We narrowed to 13,571 results for: GAN

1. pXPR_003 Intergenic control guide 2

   Plasmid

   #193585

   Purpose

   sgRNA control; induces CAS9 cutting in an intergenic region

   Depositor

   William Hahn

   Article

   Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

   Insert

   sgRNA control
   

   Use

   Lentiviral

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXPR_003 sgNFkB2 guide 2

   Plasmid

   #193594

   Purpose

   NFkB2 knockout

   Depositor

   William Hahn

   Article

   Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

   Insert

   sgNFkB2 guide 2 (NFKB2 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRKB134_GI-DUX4-intron2(-)

   Plasmid

   #83968

   Purpose

   to test DUX4 mRNA is subjected to NMD upon intron2 inclusion

   Depositor

   Robert Bradley

   Article

   Feng et al Elife. 2015 Jan 7;4. doi: 10.7554/eLife.

   Insert

   DUX4-3'UTR-intron2(-) (DUX4 Human)
   

   Expression

   Mammalian

   Promoter

   mouseCMV

   Available Since

   Jan. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

4. pRKB133_GI-DUX4-intron2(+)

   Plasmid

   #83967

   Purpose

   to test DUX4 mRNA is subjected to NMD upon intron2 inclusion

   Depositor

   Robert Bradley

   Article

   Feng et al Elife. 2015 Jan 7;4. doi: 10.7554/eLife.

   Insert

   DUX4-3'UTR-intron2(+) (DUX4 Human)
   

   Expression

   Mammalian

   Promoter

   mouseCMV

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3.1(+)-ER-BSCaM2

   Plasmid

   #64855

   Purpose

   Endoplasmic reticulum (ER)-targeted version of a high-affinity FRET-based biosensor for monitoring Ca2+/CaM levels; targeted to cytosolic face of ER membrane.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   CYP450-BSCaM2
   

   Tags

   CFP, N-terminal 27 amino acids from CYP450, and Y…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. PhyB-mCherry-Htb2

   Plasmid

   #51569

   Purpose

   Constitutively express nuclear localized PhyB in Saccharomyces cerevisiae

   Depositor

   Chao Tang

   Article

   Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

   Insert

   PhyB (PHYB Mustard Weed)
   

   Tags

   HTB2 and mCherry

   Expression

   Yeast

   Mutation

   1-908aa only

   Promoter

   ADH1pr

   Available Since

   Aug. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. CFP-GAI(1-532)

   Plasmid

   #37310

   Depositor

   Takanari Inoue

   Article

   Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

   Insert

   GAI (GAI Mustard Weed)
   

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   contains aa 1-532

   Promoter

   CMV

   Available Since

   June 15, 2012

   Availability

   Academic Institutions and Nonprofits only

8. NLS-GAI(1-92)

   Plasmid

   #37317

   Depositor

   Takanari Inoue

   Article

   Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

   Insert

   GAI (GAI Mustard Weed)
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   contains aa 1-92

   Promoter

   CMV

   Available Since

   June 15, 2012

   Availability

   Academic Institutions and Nonprofits only

9. CSII-IDR2-owlTRIMCyp-FOS

   Plasmid

   #79067

   Purpose

   Express owl monkey TRIMCyp in mammalan cells

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   TRIM5Cyp (owl monkey)-FOS
   

   Tags

   OneSTrEP-FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-EF1a-V5-Sec61B

    Plasmid

    #120244

    Purpose

    Expresses V5-tagged Sec61B in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Sec61B (Sec61b Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Promoter

    EF1a

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGG184

    Plasmid

    #165604

    Purpose

    Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with a single hEGFP protospacer: PS1(upstream of promoter with 'CAGCG' PAM)-lac-HIS3 and GFP

    Depositor

    Marcus Noyes

    Article

    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

    Insert

    hEGFP protospacer ('CAGCG' PAM) upstream of the HIS3/GFP promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    'CAGCG' PAM replacing the 'CGGCG&#…

    Promoter

    lac

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. ZJOM141

    Plasmid

    #133669

    Purpose

    Integration of COX9-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast mitochondria.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-COX9-mTagBFP2 (COX9 Budding Yeast)
    

    Tags

    mTagBFP2

    Expression

    Yeast

    Available Since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJG363

    Plasmid

    #91183

    Purpose

    BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase (nAtCas9_D10A+gNt_R2+donor)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    nAtCas9_D10A+gNt_R2+donor
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJG370

    Plasmid

    #91187

    Purpose

    BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase no gRNA control (nAtCas9_D10A+gRNActrl+donor)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    nAtCas9_D10A+gRNActrl+donor
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+)-ExRai-AKAR-Kras

    Plasmid

    #118408

    Purpose

    Plasma membrane-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    ExRai-AKAR-Kras
    

    Tags

    C-terminal targeting sequence from Kras

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-MCS-13 x Linker-BioID2-HA-GPI-PSS

    Plasmid

    #194750

    Purpose

    To fuse your protein of interest to the N-terminus of GPI-anchored-BioID2

    Depositor

    Chaoyang Li

    Article

    Gao et al iScience. 2022 Nov 2;25(12):105481. doi:

    Type

    Empty backbone

    Tags

    HA

    Expression

    Mammalian

    Available Since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA_DiCre_59.F2

    Plasmid

    #60207

    Purpose

    Expresses a fusion protein of FKBP12-linker peptide 2- Cre(19-59)

    Depositor

    Jean-Paul Herman

    Article

    Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

    Insert

    Cre recombinase (CD207 Human, Bacteriophage P1, Synthetic)
    

    Tags

    FKBP12 and Nuclear localisation signal

    Expression

    Mammalian

    Mutation

    amino acids 19-59 of the humanized version of Cre…

    Promoter

    CMV

    Available Since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. S12.1_Empty_Bridge

    Plasmid

    #127670

    Purpose

    Ampicillin resistant multiplexing backbone without CRY2HA and mCherry

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-Nxph4-HA

    Plasmid

    #128102

    Purpose

    Express HA tagged Nxph4

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nxph4 (Nxph4 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMOD_C2813

    Plasmid

    #91092

    Purpose

    Module C, Promoter: PvUbi2, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4 (+ BaeI site for GT donor cloning)
    

    Use

    CRISPR

    Promoter

    PvUbi2

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. ZJOM2

    Plasmid

    #133633

    Purpose

    Integration of VRG4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast early golgi.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-VRG4-GFP (VRG4 Budding Yeast)
    

    Tags

    EGFP

    Expression

    Yeast

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSR29

    Plasmid

    #69255

    Purpose

    pelt-2::CRE for integration on ttTi14024, Chr X

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Insert

    Cre recombinase
    

    Use

    Cre/Lox; Mossci

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0, tbb-2 UTR is in wrong o…

    Promoter

    elt-2

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. MTK0_017

    Plasmid

    #123932

    Purpose

    Encodes the BxBI attB GFP dropout destination vector constitutive NLS::tagBFP as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    BxBI attB tagBFP destination vector
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFastBac1-OSF-TRIM5alpha(chimpanzee)

    Plasmid

    #79032

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(chimpanzee)
    

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pUSE-Src-KD-UniRapR-mCerulean-myc

    Plasmid

    #45382

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

    Insert

    Src-KD-UniRapR-mCerulean-myc
    

    Tags

    mCerulean and myc

    Expression

    Mammalian

    Mutation

    mSrc R388D (kinase dead), Y529F

    Promoter

    CMV

    Available Since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3-RAB-AKARev(T/A)

    Plasmid

    #118479

    Purpose

    Negative-control mutant for RAB-AKARev biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    RAB-AKARev(T/A)
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    Dec. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. NELFE-mOrange pHis-//

    Plasmid

    #53303

    Purpose

    Expresses His-tagged NELF-E-mOrange fusion protein.

