We narrowed to 10,562 results for: CHL

1. pTU2-D-lacZ

   Plasmid

   #72953

   Purpose

   Level 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Level 2 - D site
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTU2-B-RFP

   Plasmid

   #72955

   Purpose

   Level 2 Destination vector - Position B - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Level 2 - B site
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTU2-C-RFP

   Plasmid

   #72956

   Purpose

   Level 2 Destination vector - Position C - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Level 2 - C site
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTU2-D-RFP

   Plasmid

   #72957

   Purpose

   Level 2 Destination vector - Position D - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Level 2 - D site
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTU2-b-RFP

   Plasmid

   #72959

   Purpose

   Level 2 Destination vector - Position B - Assembly of 3 TU's from Level 1-ABC

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Level 2 - b site
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pMpGWB434

   Plasmid

   #68699

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Glucocorticoid receptor (GR)

   Expression

   Plant

   Mutation

   Promoter

   MpHSP17.8A1 promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMpGWB433

   Plasmid

   #68698

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Citrine

   Expression

   Plant

   Mutation

   Promoter

   MpHSP17.8A1 promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMpGWB410

   Plasmid

   #68675

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   3xFLAG

   Expression

   Plant

   Mutation

   Promoter

   MpEF1alpha promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMpGWB413

   Plasmid

   #68678

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Glucocorticoid receptor (GR)

   Expression

   Plant

   Mutation

   Promoter

   MpEF1alpha promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMpGWB233

    Plasmid

    #68624

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Citrine

    Expression

    Plant

    Mutation

    Promoter

    MpHSP17.8A1 promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMpGWB210

    Plasmid

    #68601

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    3xFLAG

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTd-4-15-13-1

    Plasmid

    #63826

    Purpose

    This plasmid is designed to validate the biophysical model of translational coupling

    Depositor

    Howard Salis

    Article

    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71

    Insert

    CFP, mRFP1 and GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The intergenic distance between each gene is -4 n…

    Promoter

    J23100

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTd-4-15-13-2

    Plasmid

    #63827

    Purpose

    This plasmid is designed to validate the biophysical model of translational coupling

    Depositor

    Howard Salis

    Article

    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71

    Insert

    CFP, mRFP1 and GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The intergenic distance between each gene is -4 n…

    Promoter

    J23100

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pTd-4-15-13-3

    Plasmid

    #63828

    Purpose

    This plasmid is designed to validate the biophysical model of translational coupling

    Depositor

    Howard Salis

    Article

    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71

    Insert

    CFP, mRFP1 and GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The intergenic distance between each gene is -4 n…

    Promoter

    J23100

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pTd-4-15-13-4

    Plasmid

    #63829

    Purpose

    This plasmid is designed to validate the biophysical model of translational coupling

    Depositor

    Howard Salis

    Article

    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71

    Insert

    CFP, mRFP1 and GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The intergenic distance between each gene is -4 n…

    Promoter

    J23100

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pTd-4-15-13-5

    Plasmid

    #63830

    Purpose

    This plasmid is designed to validate the biophysical model of translational coupling

    Depositor

    Howard Salis

    Article

    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71

    Insert

    CFP, mRFP1 and GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The intergenic distance between each gene is -4 n…

    Promoter

    J23100

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pTd-4-15-13-6

    Plasmid

    #63831

    Purpose

    This plasmid is designed to validate the biophysical model of translational coupling

    Depositor

    Howard Salis

    Article

    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71

    Insert

    CFP, mRFP1 and GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The intergenic distance between each gene is -4 n…

    Promoter

    J23100

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pTd-4-15-13-7

    Plasmid

    #63832

    Purpose

    This plasmid is designed to validate the biophysical model of translational coupling

    Depositor

    Howard Salis

    Article

    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71

    Insert

    CFP, mRFP1 and GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The intergenic distance between each gene is -4 n…

    Promoter

    J23100

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pTd-4-15-13-8

    Plasmid

    #63833

    Purpose

    This plasmid is designed to validate the biophysical model of translational coupling

    Depositor

    Howard Salis

    Article

    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71

    Insert

    CFP, mRFP1 and GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The intergenic distance between each gene is -4 n…

    Promoter

    J23100

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pTd-4-15-13-9

    Plasmid

    #63834

    Purpose

    This plasmid is designed to validate the biophysical model of translational coupling

    Depositor

    Howard Salis

    Article

    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71

    Insert

    CFP, mRFP1 and GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The intergenic distance between each gene is -4 n…

    Promoter

    J23100

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

21. pTd-4-15-13-11

    Plasmid

    #63836

    Purpose

    This plasmid is designed to validate the biophysical model of translational coupling

    Depositor

    Howard Salis

    Article

    Tian et al Nucleic Acids Res. 2015 Aug 18;43(14):71

    Insert

    CFP, mRFP1 and GFPmut3b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    The intergenic distance between each gene is -4 n…

    Promoter

    J23100

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

22. AEB-T4-2

    Plasmid

    #63855

    Purpose

    This pFTV1 vector contains a thyroxine binding riboswitch, controlling the translation initaition rate of luciferase gene.

