We narrowed to 11,278 results for: VARS

1. pZS157 CRISPEY RT/Cas9

   Plasmid

   #114454

   Purpose

   Yeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expression

   Depositor

   Hunter Fraser

   Article

   Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

   Insert

   SpCas9, Ec86-RT
   

   Use

   CRISPR

   Tags

   SV40-NLS

   Expression

   Yeast

   Mutation

   S. cerevisiae codon optimized

   Promoter

   GAL1-GAL10

   Available Since

   Oct. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCLXEBR-pTF-hM3Dq

   Plasmid

   #114283

   Purpose

   TET-inducible expression of the hM3Dq protein

   Depositor

   Patrick Salmon

   Article

   Bocchi et al Nat Commun. 2017 Oct 27;8(1):1158. doi:

   Insert

   hM3Dq
   

   Use

   Lentiviral

   Available Since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-S in pAR9 (rep/cap)

   Plasmid

   #174539

   Purpose

   encodes AAV-S capsid for efficient gene delivery to the mammalian cochlea

   Depositor

   Casey Maguire

   Article

   Ivanchenko et al Mol Ther Methods Clin Dev. 2021 Mar 29;2

   Insert

   VP1 of AAV9 containing the AAV-S 7-mer peptide insert
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. AD59_pEP.DonorCLYBL.TS

   Plasmid

   #199227

   Purpose

   Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

   Insert

   Expression unit for PuroR.T2A.EGFP selectable and reporter markers
   

   Use

   Gene targeting donor plasmid

   Expression

   Mammalian

   Promoter

   Human PGK1 gene promoter

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEThT-CAHS3

   Plasmid

   #90028

   Purpose

   To express His-tagged CAHS3 in bacteria

   Depositor

   Takekazu Kunieda

   Article

   Yamaguchi et al PLoS One. 2012;7(8):e44209.

   Insert

   CAHS3
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5-hGRK4

   Plasmid

   #32690

   Depositor

   Robert Lefkowitz

   Article

   Premont et al J Biol Chem. 1996 Mar 15;271(11):6403-10

   Insert

   GRK4 (GRK4 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

7. GFP-PELP1

   Plasmid

   #65208

   Purpose

   Full length mammalian expression vector for hPELP1

   Depositor

   Ratna Vadlamudi

   Article

   Nagpal et al Mol Cancer Res. 2008 May;6(5):851-61. do

   Insert

   PELP1 (PELP1 Human)
   

   Tags

   AcGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

8. Luc-noPS

   Plasmid

   #177940

   Purpose

   Transient mammalian expression of Luc2 (firefly luciferase). Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   Luc2
   

   Expression

   Mammalian

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO.1 puro_shTBK1_3185

   Plasmid

   #167294

   Purpose

   Lentiviral plKO.1 construct coding for a short-hairpin RNA targeting the human gene TBK1. Contains a puro resistance cassette.

   Depositor

   Agnel Sfeir

   Article

   Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

   Insert

   shRNA TBK1 (TBK1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-GFP-Flag-UTR

    Plasmid

    #183235

    Purpose

    To express FLAG tagged eGFP

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Insert

    EGFP
    

    Tags

    FLAG tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3xFLAG-SENP7

    Plasmid

    #42886

    Depositor

    Edward Yeh

    Article

    Bawa-Khalfe et al Proc Natl Acad Sci U S A. 2012 Oct 23;10

    Insert

    SENP7 (SENP7 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    T102A, R969C, L996S

    Available Since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. MS2_adRNA-MCP_ADAR1 DD (E1008Q)_NES

    Plasmid

    #124703

    Purpose

    Expresses MS2_adRNA targeting the RAB7A locus along with MCP_ADAR1 DD (E1008Q)_NES

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

    Insert

    MCP_ADAR2 DD (E1008Q)_NES
    

    Use

    AAV

    Tags

    NES

    Expression

    Mammalian

    Mutation

    E1008Q hyperactive mutant

    Promoter

    CMV

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJE53

    Plasmid

    #194153

    Purpose

    sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, KmR. Contains mrfp nontargeting control guide sequence.

    Depositor

    Edward Geisinger

    Article

    Bai et al J Bacteriol. 2021 May 20;203(12):e005652

    Insert

    sgRNA with mrfp control guide sequence
    

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSMART-sgRNA (Sp)

    Plasmid

    #80427

    Purpose

    U6 promoter driven sgRNA expression plasmid. Annealed oligonucleotides encoding crRNA sequence are ligated into BbsI site.

    Depositor

    Sara Howden, Melissa Little

    Article

    Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. Thy1 promoter construct

    Plasmid

    #20736

    Depositor

    Joshua Sanes

    Article

    Feng et al Neuron. 2000 Oct . 28(1):41-51.

    Insert

    Thy1 promoter (Thy1 Mouse)
    

    Expression

    Mammalian

    Available Since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. p3xFLAG-MKL1

    Plasmid

    #11978

    Purpose

    For mammalian expression of human MKL1 with a 3x FLAG tag

    Depositor

    Ron Prywes

    Article

    Cen et al Mol Cell Biol. 2003 Sep . 23(18):6597-60

    Insert

    MKL1 (MRTFA Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    N579S T716A

    Promoter

    CMV

    Available Since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. PRS_8-GCaMP8m

    Plasmid

    #210400

    Purpose

    AAV-vector for fluorescent calcium measurements in the green spectrum from noradrenergic neurons

    Depositor

    Simon Wiegert

    Article

    Wissing et al PLoS Biol. 2025 Jul 7;23(7):e3003228. do

    Insert

    GCaMP8m
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    PRSx8

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. gfp-Arkadia/RNF111

    Plasmid

    #112228

    Purpose

    Expresses GFP-tagged Arkadia in mammalian cells

    Depositor

    Vasso Episkopou

    Article

    Mavrakis et al PLoS Biol. 2007 Mar;5(3):e67. doi: 10.13

    Insert

    (Arkadia)Rnf111 (Rnf111 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. MC177VSG221RNAi

    Plasmid

    #59730

    Purpose

    For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.

    Depositor

    Gloria Rudenko

    Article

    Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10

    Insert

    VSG221 RNAi fragment
    

    Use

    RNAi

    Promoter

    opposing T7 promoters

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. BPK1520_puroR

    Plasmid

    #173901

    Purpose

    SpCas9 guide cloning vector derived from BPK1520 that allows for puromycin-based selection

    Depositor

    Ronald Cohn

    Article

    Erwood et al Genome Res. 2019 Dec;29(12):2010-2019. d

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV/U6

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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