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GPI-mCherry2

Plasmid

#182869

Purpose

Monomer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Publication

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

GPI-mCherry2

Expression

Mammalian

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pOPINO_NbH11-D4

Plasmid

#155363

Purpose

Expresses nanobody H11-D4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2

Depositor

Ray Owens

Publication

Huo et al Nat Struct Mol Biol. 2020 Jul 13. pii: 1

Insert

Nb H11-D4

Tags

His6

Promoter

T7

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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CROPseq-EFS-HypaSpCas9-P2A-puro

Plasmid

#106965

Purpose

single-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screen

Depositor

Alex Hewitt

Insert

HypaSpCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EFS

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn Con/Foff EYFP

Plasmid

#55651

Purpose

Cre-on/Flp-off EYFP under the Synapsin promoter

Depositor

Karl Deisseroth

Publication

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

eYFP with introns

Use

AAV; Cre on/flp off eyfp

Expression

Mammalian

Promoter

hSyn

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Ubc-megf10-GFP

Plasmid

#40207

Depositor

Joshua Sanes

Publication

Kay et al Nature. 2012 Mar 11;483(7390):465-9. doi

Insert

Megf10 (Megf10 Mouse)

Tags

GFP fusion

Expression

Mammalian

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHR_Gal4UAS_humanTRAIL _IRES_mCherry_PGK_tBFP

Plasmid

#85432

Purpose

Lentiviral vector for Gal4 inducible human full-length TRAIL with mCherry reporter and constitutive tBFP expression

Depositor

Wendell Lim

Publication

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

human TRAIL

Use

Lentiviral

Expression

Mammalian

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pRSF-MCM3/5

Plasmid

#116950

Purpose

Expresses human MCM3 and human MCM5

Depositor

James Chong

Publication

Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

Inserts

Human MCM5 (MCM5 Human)
human MCM3 (MCM3 Human)

Tags

His6

Expression

Bacterial

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pBLADE(FP6**)-mCherry

Plasmid

#168049

Purpose

Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter. mCherry expression is controlled by the PBAD promoter.

Depositor

Barbara Di Ventura

Publication

Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

Inserts

Blue light-inducible AraC dimers in Escherichia coli
mCherry

Expression

Bacterial

Promoter

J23101** and PBAD

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2 xDvl-RFP

Plasmid

#29683

Depositor

Edward De Robertis

Publication

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

dishevelled (dvl2.L Frog)

Tags

RFP

Expression

Mammalian

Available since

June 9, 2011

Availability

Academic Institutions and Nonprofits only
pttq18

Plasmid

#69122

Purpose

regulated expression of genes in Escherichia coli

Depositor

Michael Stark

Publication

Stark et al Gene. 1987;51(2-3):255-67.

Type

Empty backbone

Expression

Bacterial

Promoter

tac

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti.CAG.H2B-cerFP-2A-mito-dsRFP.W

Plasmid

#51013

Purpose

Lentivector that expresses H2B-cerFP-2A-mito-dsRFP from internal CAG enhancer-promoter

Depositor

Rusty Lansford

Insert

H2B-cerFP-2A-mito-RFP

Use

Lentiviral

Expression

Mammalian

Promoter

CAG

Available since

June 2, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-pCMV-dSaCas9-VP64-pU6-sgRNA

Plasmid

#158990

Purpose

Vector G encodes pAAV-pCMV-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in mammalian cells

Depositor

Chung Tin

Publication

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-VP64

Use

AAV and CRISPR

Expression

Mammalian

Promoter

pCMV

Available since

Aug. 29, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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NM9-SpCas9-NLS3

Plasmid

#128177

Purpose

Expresses R. toruloides codon-optimized SpCas9 under PGK1 promoter

Depositor

Huimin Zhao

Publication

Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

Insert

SpCas9-NLS3

Use

CRISPR

Expression

Yeast

Promoter

pPGK1

Available since

April 1, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAVTRE3_tdTomato

Plasmid

#135178

Purpose

AAV expression of TRE(TRE3G)-driven, tdTomato expression for fluorescent labling

Depositor

Tetsuo Yamamori

Publication

Abe et al Front Neuroanat. 2018 Oct 30;12:89. doi:

Inserts

tdTomato
TRE3G

Use

AAV

Expression

Mammalian

Mutation

a variant of DsRed

Promoter

TRE3G

Available since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pSR651

Plasmid

#65213

Purpose

Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-1 cap gene in Sf9 insect cells

Depositor

Robert Kotin

Publication

Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

Inserts

modified AAV-2 rep78
modified AAV-1 cap

Use

AAV

Expression

Insect

Promoter

p10 and polyhedrin

Available since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKD-SepRS-EFSep

Plasmid

#34623

Depositor

Jesse Rinehart, Dieter Söll

Publication

Park et al Science. 2011 Aug 26;333(6046):1151-4.

Inserts

SepRS (MMP0688 Methanocaldococcuc maripaludis)
EF-Sep (tufB E. coli)

Expression

Bacterial

Mutation

His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…

Promoter

Ptac and Ptac (from SepRS- operon like)

Available since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LeGO-C

Plasmid

#27348

Depositor

Boris Fehse

Publication

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

U6 promoter, SFFV promoter, mCherry

Use

Lentiviral

Expression

Mammalian

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pSico-CAG-v-H-Ras-IRES-Luciferase/EGFP

Plasmid

#58959

Purpose

lentiviral expression of v-H-Ras and luciferase/EGFP reporter

Depositor

Snorri Thorgeirsson

Publication

Holczbauer et al Gastroenterology. 2013 Jul;145(1):221-31

Inserts

CMV enhancer/chicken beta actin promoter
v-H-Ras
luc2/EGFP fusion gene

Use

Lentiviral

Expression

Mammalian

Promoter

CMV enhancer/chicken beta actin

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pRS416-yZ3EV-Z3pr-yEGFP (RB3579)

Plasmid

#69100

Purpose

URA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3pr driving expression of yEGFP

Depositor

David Botstein

Inserts

yZ3EV cassette
Z3 promoter
yEGFP

Expression

Yeast

Available since

Sept. 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pME-Cas9

Plasmid

#64237

Purpose

expresses codon optimized Cas9 in zebrafish

Depositor

Wenbiao Chen

Publication

Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

Insert

codon optimized Cas9

Use

CRISPR

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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