    Depositor

    Howard Salis

    Article

    Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

    Insert

    T4-2 thyroxine riboswitch
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    tac

    Available since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSTARR-seq_fly-x16

    Plasmid

    #71505

    Purpose

    flySTARR-seq screening vector with x16 core promoter

    Depositor

    Alexander Stark

    Article

    Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

    Type

    Empty backbone

    Use

    Flystarr-seq screening vector

    Tags

    sgGFP (SuperGlo, Qbiogene, Inc)

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJB051 (mEos2-ZapA)

    Plasmid

    #72650

    Purpose

    Inducible expression of mEos2-ZapA in bacteria

    Depositor

    Jie Xiao

    Article

    Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

    Insert

    mEos2-ZapA
    

    Use

    Tags

    mEos2

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGL3-RpS12-Gateway

    Plasmid

    #71508

    Purpose

    flySTARR-seq validation vector with Gateway cassette downstream of the firefly luciferase gene and the RpS12 core promoter

    Depositor

    Alexander Stark

    Article

    Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

    Type

    Empty backbone

    Use

    Flystarr-seq validation vector

    Tags

    luciferase

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSTARR-seq_fly-eEF1delta

    Plasmid

    #71501

    Purpose

    flySTARR-seq screening vector with eEF1delta core promoter

    Depositor

    Alexander Stark

    Article

    Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

    Type

    Empty backbone

    Use

    Flystarr-seq screening vector

    Tags

    sgGFP (SuperGlo, Qbiogene, Inc)

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pES004 (pTet-qacR 5)

    Plasmid

    #69033

    Purpose

    qacR 5 downstream of a Tet promoter

    Depositor

    Richard Murray

    Article

    de Los Santos et al ACS Synth Biol. 2015 Aug 19.

    Insert

    qacR (qacR Synthetic)
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    W61Y, E90Q, F102S, M116Q, Y119L, E120Q

    Promoter

    pTet

    Available since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJBL-RNAINS4-C24A

    Plasmid

    #69462

    Purpose

    RNA-IN S4 C24A variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

    Depositor

    Julius Lucks

    Article

    Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

    Insert

    RNA-IN S4 with C24A mutation
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    C24A and mutations for S4 variant (from wt)

    Promoter

    J23119 and J23119 (sigma 70)

    Available since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJBL-RNAINS4-A25C

    Plasmid

    #69461

    Purpose

    RNA-IN S4 A25C variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

    Depositor

    Julius Lucks

    Article

    Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

    Insert

    RNA-IN S4 with A25C mutation
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    A25C and mutations for S4 variant (from wt)

    Promoter

    J23119 and J23119 (sigma 70)

    Available since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJBL-RNAINS4-C24AA25C

    Plasmid

    #69463

    Purpose

    RNA-IN S4 C24AA25C variant 5' UTR sequence controlling expression of superfolder GFP (UTR sequence from Mutalik et al., Nat. Chem. Biol., 2012)

    Depositor

    Julius Lucks

    Article

    Watters et al Nucleic Acids Res. 2015 Sep 8. pii: gkv8

    Insert

    RNA-IN S4 with C24A, A25C mutations
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    C24A, A25C, and mutations for S4 variant (from wt)

    Promoter

    J23119 and J23119 (sigma 70)

    Available since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pASKt15C+SrtA5woH

    Plasmid

    #65019

    Purpose

    Expresses the P94R/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

    Insert

    sortase A
    

    Use

    Tags

    S tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59, P94R, D160N, D165A, K19…

    Promoter

    tetracycline promoter/operator

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAC-EsaR-D91G/V220A

    Plasmid

    #47648

    Purpose

    pAC-EsaR with a272g and c659t mutations in esaR

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

    Insert

    esaR-D91G/V220A
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Changed Aspartic acid 91 to Glycine, and Valine 2…

    Promoter

    Plac

    Available since

    Sept. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

33. pTTSTOP2

    Plasmid

    #41752

    Depositor

    Jeff Gelles

    Article

    Thoresen et al Biochemistry. 2008 Sep 9;47(36):9514-21.