    Depositor

    John Lis

    Article

    Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

    Insert

    NELF-E protein (Nelf-E Fly)
    

    Tags

    His and mOrange

    Expression

    Bacterial

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMOD_B2517

    Plasmid

    #91074

    Purpose

    Module B, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning
    

    Use

    CRISPR

    Promoter

    TaU3

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pT7-RiboJ-LacZa-T7

    Plasmid

    #173224

    Purpose

    pFGC-T7-RJBB to clone genes by Golden Gate (BsaI). To be used in cell-free systems

    Depositor

    Jim Haseloff

    Article

    Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 9, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pZac2.1 GfaABC1D ChR2(H134R) mCherry SV40

    Plasmid

    #112496

    Purpose

    Expresses channel rhodopsin in astrocytes

    Depositor

    Baljit Khakh

    Article

    Octeau et al Cell Rep. 2019 May 21;27(8):2249-2261.e7

    Insert

    ChR2(H134R)
    

    Use

    AAV

    Tags

    mCherry

    Mutation

    H134R

    Promoter

    GfaABC1D

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. MTK0_015

    Plasmid

    #123930

    Purpose

    Encodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator and constitutive NLS::tagBFP as a type 0 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB Destination - Insulator + tagBFP
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCS2mFoxd3

    Plasmid

    #37267

    Depositor

    Patricia Labosky

    Article

    Steiner et al Development. 2006 Dec;133(24):4827-38. E

    Insert

    Foxd3 (Foxd3 Mouse)
    

    Use

    Overexpression

    Expression

    Mammalian

    Available Since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

33. pHH0103_SERPINA5_Serpin

    Plasmid

    #110034

    Purpose

    Protein expression and purification of SERPINA5_Serpin

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    SERPINA5_Serpin (SERPINA5 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. gCOTS-pyl

    Plasmid

    #92048

    Purpose

    This is a S. elongatus (PCC7942) shuttle vector used for recombination of the pylRS orthogonal translation system to genomic neutral site 2.

    Depositor

    Lital Alfonta

    Article

    Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/

    Inserts

    Pyrrolysyl tRNA(cua)
    Pyrrolysyl tRNA synthetase (methanosarcina mazei)
    

    Use

    Cyanobacteria s. elongatus genome recombination i…

    Promoter

    LeuP (native S. elongatus promoter) and PrcbL

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. p2T-CMV-HNHx-ABEmax7.10HD-BlastR

    Plasmid

    #176477

    Purpose

    A-to-G base editor with alternative targeting scope

    Depositor

    Gerald Schwank

    Article

    Villiger et al Mol Ther Nucleic Acids. 2021 Aug 26;26:5

    Insert

    HNHx-ABEmax7.10HD
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. MTK3_009

    Plasmid

    #123724

    Purpose

    Encodes nuclear localized PHYB fused to activating domain of VP16 as Type 3 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PHYB-VP16-NLS
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. p6903 PHAGE-B CMV-N-EGFP Myc

    Plasmid

    #37608

    Depositor

    Peter Howley

    Article

    Martinez-Noel et al Mol Cell Biol. 2012 May 29.

    Insert

    Myc (MYC Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

38. pSEM398 - mosTI unc-119 - NNN - operon - mScarlet - tbb-2

    Plasmid

    #182355

    Purpose

    mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3-blueCKAR(T/A)

    Plasmid

    #118473

    Purpose

    Negative-control mutant for blueCKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

    Insert

    blueCKAR(T/A)
    

    Tags

    6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

    Expression

    Mammalian

    Mutation

    Contains Thr-to-Ala mutation in substrate sequenc…

    Promoter

    CMV

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMOD_C2806

    Plasmid

    #91091

    Purpose

    Module C, Promoter: FMV 34S, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4 (+ BaeI site for GT donor cloning)
    

    Use

    CRISPR

    Promoter

    FMV 34S

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge