We narrowed to 9,187 results for: Pol;

1. pScalps_puro_mIL-2Rα

   Plasmid

   #59917

   Purpose

   Expresses mouse interleukin-2 receptor alpha chain

   Depositor

   Silvia Monticelli

   Article

   Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

   Insert

   interleukin 2 receptor alpha (Il2ra Mouse)
   

   Use

   Lentiviral

   Available Since

   Oct. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

2. AAV-shRNA_Tet3

   Plasmid

   #85740

   Purpose

   shRNA against mouse Tet3

   Depositor

   Hongjun Song

   Article

   Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

   Insert

   Tet3 (Tet3 Mouse)
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSCALPs-HA-NFAT1 (4-460)-GFP

   Plasmid

   #88879

   Purpose

   Lentiviral vector expressing the fusion protein HA-NFAT1 (4-460)-GFP

   Depositor

   Silvia Monticelli

   Article

   Leoni et al Proc Natl Acad Sci U S A. 2017 Feb 6. pi

   Insert

   HA-NFAT1 (4-460)-GFP fusion protein (Nfatc2 Mouse)
   

   Use

   Lentiviral

   Tags

   GFP fusion protein and HA tag

   Expression

   Mammalian

   Mutation

   amino acids 4-460 only; L78P & L287P *see bel…

   Promoter

   SFFV (spleen focus forming virus)

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGM8

   Plasmid

   #220166

   Purpose

   Expresses a reporter for an alpha-helical OMM protein

   Depositor

   Jonathan Weissman

   Article

   Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

   Insert

   TSPO (TSPO Human)
   

   Use

   Lentiviral

   Tags

   GFP11

   Available Since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGL3-OF (mutant TCF-4 sites)

   Plasmid

   #16559

   Depositor

   Bert Vogelstein

   Article

   Shih et al Cancer Res. 2000 Mar 15. 60(6):1671-6.

   Insert

   TCF-4 binding sites (TCF4 Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   3 copies of mutant Tcf-4 binding sites

   Available Since

   March 28, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pIA1385

   Plasmid

   #166862

   Purpose

   Codon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.

   Depositor

   Irina Artsimovitch

   Article

   Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

   Insert

   SARS-COV-2 Nsp12 (ORF1ab Synthetic)
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLVX Flag RagC S75N

   Plasmid

   #112757

   Purpose

   express Flag-RagC (S75N mutant)

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   Flag-RagC (S75N) (RRAGC Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Mutation

   S75N

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTon1(acgfp1)

   Plasmid

   #233648

   Purpose

   Expression vector of AcGFP1 under control of tetracycline responsive element (TRE). Also express Tet-on advenced transactivator (rtTA) under control of minimal CMV promoter.

   Depositor

   Takeo Kubo

   Article

   Tsujioka et al Nat Commun. 2017 Sep 8;8(1):495. doi: 10

   Insert

   target sequence of guide RNA #tyr (3253-3275) 5'-GGCTCCATGTCTTCCGTCCAAGG-3'
   

   Use

   Amphibian expression, tet-on

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. hsp70: mKate2-DN Kif5a

   Plasmid

   #105972

   Purpose

   heat shock inducible transgenesis

   Depositor

   Caren Norden

   Article

   Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

   Insert

   Kif5a (kif5aa Zebrafish)
   

   Tags

   mKate2

   Expression

   Bacterial

   Mutation

   truncation of aa416-1033

   Available Since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pZDonor-Nm-Cas9-gRNA-hU6

    Plasmid

    #61366

    Purpose

    empty backbone for cloning N. meningiditis protospacers. Encodes constant region of N. meningiditis Cas9 synthetic gRNA

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDECKO-mCherry GFP

    Plasmid

    #78535

    Purpose

    Control plasmid (paired gRNAs against GFP)

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6 (for expressing sgRNA) and U6 promoter

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET14b-dabAB2

    Plasmid

    #133013

    Purpose

    pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a c-terminal strep tag fusion and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

    Depositor

    David Savage

    Article

    Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

    Inserts

    dabB2
    dabA2
    

    Tags

    6xHis, StrepII tag, and sfGFP

    Expression

    Bacterial

    Promoter

    t7

    Available Since

    Nov. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAG32-3HA-3FLAG

    Plasmid

    #154081

    Purpose

    For C-terminal 3xHA-3xFLAG-protein tagging in S. pombe using hphMX4 as resistance marker.

    Depositor

    Cornelia Kilchert

    Article

    Kilchert et al Genome Res. 2020 Jul;30(7):1012-1026. do

    Insert

    3xHA-3xFLAG-hygR
    

    Use

    Pcr-based tagging in yeast

    Tags

    3xHA-3xFLAG

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. cyclin D1-HA-pBabe-Hygro

    Plasmid

    #22703

    Depositor

    William Kaelin

    Article

    Zhang et al Cancer Cell. 2009 Nov 6. 16(5):413-24.

