We narrowed to 4,879 results for: gne
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Plasmid#107926Purposehph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pROS15
Plasmid#107929Purposenat based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pROS14
Plasmid#107928PurposeLEU2 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pROS13
Plasmid#107927Purposekan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEMS1626
Plasmid#29281PurposeThe plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.DepositorInsertPle155-Intron-LacZ-NLS
UseHigh copy number homologous recombination plasmid…TagsExpressionMammalianMutationPromoterMiniPromoter Ple155 from the human PCP2 geneAvailable sinceJune 10, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMSCV-IRES-mOrange/CALR ins5
Plasmid#214769PurposeMammalian expression of human CALR ins5DepositorInserthuman CALR ins5 (CALR Human)
UseRetroviralTagsExpressionMutationPromoterMSCVAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMB-KO)
Plasmid#153441PurposeE. coli expression of Rc. XDocB binding mode B knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.DepositorInsertRc. XDocB binding mode B knock-out mutant
UseTags3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…ExpressionBacterialMutationArginine 140 and methionine 144 were mutated to a…PromoterT7 promoterAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
hsp70l: hAPOB48-mCherry.
Plasmid#140639PurposeHeat shock promoter driven expression of human APOB48-mCherry. The construct has tol2 elements, and is designed to use in zebrafish.DepositorUseZebrafish expressionTagsmCherryExpressionMutationAPOB48: amino acid 1-2180Promoterhsp70lAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
hU6-pegRNA-EFS-mScarlet Template
Plasmid#196037PurposeThe template UPEmS vector designed for cloning of a single pegRNA and subsequent co-expression with mScarlet.DepositorInsertmScarlet
UseTagsExpressionMammalianMutationPromoterhU6 and EFSAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLCA.66/2272
Plasmid#22733DepositorInsertLox66-pU(delta)TK-EM7Neo-Lox2272
UseCre/Lox; Bac recombineeringTagsExpressionMutationThis vector was designed for assembling gene targ…PromoterAvailable sinceDec. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEN313 - Rad21-Halo-Frt-PGK-EM7-NeoR-bpA-Frt targeting
Plasmid#156431PurposeTargeting vector to introduce an Halotag cassette at the mouse Rad21 locus using NEOMYCINE selection. Designed for using with sgRNA CCACGGTTCCATATTATCTGDepositorInsertRAD21, Halotag (Rad21 Mouse)
UseMouse TargetingTagsExpressionMammalianMutationnonePromoterAvailable sinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX304_C12orf49-V5
Plasmid#155110Purposelentiviral plasmid for the exogneous expression of C-terminal V5-tagged C12orf49DepositorInsertC12orf49 (SPRING1 Human)
UseLentiviralTagsV5ExpressionMutationPromoterCMV promoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-Cas9/CD4-TK2
Plasmid#104397PurposeThe designed sgRNA cloned into this plasmid directs the specific DNA cleavage exerted by Cas9 nuclease in a region of exon 5 of the human TK2 gene. The plasmid also includes the Cas9 nuclease and CD4.DepositorInsertTK2 (TK2 Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMA-KO)
Plasmid#153440PurposeE. coli expression of Rc. XDocB binding mode A knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.DepositorInsertRc. XDocB binding mode A knock-out mutant
UseTags3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…ExpressionBacterialMutationArginine 191 and leucine 195 on XDocB were mutate…PromoterT7 promoterAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX_EFS-Cre_ppt-del
Plasmid#162073PurposeCompact Cre expression vector with modified PPT designed to generate high-titer non-integrating lentivirus when packaged with PsPax2-D64V (Addgene #63586)DepositorInsertCre
UseCre/Lox and LentiviralTagsExpressionMammalianMutationcodon-optimized for expression in M. musculusPromoterEFS (part of insert)Available sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5_miniTurbo-C12orf49
Plasmid#155111Purposetransfection plasmid for the exogneous expression of N-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioIDDepositorInsertC12orf49 (SPRING1 Human)
UseTagsminiTurboExpressionMammalianMutationPromoterCMV/TO inducible promoterAvailable sinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5_C12orf49-miniTurbo
Plasmid#155112Purposetransfection plasmid for the exogneous expression of C-terminal miniTurbo-tagged C12orf49 for generation of FlpIn cell lines for BioIDDepositorInsertC12orf49 (SPRING1 Human)
UseTags3x Flag and miniTurboExpressionMammalianMutationPromoterCMV/TO inducible promoterAvailable sinceAug. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEN527 - Rad21-AID[71-114]-eGFP-FRT-Blast-FRT targeting construct
Plasmid#156452PurposeTargeting vector to introduce an AID-eGFP cassette at the mouse RAD21 (SCC1) locus using BLASTICIDIN selection. Auxin-inducible degron system. Designed to be used with sgRNA CCACGGTTCCATATTATCTGDepositorInsertRAD21, AID (Rad21 Mouse)
UseMouse TargetingTagsExpressionMammalianMutationnonePromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLY54
Plasmid#130923PurposeA CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA2B2 with sfgfp::ASV).DepositorInsertsdcas9
tetR
pspFΔHTH::λN22plus
sfgfp
UseSynthetic BiologyTagsASV tag and pspFΔHTH::λN22plusExpressionBacterialMutationMutations D10A and H840A on WT cas9. Synonymous m…PromoterAnderson promoter: J23106, PpspA-LEA2B2, and PtetAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-RnCENP-O-80-GFP
Plasmid#205182PurposeExpresses chimeric rat CENP-O with mouse CENP-O N-terminus; C-term tagged with GFP under T7 promoter - designed for in vitro transcriptionDepositorUseIn vitro transcriptionTags5 glycines linker followed by GFPExpressionMammalianMutationChimeric rat CENP-O with mouse CENP-O N terminal …PromoterT7Available sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only