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pBABE-neo largeTgenomic

Plasmid

#10891

Depositor

William Hahn

Insert

large T and small T antigen (SV40gp6 simian virus)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
pIRESneo-FLAG/HA EYFP

Plasmid

#10825

Depositor

Thomas Tuschl

Article

Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

Insert

EYFP

Tags

FLAG and HA

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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mini-pHelper-1.0

Plasmid

#230933

Purpose

The mini version (8.4 kb) of Ad helper plasmid to provide E2A, E4orf6, and VA RNA for AAV production

Depositor

Qizhao Wang

Article

Yang et al Mol Ther Nucleic Acids. 2025 May 14;36(2

Type

Empty backbone

Use

AAV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP

Plasmid

#188777

Purpose

dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Zim3-dCas9

Use

Lentiviral

Tags

HA-2xNLS and Zim3 KRAB-NLS fusion

Promoter

EF1a

Available Since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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SepRS(2)/pSertRNA(B4)/EF-Sep

Plasmid

#173897

Purpose

The plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli.

Depositor

Jason W Chin

Article

Rogerson et al Nat Chem Biol. 2015 Jul;11(7):496-503. d

Inserts

SepRS(2)
pSertRNA
EF-Sep

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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HDM-SARS2-Spike-delta21

Plasmid

#155130

Purpose

Encodes SARS-CoV-2 Spike with 21 aa C-terminal deletions for lentiviral pseudotyping

Depositor

Jesse Bloom

Article

Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051

Insert

SARS-CoV-2 Spike-delta21 (S Severe acute respiratory syndrome coronavirus 2)

Use

Lentiviral

Expression

Mammalian

Mutation

Codon optimized to H. sapiens using IDT codon opt…

Promoter

CMV

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP6

Plasmid

#141260

Purpose

Gateway-compatible Entry vector, with insert of NSP6 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1(+) - Hyperactive AID - T7 RNA Polymerase -UGI - T2A - tdTamato

Plasmid

#138610

Purpose

Express TRACE editor together with a tdTomato reporter in pcDNA3.1(+) backbone

Depositor

Fei Chen

Article

Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Inserts

Hyperactive AID
T7 RNA Polymerase
tdTomato

Expression

Mammalian

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-DJ-N589X

Plasmid

#130878

Purpose

Encodes the AAV-DJ capsid with the N589 residue replaced with a TAG stop codon

Depositor

Prashant Mali

Article

Katrekar et al Sci Rep. 2018 Feb 26;8(1):3589. doi: 10.

Insert

AAV-DJ cap N589X

Use

AAV

Expression

Mammalian

Mutation

N589X

Promoter

Unknown

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Hygro

Plasmid

#127763

Purpose

Used for expression of sgRNA in mammalian cells

Depositor

Ken-Ichi Takemaru

Type

Empty backbone

Expression

Mammalian

Available Since

June 24, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-TurboID-3MYC-kanMX6 (pFB1420)

Plasmid

#126049

Purpose

For C-terminal tagging with TurboID in yeast including geneticin-resistant marker

Depositor

François Bachand

Article

Larochelle et al J Cell Sci. 2019 May 7. pii: jcs.232249.

Insert

TurboID

Use

Pcr-based c-terminal tagging

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pRSET-6xTR-TUBE

Plasmid

#110313

Purpose

E. coli expression and purification of 6xTR-TUBE

Depositor

Yasushi Saeki

Article

Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

Insert

6xTR-TUBE (UBQLN1 Human)

Tags

N-terminal His6-T7 tag

Expression

Bacterial

Mutation

All Arg residues in the UBA domain are mutated to…

Promoter

T7

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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AAV.TBG.PI.Cre.rBG

Plasmid

#107787

Purpose

AAV vector expressing Cre from TBG promoter

Depositor

James M. Wilson

Has Service

AAV8

Insert

Cre

Use

AAV

Expression

Mammalian

Promoter

TBG

Available Since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-Sox2

Plasmid

#101761

Purpose

Sox2 promoter reporter

Depositor

Yuh-Shan Jou

Article

Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. d

Insert

SRY-box 2 promoter (SOX2 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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ER-GCaMP6-210

Plasmid

#86919

Purpose

Fluorescent reporter for ER calcium signaling

Depositor

Timothy Ryan

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

ER-GCaMP6-210

Use

Lentiviral

Expression

Mammalian

Mutation

K78H, T302L, R303P, A317E, D324G, D360G, D380Y, T…

Promoter

CAMKII

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-EGFP

Plasmid

#75159

Purpose

Derived from LentiCRISPR v1 but expresses EGFP instead of puromycin resistance

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3-GFP

Plasmid

#74165

Purpose

Mammalian expression of EGFP

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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Beta1-GFP in pHcgreen

Plasmid

#69804

Purpose

Expresses Beta1-GFP integrin for normal cell transfection

Depositor

Martin Humphries

Article

Parsons et al J Cell Sci. 2008 Feb 1;121(Pt 3):265-71.

Insert

Beta1A integrin (ITGB1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

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APEX2 - Tubulin in pEGFP

Plasmid

#66171

Purpose

APEX2 fused to the N-terminus of alpha tubulin

Depositor

Alice Ting

Article

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

APEX2 - alpha tubulin

Tags

Flag epitope tag (DYKDDDDK)

Expression

Mammalian

Mutation

APEX2 contains 4 mutations relative to wt soybean…

Promoter

CMV

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ICUE3

Plasmid

#61622

Purpose

FRET-based biosensor for monitoring cAMP

Depositor

Jin Zhang

Article

DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

Insert

ICUE3 (RAPGEF3 Human)

Tags

ECFP and cpVenus[L194]

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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