spCas9

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCDF-pntAB

   Plasmid

   #158609

   Purpose

   Low phosphate induction of pntAB

   Depositor

   Michael Lynch

   Article

   Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

   Insert

   pntAB(membrane bound proton translocating pyridine nucleotide transhydrogenase)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Insulated ugpBp from E. coli

   Available Since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCS105

   Plasmid

   #174830

   Purpose

   Encodes a unique yeast telomere end at TEL11R by removing the subtelomere

   Depositor

   Carol Greider

   Article

   Sholes et al Genome Res. 2022 Apr;32(4):616-628. doi:

   Insert

   11R Unique
   

   Use

   Tags

   GAL4 5XUAS

   Expression

   Bacterial

   Mutation

   Changing TEL11R to a unique end

   Promoter

   lac promoter

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. L1-Ck1-hs2-l-chlo

   Plasmid

   #163938

   Purpose

   For integration in the chloroplast rbcL_trnR region: HS2-L, genome position: 56139_57662, Genbank accession no. MH635409

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   Marchantia polymorpha rbcL_trnR region homologous sequence
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. PROM5_Mprnpo_chlo

   Plasmid

   #163951

   Purpose

   L0 part - promoter

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   Marchantia polymorpha chloroplast DNA-dependent RNA polymerase operon promoter (rpo) (genome position: 5595-5739)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pET29b-AviTag-mEGFP-dspB(E184Q)-6xHis

   Plasmid

   #176573

   Purpose

   Plasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

   Insert

   Dispersin B
   

   Use

   Tags

   Avitag, Hexahistidine tag, and mEGFP

   Expression

   Bacterial

   Mutation

   E184Q

   Promoter

   T7

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET29b-mVenus-dspB(E184Q W330Y)-6xHis

   Plasmid

   #176577

   Purpose

   Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.

   Depositor

   Mark Nitz

   Article

   Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

   Insert

   Dispersin B
   

   Use

   Tags

   Hexahistidine tag and mVenus

   Expression

   Bacterial

   Mutation

   E184Q W330Y

   Promoter

   T7

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FKBP-EGFP-PML peptide

   Plasmid

   #175247

   Purpose

   Bacterial expression and purification, low affinity SUMOylation substrate with a relatively high Km, can be recruited to FRB with rapamycin

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   PML (PML Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GST-OPTNS177DS473D

    Plasmid

    #171425

    Purpose

    For the purifcation of phosphorylation mimic protein

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    OPTN (OPTN Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    phosphorylation mutant (S177D, S473D)

    Promoter

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GST-TEVcs- FIP200 delta641-779-MBP

    Plasmid

    #171411

    Purpose

    For the purifcation of human ULK1 truncated complex

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    FIP200 (RB1CC1 Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    truncation (delta641-779-MBP)

    Promoter

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Dcp2C Δ5H-H1

    Plasmid

    #163584

    Purpose

    express Dcp2 FL-H1 at endogenous level in S.cerevisiae

    Depositor

    Michael Rosen

    Article

    Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

    Insert

    Dcp2 (DCP2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Truncation of aa1-326; the first HLM (EDQLKSYAEEQ…

    Promoter

    DCP2 promoter

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Dcp2 300 AAAA

    Plasmid

    #163579

    Purpose

    express Dcp2 300 AAAA at endogenous level in S.cerevisiae

    Depositor

    Michael Rosen

    Article

    Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

    Insert

    Dcp2 (DCP2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Truncation of C-terminal domain (301-970); mutati…

    Promoter

    DCP2 promoter

    Available Since

    April 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Dcp2 300 ΔH1

    Plasmid

    #163578

    Purpose

    express Dcp2 300 ΔH1 at endogenous level in S.cerevisiae

    Depositor

    Michael Rosen

    Article

    Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

    Insert

    Dcp2 (DCP2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Truncation of C-terminal domain (301-970); Lysine…

    Promoter

    DCP2 promoter

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-rsAKARev(T/A)

    Plasmid

    #166232

    Purpose

    Expresses reversibly switchable A-kinase activity reporter with EV-linker inactive T/A mutant (rsAKARev(T/A)) in mammalian cells

