4,143 results

1. TRX1 flag

   Plasmid

   #21283

   Depositor

   Wang Min

   Article

   Liu et al Circ Res. 2002 Jun 28. 90(12):1259-66.

   Insert

   TRX1 (TXN Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 30, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Luc.Cre empty vector

   Plasmid

   #20905

   Depositor

   Tyler Jacks

   Type

   Empty backbone

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pK-myc-Par3b

   Plasmid

   #19388

   Depositor

   Ian Macara

   Article

   Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

   Insert

   Partitioning-defective protein 3b (PARD3 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Full length, isoform b.

   Promoter

   Available since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pWZL Blast mouse E-cadherin

   Plasmid

   #18804

   Depositor

   Bob Weinberg

   Article

   Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

   Insert

   E-cadherin (Cdh1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLVX-EF1alpha-SARS-CoV-2-orf6-2xStrep-IRES-Puro

   Plasmid

   #141387

   Purpose

   Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

   Depositor

   Nevan Krogan

   Article

   Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

   Insert

   SARS-CoV-2-orf6 (ORF6 SARS-CoV-2)
   

   Use

   Lentiviral

   Tags

   2xStrep

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   EF1alpha

   Available since

   April 9, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. N-Myc-53BP1 WT pLPC-Puro

   Plasmid

   #19836

   Depositor

   Titia de Lange

   Article

   Dimitrova et al Nature. 2008 Nov 27;456(7221):524-8. doi

   Insert

   p53 Binding Protein 1 (TP53BP1 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   Available since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAV5S-F30-2xdBroccoli

   Plasmid

   #66845

   Purpose

   Expresses 5S rRNA fused to two dimeric Broccoli aptamers in the F30 scaffold in mammalian cells

   Depositor

   Samie Jaffrey

   Article

   Filonov et al Curr Protoc Chem Biol. 2016 Mar 16;8(1):

   Insert

   Two dimeric Broccoli aptamers in the F30 scaffold
   

   Use

   Tags

   5S rRNA

   Expression

   Mammalian

   Mutation

   Promoter

   5S

   Available since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 903 pLNCX HA Akt1

   Plasmid

   #9004

   Depositor

   William Sellers

   Article

   Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 14, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Flag pLJM1 RagB 99L

   Plasmid

   #19315

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   Ras-related GTP binding B (RRAGB Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Q99L

   Promoter

   Available since

   Sept. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FU-tet-o-hc-myc

    Plasmid

    #19775

    Depositor

    Konrad Hochedlinger

    Article

    Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

    Insert

    c-myc (MYC Human)
    

    Use

    Lentiviral

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    T58A

    Promoter

    Available since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDusk

    Plasmid

    #43795

    Purpose

    Empty backbone for blue-light-regulated expression of a gene of interest. Expression is repressed under blue illumination.

    Depositor

    Andreas Moeglich

    Article

    Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLVX-EF1alpha-SARS-CoV-2-nsp4-2xStrep-IRES-Puro

    Plasmid

    #141369

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-nsp4 (ORF1ab SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pRK5-myc-RhoA-Q63L

    Plasmid

    #12964

    Depositor

    Gary Bokoch

    Insert

    RhoA constitutively active (RHOA Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Q63L Constitutively active

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pdCas9-DNMT3A-PuroR_v2

    Plasmid

    #74407

    Purpose

    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR_v2 (DNMT3A Human, S. pyogenes, Synthetic)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    CBh

    Available since

    April 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. RcCMV/Rb

    Plasmid

    #1763

    Depositor

    Bob Weinberg

    Insert

    human Rb (RB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLVX-EF1alpha-SARS-CoV-2-orf9b-2xStrep-IRES-Puro

    Plasmid

    #141392

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-orf9b
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. mOrange2-N1

    Plasmid

    #54568

    Purpose

    Localization: N1 Cloning Vector, Excitation: 549, Emission: 565

    Depositor

    Michael Davidson, Roger Tsien, Nathan Shaner

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Type

    Empty backbone

    Use

    Tags

    mOrange2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR223 SARS-CoV-2 S

    Plasmid

    #149329

    Purpose

    Gateway-compatible Entry vector, with insert of the spike (S) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

    Depositor

    Fritz Roth

    Article

    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

    Insert

    S (S SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    May 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pcDNA3 hE47

    Plasmid

    #16059

    Depositor

    Robert Benezra

    Article

    Jen et al Genes Dev. 1992 Aug . 6(8):1466-79.

    Insert

    E47 (TCF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHJ421

    Plasmid

    #20680

    Depositor

    Stephen Shaw

    Article

    Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

    Insert

    Ezrin (EZR Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge