We narrowed to 34,448 results for: CaS

1. pEN_TT 3xFLAG-MAVS-Ala271

   Plasmid

   #158631

   Purpose

   Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   MAVS (MAVS Human)
   

   Use

   Gateway entry vector

   Tags

   3XFLAG

   Expression

   Mutation

   Q271A please see depositor comments below

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEN_TT 3xFLAG-MAVS-Gln93

   Plasmid

   #158629

   Purpose

   Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   MAVS (MAVS Human)
   

   Use

   Gateway entry vector

   Tags

   3XFLAG

   Expression

   Mutation

   E93Q

   Promoter

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSLIK 3xFLAG-MAVS-Ala271 hygro

   Plasmid

   #158637

   Purpose

   Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   MAVS (MAVS Human)
   

   Use

   Lentiviral

   Tags

   3XFLAG

   Expression

   Mutation

   Q271A

   Promoter

   Available since

   Nov. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSLIK 3xFLAG-MAVS-Gln93,Ala271 hygro

   Plasmid

   #158638

   Purpose

   Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   MAVS (MAVS Human)
   

   Use

   Lentiviral

   Tags

   3XFLAG

   Expression

   Mutation

   E93Q/Q271A

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEN_TT 3xFLAG-MAVS-Gln93,Ala271

   Plasmid

   #158632

   Purpose

   Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   MAVS (MAVS Human)
   

   Use

   Gateway entry vector

   Tags

   3XFLAG

   Expression

   Mutation

   E93Q/Q271A

   Promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEN_TT 3xFLAG-MAVS-Ala148,Ala271

   Plasmid

   #158633

   Purpose

   Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   MAVS (MAVS Human)
   

   Use

   Gateway entry vector

   Tags

   3XFLAG

   Expression

   Mutation

   Q148A/Q271A

   Promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSLIK 3xFLAG-MAVS-Ala148,Ala271 hygro

   Plasmid

   #158639

   Purpose

   Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   MAVS (MAVS Human)
   

   Use

   Lentiviral

   Tags

   3XFLAG

   Expression

   Mutation

   Q148A/Q271A

   Promoter

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEN_TT 3xFLAG-MAVS-Ala148

   Plasmid

   #158630

   Purpose

   Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   MAVS (MAVS Human)
   

   Use

   Gateway entry vector

   Tags

   3XFLAG

   Expression

   Mutation

   Q148A

   Promoter

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSLIK 3xFLAG-MAVS-Ala148 hygro

   Plasmid

   #158636

   Purpose

   Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   MAVS (MAVS Human)
   

   Use

   Lentiviral

   Tags

   3XFLAG

   Expression

   Mutation

   Q148A

   Promoter

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSLIK 3xFLAG-MAVS-Gln93 hygro

    Plasmid

    #158635

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    MAVS (MAVS Human)
    

    Use

    Lentiviral

    Tags

    3XFLAG

    Expression

    Mutation

    E93Q

    Promoter

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. YCplac33-NAT_MCART1K91A

    Plasmid

    #140592

    Purpose

    Express MCART1K91A in S. cerevisiae

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    SLC25A51 with 5' and 3' UTR of scNDT1 (SLC25A51 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    codon-optimized for expression in S. cerevisiae; …

    Promoter

    NDT1 promoter and 3'UTR

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCEP4-myc-H2-Ld-VN

    Plasmid

    #135520

    Purpose

    Mammalian expression of VN-fused and myc-tagged H2-Ld

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    H2-Ld
    

    Use

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ID1GFP Donor

    Plasmid

    #119282

    Purpose

    Mouse ID1-eGFP for HR to create reporter

    Depositor

    Joan Massague

    Article

    Huang et al Cancer Discov. 2019 Oct 3. pii: 2159-829

    Insert

    Id1GFP + homology arms (Id1 Mouse)
    

    Use

    Reporter

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJET1.2[eb-Tc’bhsp-EGFP-2A-Cre;3xP3-gTc’v-SV40]

    Plasmid

    #124068

    Purpose

    CRISPR/Cas mediated knock-in via non-homologous end-joining in Tribolium; bhsp drives EGFP expression; Dm-ebony for linearization; (Transformation plasmid; Black eye marker)

    Depositor

    Gregor Bucher

    Article

    He et al Elife. 2019 Oct 18;8. pii: 49065. doi: 1

    Insert

    eb-Tc’bhsp-EGFP-2A-Cre; 3xP3-gTc’v-SV40
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Insect

    Mutation

    Promoter

    bhsp68

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMXs_FLAG-Sfxn1-3 (CG11739)

    Plasmid

    #110642

    Purpose

    Retroviral expression of Flag-tagged Drosophila CG11739 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    CG11739 (CG11739 Fly)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon-optimized cDNA (no amino acid mutations)

    Promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJC20cdc8.27

    Plasmid

    #99362

    Purpose

    Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.27.