    Insert

    cyclin D1 (CCND1 Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Available Since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pVETL-Cmcs-Flag-Ataxin3Q22-C14A

    Plasmid

    #22119

    Depositor

    Henry Paulson

    Article

    Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C14A

    Available Since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

16. -227 hTF (mutant AP-1)

    Plasmid

    #15444

    Depositor

    Nigel Mackman

    Article

    Oeth et al Arterioscler Thromb Vasc Biol. 1997 Feb

    Insert

    Tissue factor promoter (F3 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Both AP-1 sites mutated

    Available Since

    July 27, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pNG1256

    Plasmid

    #149710

    Purpose

    Expresses RpoA, RpoB, RpoC-9His and RpoZ from Bacillus subtilis to produce core RNAP complex

    Depositor

    Peter Lewis

    Article

    Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

    Insert

    rpoZ (rpoZ )
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CIBN-GFP-TMD

    Plasmid

    #79571

    Purpose

    expression of CIBN domain fused to GFP tagged mouse Syntaxin1 TMD domain (transmembrane domain and polybasic region)

    Depositor

    Pietro De Camilli, Olof Idevall-Hagren

    Article

    Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

    Insert

    syntaxin 1A (Stx1a Mouse)
    

    Tags

    CIBN and EGFP

    Expression

    Mammalian

    Mutation

    aa 249_288 (C-terminal region (polybasic stretch …

    Promoter

    CMV

    Available Since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pVETL-Cmcs-Flag-Ataxin3Q80-C14A

    Plasmid

    #22121

    Depositor

    Henry Paulson

    Article

    Todi et al J Biol Chem. 2007 Oct 5. 282(40):29348-5

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C14A

    Available Since

    Nov. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pLVX Flag RagC W115R

    Plasmid

    #112759

    Purpose

    express Flag-RagC (W115R mutant)

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-RagC (W115R) (RRAGC Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Mutation

    W115R

    Available Since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA5FRT/TO-UBE2QL1-Strep/HA

    Plasmid

    #124666

    Purpose

    Expresses UBE2QL1 with C-terminal Strep-HA tag in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

    Insert

    UBE2QL1 (UBE2QL1 Human)
    

    Tags

    2xStrep/HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEMS1988

    Plasmid

    #49119

    Purpose

    AAV plasmid with CAGGS promoter driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-CAGGS-iCre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pLVX Flag RagC T90N

    Plasmid

    #112758

    Purpose

    express Flag-RagC (T90N mutant)

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-RagC (T90N) (RRAGC Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Mutation

    T90N

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA5-FRT/TO-N-mEGFP-PARP6

    Plasmid

    #178005

    Purpose

    Expresses N-terminally mEGFP-tagged PARP6

    Depositor

    Karla Feijs

    Article

    Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

    Insert

    PARP6 (PARP6 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLyGo-Ec-7

    Plasmid

    #163135

    Purpose

    pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the C-IgAP surface expression anchor, nanobody, and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Nanobody, C-IgAP autotransporter

    Expression

    Bacterial

    Promoter

    P(rhaB)

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CaV1.3e[8a,11,31b,Δ32,42a] mut

    Plasmid

    #26576

    Depositor

    Diane Lipscombe

    Article

    Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

    Insert

    CACNA1D (Cacna1d Rat)
    

    Tags

    6XHis and V5

    Expression

    Mammalian

    Mutation

    Contains exons 8a, 11, 31b and 42a.Deletion of ex…

    Available Since

    Jan. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

27. pcDNA5FRT/TO-UBE2QL1-CS-Strep/HA

    Plasmid

    #129513

    Purpose

    Expresses UBE2QL1 C88S (catalytically inactive mutant) with C-terminal Strep-HA tag in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

    Insert

    UBE2QL1 C88S (UBE2QL1 Human)
    

    Tags

    2xStrep/HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28a-GGG-HIS-CBM-Xmod-DocIII

    Plasmid

    #91698

    Purpose

    CBM-Xmod-DocIII handle with N-Terminal Sortase Recognition Sequence GGG for posttranslational coupling to a C-Terminal LPETGG

    Depositor

    Hermann Gaub

    Article

    Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

    Insert

    GGG-HIS-CBM-Xmod-DocIII
    

    Tags

    HIS-Tag and Sortase-Tag

    Expression

    Bacterial

    Mutation

    Cysteine deletion in CBM C63S

    Promoter

    T7

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDule2-IBBN (G2)

    Plasmid

    #85501

    Purpose

    Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-

    Insert

    IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Expression

    Bacterial

    Mutation

    Y32G L65E F108W Q109M D158S R162K

    Promoter

    lpp (constitutive)

    Available Since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBa.TfR-Halo

    Plasmid

    #162716

    Purpose

    Mammalian expression of human TFRC

    Depositor

    Marvin Bentley

    Article

    Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

    Insert

    TfR (TFRC Human) (TFRC Human)
    

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Chicken Beta actin

    Available Since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge