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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 21,788 results for: his

Showing: 5521 - 5550 of 21788 results

  1. ITGB1

    Plasmid
    #51920
    Purpose
    Expresses full-length Integrin beta-1 precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    ITGB1 (ITGB1 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Stop Codon at amino acid 728, before CD4, bio and…
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51920-plasmid-map-sequence-id-79898

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  2. pMT-HGP-v3-NbVHH05-hIgG

    Plasmid
    #171565
    Purpose
    Expresses hIgG-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-human IgG-TEV-Avi-His) in fly cells
    Depositor
    Hidde Ploegh, Norbert Perrimon
    Article
    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:
    Insert
    NbVHH05-human IgG-TEV-Avi-His (UBE2J1 Human)
    Use
    Tags
    BiP signal peptide and hIgG1-hinge-N297A-TEV site…
    Expression
    Insect
    Mutation
    Promoter
    Available since
    June 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171565-plasmid-map-sequence-id-336181

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  3. GP1BB

    Plasmid
    #51908
    Purpose
    Expresses full-length Platelet glycoprotein Ib beta chain precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    GP1BB (GP1BB Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Stop Codon at amino acid 149, before CD4, bio and…
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51908-plasmid-map-sequence-id-79887

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  4. pRD443

    Plasmid
    #168469
    Purpose
    For the insertion pf NLS-mScarlet-I-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system
    Depositor
    Remus Dame
    Article
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS-mScarlet-I-H-NSdbd-NLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168469-plasmid-map-sequence-id-331930

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  14. pRSetB-Peredox

    Plasmid
    #73806
    Purpose
    Expresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in bacteria, with N-terminal his-tag
    Depositor
    Gary Yellen
    Article
    Mongeon et al Antioxid Redox Signal. 2016 Mar 18.
    Insert
    Peredox
    Use
    Tags
    his-tag to facilitate purification
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    73806-plasmid-map-sequence-id-136849

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  16. pREXC3GH

    Plasmid
    #47077
    Purpose
    FX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags
    Depositor
    Raimund Dutzler, Eric Geertsma
    Article
    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.
    Type
    Empty backbone
    Use
    Lactococcus lactis expression
    Tags
    3C protease site, GFP, and His
    Expression
    Mutation
    Promoter
    NIS (nisin)
    Available since
    Jan. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    47077-plasmid-map-sequence-id-67103

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  19. pEZY19

    Plasmid
    #18668
    Purpose
    pET19b converted Gateway Destination vector for expressing His tagged fusion proteins in bacteria. See note in comments section below regarding the mutated enterokinase site.
    Depositor
    Yu-Zhu Zhang
    Article
    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 26, 2008
    Availability
    Academic Institutions and Nonprofits only
    18668-plasmid-map-sequence-id-8812

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Showing: 5521 - 5550 of 21788 results