We narrowed to 5,953 results for: 338

1. pEGFP-N1/PD-L1_ICD

   Plasmid

   #121480

   Purpose

   expression of human PD-L1 in mammalian cells

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   CD274 (CD274 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   TM+ICD

   Promoter

   CMV

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-N1/PD-L1_ECD

   Plasmid

   #121479

   Purpose

   expression of human PD-L1 in mammalian cells

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   CD274 (CD274 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   ECD+TM

   Promoter

   CMV

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST-CMV 3xFLAG-GABARAPL1-GFP

   Plasmid

   #123112

   Purpose

   Expresses 3xFLAG-GABARAPL1-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPL1

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
   

   Use

   Tags

   3xFLAG and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3 2 kb prom. and exon 1 fragment CD274

   Plasmid

   #107007

   Purpose

   Modified Luciferase expression vector

   Depositor

   Julian Downward

   Article

   Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

   Insert

   CD274 (CD274 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221 MLK1 E179K

   Plasmid

   #60541

   Purpose

   Donor vector for human MLK1 E179K mutant

   Depositor

   John Brognard

   Article

   Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

   Insert

   MLK1 (MAP3K9 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   E179K

   Promoter

   Available since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pDONR221 MLK1 D294A

   Plasmid

   #60542

   Purpose

   Donor vector for human MLK1 D294A mutant

   Depositor

   John Brognard

   Article

   Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

   Insert

   MLK1 (MAP3K9 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   D294A

   Promoter

   Available since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pAAV-TRE3_ mTFP1- Vamp2

   Plasmid

   #201214

   Purpose

   AAV expression of TRE(TRE3G)-driven, Vamp2-conjugated mTFP1 expression for fluorescent labling of axon terminals

   Depositor

   Tetsuo Yamamori

   Article

   Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

   Insert

   mTFP1 (from addgene #54613)
   

   Use

   AAV

   Tags

   mouse Vamp2

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. dCas9-NLS-gs10-cERb447

   Plasmid

   #188257

   Purpose

   Plasmid ensures constitutive expression of the C-terminal fragment of split ERbeta (aa 447-501), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

   Depositor

   Roman Jerala

   Article

   Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

   Insert

   cERb447 (ESR1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Zhou-COVID-19-Spike

   Plasmid

   #161029

   Purpose

   Extracellular domain of SARS-CoV-2 spike protein that has been engineered so that it assembles pseudo virus more efficiently. Also contains portions of VSV-G for production of psuedovirus.

   Depositor

   Dapeng Zhou

   Article

   Tong et al Vaccines (Basel). 2021 Mar 22;9(3). pii:

   Insert

   Engineered COVID-19 Spike protein, VSV-G (S SAR-CoV-2, Vesicular stomatitis virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Codon optimized

   Promoter

   Available since

   Jan. 12, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pcDNA5/FRT/TO HSPH2

    Plasmid

    #19454

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    heat shock 70kDa protein 4 (HSPA4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mutations A381T, T732A, and an insertion of "…

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-HA-PD-L1 3SA

    Plasmid

    #121493

    Purpose

    Expression of human PD-L1 3SA in mammalian cells

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    PD-L1 (CD274 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S176A, T180A, S184A

    Promoter

    CMV

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA Flag PGC1 T177A S538A

    Plasmid

    #18093

    Depositor

    Bruce Spiegelman

    Article

    Jager et al Proc Natl Acad Sci U S A. 2007 Jul 17. 1

    Insert

    PGC-1a (Ppargc1a Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T177A S538A

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

13. LentiV_Neo_SIK3_CR_T221E

    Plasmid

    #108108

    Purpose

    Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TE mutation at LKB1 phosphorylation site) with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Insert

    SIK3 (SIK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    change guanine 501 to cytosine (silent mutation),…

    Promoter

    EFS promoter

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. ACVR1-TAAS-R206H

    Plasmid

    #206184

    Purpose

    in vitro transcription of RNA encoding human ACVR1 with mutations S190A, S192A, and R206H

    Depositor

    Mary Mullins

    Article

    Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

    Insert

    activin A receptor type 1 (ACVR1 Human)
    

    Use

    In vitro transcription

    Tags

    FLAG tag

    Expression

    Mutation

    S190A, S192A, R206H

    Promoter

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTXB1-EXO1b D173A

    Plasmid

    #68269

    Purpose

    Bacterial expression of human EXO1 D173A (catalytically-dead) mutant

    Depositor

    Stefano Ferrari

    Article

    Eid et al EMBO Rep. 2010 Dec;11(12):962-8. doi: 10

    Insert

    EXO1 (EXO1 Human)
    

    Use

    Tags

    Mxe intein/chitin binding domain

    Expression

    Bacterial

    Mutation

    catalytically-dead D173A. Please see depositor co…

    Promoter

    T7

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pEGPN-ACE2

    Plasmid

    #187483

    Purpose

    Expression vector - ACE2

    Depositor

    Lewis Cantley

    Article

    Kastenhuber et al Elife. 2022 Mar 23;11. pii: 77444. doi:

    Insert

    ACE2 (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Lrrc4c-Fc-His

    Plasmid

    #72085

    Purpose

    Expresses the extracellular region of the LRRC4C protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Lrrc4c (Lrrc4c Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV7.1_3xFLAG-STK3

    Plasmid

    #172990

    Purpose

    constitutive expression of FLAG-tagged STK3

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-STK3 (STK3 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Flrt2-Fc-His

    Plasmid

    #72074

    Purpose

    Expresses the extracellular region of the FLRT2 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Flrt2 (Flrt2 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAd-Track HA PGC-1 alpha

    Plasmid

    #14427

    Depositor

    Pere Puigserver

    Article

    Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

    Insert

    PGC-1 alpha (Ppargc1a Mouse)
    

    Use

    Adenoviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Last four amino acids of PGC-1a removed to make t…

    Promoter

    Available since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only