We narrowed to 4,433 results for: chm

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MSCV-Puro-FH-AGO2-S828E-S831E

Plasmid

#92001

Purpose

Expresses FLAG-HA-AGO2 (S828E-S831E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S828E, S831E

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S824E-S828E-S831E

Plasmid

#92002

Purpose

Expresses FLAG-HA-AGO2 (S824E-S828E-S831E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824E, S828E, S831E

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S824A-T830A

Plasmid

#92003

Purpose

Expresses FLAG-HA-AGO2 (S824A-T830A)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824A, T830A

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pEHISSSO6206

Plasmid

#97065

Purpose

Expressing Sulfolobus solfataricus orf SSO6206 protein

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus orf SSO6206

Tags

N-terminal TEV protease cleavable 6xHis tag

Mutation

none

Promoter

T7

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pQCXIX_sh_hBcl10_#16

Plasmid

#75253

Purpose

retroviral shRNA vector against human BCL10, expresses GFP for transduction control

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

shRNA against hBcl10 (BCL10 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

mU6

Available Since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

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pQCXIX_sh_hBcl10_#2

Plasmid

#75254

Purpose

retroviral shRNA vector against human BCL10, expresses GFP for transduction control

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

shRNA against hBcl10 (BCL10 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

mU6

Available Since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

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pQCXIX_sh_hBcl10_#1

Plasmid

#75248

Purpose

retroviral shRNA vector against human BCL10, expresses GFP for transduction control

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

shRNA against hBcl10 (BCL10 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

mU6

Available Since

June 28, 2016

Availability

Academic Institutions and Nonprofits only

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attB-GFP-Dnmt3b6-Poly(A)-NeoR

Plasmid

#65552

Purpose

Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b6 cDNA into a MIN-tagged locus using Neomycin selection

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Dnmt3b (Dnmt3b Mouse)

Use

Mouse Targeting; Bxb1

Tags

GFP

Expression

Mammalian

Mutation

isoform 6

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-P2A-GFP

Plasmid

#222996

Purpose

Mammalian expression of SpCas9 PE7 prime editor with P2A-EGFP marker

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7-P2A-EGFP

Tags

P2A-EGFP, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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PE7_AAVS1_knockin_HDR_donor

Plasmid

#223001

Purpose

HDR donor plasmid for PE7 knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PE7-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Hdr donor

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Promoter

EF1α

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pJL1-mOrange

Plasmid

#102632

Purpose

In vitro expression of mOrange from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mOrange

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W

Plasmid

#67980

Purpose

Cas9 activity reporter with BFP and GFP.

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKBFP2AGFP cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-P2A-BSD

Plasmid

#224122

Purpose

Mammalian expression of SpCas9 PE7 prime editor with P2A-BSD marker

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7-P2A-BSD

Tags

P2A-BSD, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(gGFP)-PGKmCherry2AGFP-W

Plasmid

#67982

Purpose

Cas9 activity reporter with mCherry and GFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE

Use

CRISPR and Lentiviral

Mutation

Deleted BbsI site within WPRE

Available Since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pJL1-sfGFP

Plasmid

#102634

Purpose

In vitro expression of sfGFP from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

sfGFP

Tags

strep tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pJL1-mTagBFP2

Plasmid

#102638

Purpose

In vitro expression of mTagBFP2 from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mTagBFP2

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-PRMT3(E338Q)

Plasmid

#164696

Purpose

Expression of PRMT3(E338Q) with an N-terminal FLAG tag

Depositor

Cheryl Arrowsmith

Article

Kaniskan et al Angew Chem Int Ed Engl. 2015 Apr 20;54(1

Insert

PRMT3 (PRMT3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

E338Q

Promoter

CMV

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pDY0209 ACTB Nicking guide +48

Plasmid

#179109

Purpose

ACTB N-term Nicking guide 1 +48 guide

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

ACTB N-term Nicking guide 1 +48 guide

Use

CRISPR

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Δ18 D614G

Plasmid

#164437

Purpose

Encodes SARS-CoV-2 Spike Protein lacking 18 C-terminal amino acids and D614G mutation for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

SARS-CoV-2 Spike D614G variant truncated to remove 18 amino acids (S )

Expression

Mammalian

Available Since

Feb. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pJL1-mCherry

Plasmid

#102629

Purpose

In vitro expression of mCherry from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mCherry

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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