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I-SceI pB 5UAS::ZfSyGCaMP2

Plasmid

#26125

Depositor

Leon Lagnado

Article

Dreosti et al Nat Methods. 2009 Dec . 6(12):883-9.

Insert

Synaptophysin GCaMP2 (sypb Zebrafish)

Use

Zebrafish expression

Tags

GCaMP2

Available Since

Oct. 18, 2010

Availability

Academic Institutions and Nonprofits only

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Ddo_innexin-10

Plasmid

#107633

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

innexin-10

Use

RNAi; In situ hybridization

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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6920 pPROEXHsp104b

Plasmid

#1230

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

Hsp104 (HSP104 Budding Yeast)

Tags

his tag (tev cleavable)

Expression

Bacterial

Available Since

Aug. 15, 2005

Availability

Academic Institutions and Nonprofits only
p3692 HPV-16 E1

Plasmid

#10859

Depositor

Peter Howley

Article

Sakai et al J Virol. 1996 Mar . 70(3):1602-11.

Insert

HPV-16 E1 (E1 Human papillomavirus)

Expression

Mammalian

Available Since

Oct. 26, 2005

Availability

Academic Institutions and Nonprofits only

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pFUW mCherry

Plasmid

#239564

Purpose

Cytosolic expression of mCherry

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mCherry

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-FDD

Plasmid

#80629

Purpose

Fluorescent destablizing domain for regulating protein stability and fluorescence

Depositor

Thomas Wandless

Article

Navarro et al ACS Chem Biol. 2016 Jun 6.

Insert

UnaG

Use

Retroviral

Tags

HA-mcherry-linker

Mutation

mutations A36V, R136G

Promoter

LTR

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTK630

Plasmid

#114697

Purpose

Express RFP-Nano from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

RFP-Nano

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pTK96_mCherry-MRLC2

Plasmid

#46358

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

MRLC2 (MYL12B Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

pBABE

Available Since

July 31, 2013

Availability

Academic Institutions and Nonprofits only

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pCMVMagFRET-6

Plasmid

#50756

Purpose

Expresses MagFRET-6 in the cytosol of mammalian cells. Weak affinity control FRET sensor for MagFRET-1.

Depositor

Maarten Merkx

Article

Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

Insert

gene encoding for MagFRET-6

Expression

Mammalian

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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lvl0 lexA-35S

Plasmid

#153407

Purpose

Estradiol system inducible promoter

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

lexA-35S promoter

Expression

Plant

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-iPASnFR-L-mScarlet3-3xFLAG-Rab5

Plasmid

#225244

Purpose

Mammalian expression of iPASnFR-L fused to Rab5

Depositor

Loren Looger

Article

Tamura et al bioRxiv 2024.08.21.609037

Insert

iPASnFR-L

Tags

3xFLAG and mScarlet3

Expression

Mammalian

Available Since

Sept. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pTK750

Plasmid

#114718

Purpose

Express mCherry-NuMA(5A-3) from Rosa 26 locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

NuMA 5A-3

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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Ddo_opsin

Plasmid

#107630

Purpose

In situ hybridization and RNAi studies in planarians

Depositor

Alejandro Sánchez

Article

Accorsi et al The American Biology Teacher 79(3):208-2

Insert

opsin

Use

RNAi; In situ hybridization

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLJSRSF7

Plasmid

#64693

Purpose

His-MBP-Smt3-NpuDnaEN102-CBD

Depositor

Hideo Iwai

Article

Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

Type

Empty backbone

Tags

CBD, HisTag, MBP, NpuDnaE-N102, and Smt3

Expression

Bacterial

Promoter

T7

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti.PGK.H2B-chFP.W

Plasmid

#51007

Purpose

Lentivector that expresses H2B-chFP from internal human PGK promoter

Depositor

Rusty Lansford

Article

Huss et al Development. 2015 Jul 24. pii: dev.12139

Insert

H2B-ChFP

Use

Lentiviral

Tags

histone2B

Expression

Mammalian

Promoter

human PGK

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Myr-Pink Flamindo

Plasmid

#204669

Purpose

Mammalian expression of membrane-bound Pink Flamindo for cAMP imaging

Depositor

Adam Cohen

Article

Xiang et al bioRxiv 2023

Insert

Myr-Pink Flamindo

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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p060 pCS mTRPS1

Plasmid

#11032

Depositor

Ramesh Shivdasani

Article

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

TRPS1 (Trps1 Mouse)

Use

Xenopus, mammalian, avian, and zebrafish

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pU

Plasmid

#64180

Purpose

harbours marker gene URA3

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pTK586

Plasmid

#114716

Purpose

SNAP-tagging at LGN's N-terminal locus (BSD)

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

SNAP

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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YFP-PKCbetaII-YFP

Plasmid

#14866

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

YFP-PKCbetaII-YFP

Tags

YFP

Expression

Mammalian

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only
pJL1-FETCH1

Plasmid

#210644

Purpose

In vitro expression of FETCH reporter from the T7 promoter

Depositor

Michael Jewett

Article

Willi et al ACS Synth Biol. 2024 Jul 19;13(7):2253-2

Insert

FETCH1

Use

Synthetic Biology

Promoter

T7

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pWS3865

Plasmid

#185742

Purpose

Sub-array backbone 1/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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ZE31 Prickle-1-pCS2P+

Plasmid

#17072

Depositor

Randall Moon

Article

Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

Insert

Prickle-1 (prickle1a Zebrafish)

Use

Zebrafish expression

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pEXP5-NT/pLux eGFP

Plasmid

#193624

Purpose

Inducible expression of eGFP by the transcription factor complex LuxR-3OC6 homoserine lactone (HSL).

Depositor

Dora Tang

Article

Gonzales et al ChemSystemsChem 2023

Insert

eGFP

Tags

6xHis

Expression

Bacterial

Promoter

pLux

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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GNb-RNb

Plasmid

#128787

Purpose

Mammalian expression

Depositor

Colin Taylor

Article

Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

Insert

GNb-RNb

Expression

Mammalian

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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CKAR-T/A

Plasmid

#14861

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

C kinase activity reporter

Tags

CFP and YFP

Expression

Mammalian

Mutation

T/A

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pMLS134

Plasmid

#73714

Purpose

SapTrap destination vector for building sgRNA expression vectors.

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

pU6::sgRNA

Use

CRISPR

Expression

Worm

Mutation

SapI insertion site

Promoter

C. elegans U6 snRNA pol III promoter

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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6375 H10Ydj1

Plasmid

#1234

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

Ydj1 (YDJ1 Budding Yeast)

Tags

his tag

Expression

Bacterial

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pWS3866

Plasmid

#185743

Purpose

Sub-array backbone 2/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
ZP46 Prickle-2

Plasmid

#16896

Depositor

Randall Moon

Article

Veeman et al Curr Biol. 2003 Apr 15. 13(8):680-5.

Insert

Prickle-2 (prickle2b Zebrafish)

Expression

Bacterial

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only