    Depositor

    Peter Dedecker

    Article

    Roebroek et al Nat Commun. 2021 Mar 31;12(1):2005. doi:

    Insert

    Reversibly switchable A-kinase activity reporter with EV-linker inactive T/A mutant
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Threonine 506 in rsAKARev to Alanine

    Promoter

    CMV

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. THI VP64 pALD4 T4

    Plasmid

    #161485

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pALD4_THI11_T4_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (targeting T4 of pTHI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pALD4, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. THI VP64 pALD4 T5

    Plasmid

    #161486

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pALD4_THI11_T5_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (targeting T5 of pTHI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pALD4, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. THI VP64 pGAP T2

    Plasmid

    #161489

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T2_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting T2 in the THI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. THI VP64 pGAP T3

    Plasmid

    #161490

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T3_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting T3 in the THI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. THI VP64 pGAP T4

    Plasmid

    #161491

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T4_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting T4 in the THI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. RIB VP64 R2

    Plasmid

    #161494

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R2_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting R2 in the RIB1 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. RIB VP64 R3

    Plasmid

    #161495

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R3_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting R3 in the RIB1 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. RIB VP64 R4

    Plasmid

    #161496

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R4_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting R4 in the RIB1 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pGAP, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. THI VP64 pALD4 T1

    Plasmid

    #161482

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pALD4_THI11_T1_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (targeting T1 of pTHI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pALD4, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. THI VP64 pALD4 T2

    Plasmid

    #161483

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pALD4_THI11_T2_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (targeting T2 of pTHI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pALD4, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. THI VP64 pALD4 T3

    Plasmid

    #161484

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pALD4_THI11_T3_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (targeting T3 of pTHI11 promoter)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pALD4, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET28a-ybbR-His-ELP(MV7E2)3-ddFLN4-SpyCatcher

    Plasmid

    #157674

    Purpose

    Expression of SpyCatcher with ddFLN4, ELP linker, His6x tag, and ybbR Tag.

    Depositor

    Michael Nash

    Article

    Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

    Insert

    SpyCatcher with ddFLN4, ELP, Histag and ybbR tag
    

    Use

    Tags

    ddFLN4, ELP, Histag and ybbR tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pQE80L-His-SpyTag-XMod(XL2V)-Doc

    Plasmid

    #157676

    Purpose

    Expression of XMod-Doc mutant XL2V (All LEUs were mutated to VAL in XMod region) with His6x tag and SpyTag.

    Depositor

    Michael Nash

    Article

    Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

    Insert

    XMod-Doc mutant XL2V with Histag and SpyTag
    

    Use

    Tags

    Histag and SpyTag

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAGM9121_Cgl-UPO SP

    Plasmid

    #153501

    Purpose

    Chaetomium globosum Unspecific Peroxygenase Signal Peptide Donor Plasmid

    Depositor

    Sylvestre Marillonnet

    Article

    Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

    Insert

    Unspecific peroxygenase SP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. BII-C3H

    Plasmid

    #133386

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. BII-B3HTK

    Plasmid

    #133389

    Purpose

    Piggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)

    Depositor

    Blair Madison

    Article

    Wen et al J Biol Chem. 2017 Apr 14;292(15):6148-61

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. EGFP-miSFIT-12-C

    Plasmid

    #124693

    Purpose

    Tuning expression of EGFP

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. EGFP-miSFIT-9-G

    Plasmid

    #124692

    Purpose

    Tuning expression of EGFP

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. EGFP-miSFIT-6-C,16-C

    Plasmid

    #124694

    Purpose

    Tuning expression of EGFP

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. EGFP-miSFIT-10-G, 15-C

    Plasmid

    #124695

    Purpose

    Tuning expression of EGFP

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBac(3xP3-gTc’v; Valcyrie.LR)

    Plasmid

    #119066

    Purpose

    Valcyrie construct for CRISPR-mediated lox excision in Tribolium castaneum (high)