    Insert

    kinesin heavy chain (Khc Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    residues 1-401

    Promoter

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

34. pNH066

    Plasmid

    #42162

    Depositor

    Colin Dolphin

    Article

    Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

    Insert

    N-TAP Tag-RT-::mTFP1
    

    Use

    Tags

    TAP-TAg

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pET21-MRPS11

    Plasmid

    #31343

    Depositor

    Linda Spremulli

    Article

    Emdadul Haque et al Mitochondrion. 2008 Jun . 8(3):254-61.

    Insert

    MRPS11 Mature form (MRPS11 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Mature for after removal of the predicted mitocho…

    Promoter

    Available since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

36. pEMY01AB-PRO

    Plasmid

    #127714

    Purpose

    Integrative yeast promoter characterization plasmid. Clone in with BpiI. Has ChrXV homology upstream and an mVenus fragment downstream.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pEMY01CD-TER

    Plasmid

    #127751

    Purpose

    Integrative yeast terminator characterization plasmid. Clone in with BpiI. Has mVenus fragment upstream and a KanMX fragment downstream.

    Depositor

    Christopher Voigt

    Article

    Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pJK412

    Plasmid

    #155371

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pJK430

    Plasmid

    #155377

    Purpose

    PphlF_A2NT in dCRv1, 2x cascade + [dCas9 + PA4-mVenus]

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A2NT in dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGGAselect

    Plasmid

    #195714

    Purpose

    pGGAselect is a cloning vector for Golden Gate Assembly using BsaI, BsmBI, and BbsI Type IIS restriction enzymes.

    Depositor

    Eric Cantor

    Article

    Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

41. pAC-85b

    Plasmid

    #53282

    Purpose

    Contains crtE, crtI, crtY genes of Erwinia herbicola (Pantoea agglomerans) Eho10, and will produce beta-carotene in E. coli when complemented with a gene encoding phytoene synthase

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    crtE, crtY, crtI
    

    Use

    Low copy number bacterial cloning vector

    Tags

    Expression

    Mutation

    Promoter

    endogenous promoters

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCMV(CAT)T7-SB100

    Plasmid

    #34879

    Depositor

    Zsuzsanna Izsvak

    Article

    Mates et al Nat Genet. 2009 Jun;41(6):753-61. Epub 2

    Insert

    SB100X transposase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pInducer20

    Plasmid

    #44012

    Purpose

    Tet-inducible lentiviral vector for ORF expression

    Depositor

    Stephen Elledge

    Article

    Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pSUP-(2xCouRS-tRNAopt)

    Plasmid

    #197930

    Purpose

    Machinery plasmid expressing constitutive and inducible 7HCou synthetase and cognate amber suppressing tRNA. Incorporates (7-hydroxy-4-coumarin-yl) ethylglycine

    Depositor

    Kenneth Johnson

    Article

    Dangerfield et al J Biol Chem. 2020 Dec 11;295(50):17265-1

    Inserts

    CouRS
    tRNA opt
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    glnS' and lambda(ts) and proK

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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45. pGWB454

    Plasmid

    #74836

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with mRFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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46. pLenti-EF1a-hChR2(H134R)-EYFP-WPRE

    Plasmid

    #20942

    Purpose

    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to EYFP driven by EF1a promoter for optogenetic activation

    Depositor

    Karl Deisseroth

    Article

    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H134R mutation in ChR2

    Promoter

    EF1alpha

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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47. pLenti PGK Puro DEST (w529-2)

    Plasmid

    #19068

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Puro

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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48. pINDUCER21-puro_Gateway-3xFLAG

    Plasmid

    #172981

    Purpose

    Gateway Destination vector for doxycycline-inducible expression of ORF

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. pCA24N-p50-ligase

    Plasmid

    #87742

    Purpose

    IPTG-inducible expression of p50-ligase fusion for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Use

    Tags

    6xHis and human p50/NF-kB (amino acids 40-366)

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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50. pEVOL-pylT-N346A/C348A

    Plasmid

    #127411

    Purpose

    Double mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethers

    Depositor

    Wenshe Liu

    Article

    Wang et al J Am Chem Soc. 2012 Feb 15;134(6):2950-3

    Insert

    Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N346A-C348A

    Promoter

    araBAD

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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