    Depositor

    Dan Mulvihill

    Article

    Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

    Insert

    cdc8 (cdc8 Fission Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Glutamic acid 129 to Lysine

    Promoter

    T7

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA3-HA/Bmal F423R/V435R/W427R

    Plasmid

    #47351

    Purpose

    PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Ha

    Expression

    Mammalian

    Mutation

    F423R/V435R/W427R

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

18. HLH Clock Pas 1-401 VenN L113E

    Plasmid

    #47356

    Purpose

    Clock fragment mutatation tagged with VenN used for BiFC

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    Venus aa 1-155

    Expression

    Mammalian

    Mutation

    aa 1-401, L113E

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

19. HLH Clock Pas 1-401 VenN F122D

    Plasmid

    #47357

    Purpose

    Clock fragment mutatation tagged with VenN used for BiFC

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    Venus aa 1-155

    Expression

    Mammalian

    Mutation

    aa 1-401, F122D

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

20. HLH Clock Pas 1-401 VenN W284A

    Plasmid

    #47358

    Purpose

    Clock fragment mutatation tagged with VenN used for BiFC

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    Venus aa 1-155

    Expression

    Mammalian

    Mutation

    aa 1-401, W284A

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

21. HLH Clock Pas 1-401 VenN V315R

    Plasmid

    #47359

    Purpose

    Clock fragment mutatation tagged with VenN used for BiFC

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    Venus aa 1-155

    Expression

    Mammalian

    Mutation

    aa 1-401, V315R

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

22. HLH Clock Pas 1-401 VenN L57E/L113E/W284A

    Plasmid

    #47360

    Purpose

    Clock fragment mutatation tagged with VenN used for BiFC

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    Venus aa 1-155

    Expression

    Mammalian

    Mutation

    aa 1-401, L57E/L113E/W284A

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

23. HLH Bmal Pas 1-465-VenC L95E

    Plasmid

    #47365

    Purpose

    Bmal fragment cloned with Venus tag used for mutation

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Venus aa 156-239

    Expression

    Mammalian

    Mutation

    aa 1-465, L95E

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

24. HLH Bmal Pas 1-465-VenC L150E

    Plasmid

    #47366

    Purpose

    Bmal fragment cloned with Venus tag used for mutation

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Venus aa 156-239

    Expression

    Mammalian

    Mutation

    aa 1-465, L150E

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

25. HLH Bmal Pas 1-465-VenC L95E/L150E/W427A

    Plasmid

    #47372

    Purpose

    Bmal fragment cloned with Venus tag used for mutation

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Venus aa 156-239

    Expression

    Mammalian

    Mutation

    aa 1-465, L95E/L150E/W427A

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

26. HLH Bmal Pas 1-465-VenC L115E

    Plasmid

    #47377

    Purpose

    Bmal fragment cloned with Venus tag used for mutation

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Venus aa 156-239

    Expression

    Mammalian

    Mutation

    L115E

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

27. HLH Bmal Pas 1-465-VenC F423R

    Plasmid

    #47368

    Purpose

    Bmal fragment cloned with Venus tag used for mutation

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Venus aa 156-239

    Expression

    Mammalian

    Mutation

    aa 1-465, F423R

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

28. HLH Bmal Pas 1-465-VenC W427A

    Plasmid

    #47369

    Purpose

    Bmal fragment cloned with Venus tag used for mutation

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Venus aa 156-239

    Expression

    Mammalian

    Mutation

    aa 1-465, W427A

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

29. HLH Bmal Pas 1-465-VenC V435R

    Plasmid

    #47370

    Purpose

    Bmal fragment cloned with Venus tag used for mutation

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Venus aa 156-239

    Expression

    Mammalian

    Mutation

    aa 1-465, V435R

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

30. HLH Bmal Pas 1-465-VenC F423R/V435R

    Plasmid

    #47371

    Purpose

    Bmal fragment cloned with Venus tag used for mutation

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    BMAL (Bmal1 Mouse)
    

    Use

    Tags

    Venus aa 156-239

    Expression

    Mammalian

    Mutation

    aa 1-465, F423R/V435R

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

31. HLH Clock Pas 1-401 VenN L57E

    Plasmid

    #47354

    Purpose

    Clock fragment mutatation tagged with VenN

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    Venus aa 1-155

    Expression

    Mammalian

    Mutation

    aa 1-401, L57E

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

32. FNE2-A72

    Plasmid

    #44608

    Depositor

    Robert Oshima

    Article

    Galang et al J Biol Chem. 1996 Apr 5;271(14):7992-8.

    Insert

    Ets2-A72 (Ets2 Mouse)
    

    Use

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    THR-72 converted to Alanine

    Promoter

    CMV

    Available since

    April 26, 2013

    Availability

    Academic Institutions and Nonprofits only

33. pEMS1241

    Plasmid

    #29123

    Purpose

    Expression of the eGFP reporter gene was not detected in the brain and eye.

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple12
    

    Use

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEMS1207

    Plasmid

    #29104

    Purpose

    Expression of the eGFP reporter gene was not detected in the brain and eye.

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple235
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET28a-MED20

    Plasmid

    #15435

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED20 (Med20 Mouse)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    Available since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

36. Kv7.4 AD (325-645, C643A, Δ 368-492)/pET28HMT

    Plasmid

    #111072

    Purpose

    Protein expression in E.Coli

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    His Tag and MBP tag

    Expression

    Bacterial

    Mutation

    Kv7.4 AD (325-645, C643A, Δ 368-492)

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Kv7.4 BD (522-645, C643A)/pET28HMT

    Plasmid

    #111073

    Purpose

    Protein expression in E.Coli

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    His Tag and MBP tag

    Expression

    Bacterial

    Mutation

    Kv7.4 BD (522-645, C643A)

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Kv7.5 AB (361-545, Δ395-511)/pET28HMT

    Plasmid

    #111267

    Purpose

    Protein expression in E.Coli

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 5 isoform 1 [Homo sapiens] (KCNQ5 Human)
    

    Use

    Tags

    His Tag and MBP Tag

    Expression

    Bacterial

    Mutation

    Kv7.5 AB (361-545, Δ395-511)

    Promoter

    T7

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. Kv7.4 (1-645, Δ368-492)/pcDNA3.1

    Plasmid

    #111454

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Kv7.4 (1-645, Δ368-492), C643A

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Kv7.4 (1-645, Δ368-523)/I346A/HA tag/pcDNA3.1

    Plasmid

    #111460

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    Ha Tag

    Expression

    Mammalian

    Mutation

    Kv7.4 (1-645, Δ368-523)/I346A, C643A

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. Kv7.4 (1-645, Δ368-523)/I346D/HA tag/pcDNA3.1

    Plasmid

    #111461

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    Ha Tag

    Expression

    Mammalian

    Mutation

    Kv7.4 (1-645, Δ368-523)/I346D, C643A

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCMV-T7-hOrf2mor-NLS(sv40) (BPK5095)

    Plasmid

    #115141

    Purpose

    CMV-T7 promoter expression plasmid for human codon optimized Orf2mor anti-CRISPR protein with C-terminal NLS

    Depositor

    Benjamin Kleinstiver, Keith Joung, Joseph Bondy-Denomy

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized Orf2mor anti-CRISPR protein
    

    Use

    Tags

    NLS(SV40)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pMXs_FLAG-MCART1R278A

    Plasmid

    #138408

    Purpose

    Express FLAG-tagged MCART1R278A in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    SLC25A51 (SLC25A51 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells, R2…

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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44. pLenti-sgRNA

    Plasmid

    #71409

    Purpose

    There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI

    Depositor

    Eric Lander, David Sabatini

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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45. pCFD5_w

    Plasmid

    #112645

    Purpose

    Ubiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.

    Depositor

    Michael Boutros

    Insert

    U6:3-tRNA-sgRNA expression cassette
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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46. pWS082

    Plasmid

    #90516

    Purpose

    sgRNA entry vector

    Depositor

    Tom Ellis

    Insert

    Yeast sgRNA expression vector
    

    Use

    CRISPR; Yeast gap repair

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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47. BPK1520_blastR

    Plasmid

    #175289

    Purpose

    Human expression plasmid for SpCas9 sgRNA with blasticidin co-selection

    Depositor

    Ronald Cohn

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/U6

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. pWS173

    Plasmid

    #90960

    Purpose

    Cas9 gap repair vector - KanR

    Depositor

    Tom Ellis

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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49. pY116 (pcDNA3.1-huMb2Cpf1)

    Plasmid

    #92292

    Purpose

    Expression of humanized Mb2Cpf1

    Depositor

    Feng Zhang

    Insert

    huMb2Cpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. pBHM2644

    Plasmid

    #229528

    Purpose

    Carries AID* tag, empty sgRNA, and NATR marker

    Depositor

    Hiten Madhani

    Insert

    AID* - empty sgRNA - NATR
    

    Use

    Tags

    2xFLAG

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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