    Depositor

    Michalis Averof

    Article

    Gilles et al Development. 2019 Jan 4;146(1). pii: dev

    Inserts

    pBacL
    Tc-U6a-Lox.L
    Tc-hsp68 Promoter
    NLS-Cas9
    Tc-hsp68 3'UTR
    Tc-U6B-Lox.R
    SV40polyA
    Tc-vermilion
    3xP3 TATA
    pBacR
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Spt2-K166R-GFP

    Plasmid

    #115573

    Purpose

    Expresses yeast Spt2-GFP fusion protein mutated from lysine to arginine at site 166

    Depositor

    Michael Downey

    Article

    Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62

    Insert

    SPT2
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Lysine 166 mutated to Arginine

    Promoter

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

38. DeltaNIRD C95S KVS-1

    Plasmid

    #113068

    Purpose

    Expresses worm truncated KVS1.2 C113S in mammalian cells

    Depositor

    Federico Sesti

    Article

    Cai et al Nat Neurosci. 2009 May;12(5):611-7. doi:

    Insert

    C113S K+ voltage sensitive channel subunit 1.2 (kvs-1 Nematode)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C113S+first 40 AA deletion

    Promoter

    Available Since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTH861-gst-optec P210T

    Plasmid

    #89595

    Purpose

    Plasmid for bacterial expression of a His-tagged P210T mutant of GST encoded by preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    glutathione-S transferase P210T mutant, codon-optimised for bacterial expression
    

    Use

    Tags

    8xhistidine

    Expression

    Bacterial

    Mutation

    changed proline 210 to threonine

    Promoter

    T7

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTH860-gst-optec A180Q

    Plasmid

    #89594

    Purpose

    Plasmid for bacterial expression of a His-tagged A180Q mutant of GST encoded by preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    glutathione-S transferase A180Q mutant, codon-optimised for bacterial expression
    

    Use

    Tags

    8xhistidine

    Expression

    Bacterial

    Mutation

    changed alanine 180 to glutamine

    Promoter

    T7

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pTH858-gst-optec V156E

    Plasmid

    #89592

    Purpose

    Plasmid for bacterial expression of a His-tagged V156E mutant of GST encoded by preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    glutathione-S transferase V156E mutant, codon-optimised for bacterial expression
    

    Use

    Tags

    8xhistidine

    Expression

    Bacterial

    Mutation

    changed valine 156 to glutamic acid

    Promoter

    T7

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pTH859-gst-optec Y172M

    Plasmid

    #89593

    Purpose

    Plasmid for bacterial expression of a His-tagged Y172M mutant of GST encoded by preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    glutathione-S transferase Y172M mutant, codon-optimised for bacterial expression
    

    Use

    Tags

    8xhistidine

    Expression

    Bacterial

    Mutation

    changed tyrosine 172 to methionine

    Promoter

    T7

    Available Since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. TU#1412_tiam-1_cDNA

    Plasmid

    #82364

    Purpose

    express tiam-1 cDNA

    Depositor

    Martin Chalfie

    Article

    Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    tiam-1 cDNA (tiam-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    none

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pHU6_Ch5_155183064-gRNA-rev

    Plasmid

    #81215

    Purpose

    pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of pCALNL_Ch5 reporter

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    hU6 expression of gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pHU6_gRNA_Ch10-for2

    Plasmid

    #81209

    Purpose

    for targeting Ch10 psuedo gix site in PCDH15 gene (five base pairs from the cas9 site and 3' of gix site)

    Depositor

    David Liu

    Article

    Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

    Insert

    hU6 expression of gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. TU#1449_unc-73e_cDNA

    Plasmid

    #82363

    Purpose

    express unc-73e cDNA

    Depositor

    Martin Chalfie

    Article

    Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    unc-73 e isoform (unc-73 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    none

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

47. pMpGWB434

    Plasmid

    #68699

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Glucocorticoid receptor (GR)

    Expression

    Plant

    Mutation

    Promoter

    MpHSP17.8A1 promoter

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pMpGWB433

    Plasmid

    #68698

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Citrine

    Expression

    Plant

    Mutation

    Promoter

    MpHSP17.8A1 promoter

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pMpGWB410

    Plasmid

    #68675

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    3xFLAG

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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50. pMpGWB413

    Plasmid

    #68678

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Glucocorticoid receptor (GR)

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge