159,530 results

1. pcDNA3.3_eGFP

   Plasmid

   #26822

   Depositor

   Derrick Rossi

   Article

   Warren et al Cell Stem Cell. 2010 Sep 29. ():.

   Insert

   5'UTR-eGFP-3'UTR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 17, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. mScarlet-EEA1

   Plasmid

   #169067

   Purpose

   Express human EEA1 in mammalian cells

   Depositor

   Gia Voeltz

   Article

   Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

   Insert

   EEA1 (EEA1 Human)
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-puro_GCaMP6s

   Plasmid

   #164589

   Purpose

   Stable integration and expression of GCaMP6s in mammalian cells

   Depositor

   Joseph Hyser

   Article

   Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

   Insert

   GCaMP6s
   

   Use

   Lentiviral

   Tags

   6xHis, T7 tag, and Xpress tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. p23-DDX21 WT

   Plasmid

   #128803

   Purpose

   overexpression of DDX21 in human cells

   Depositor

   Ling-Ling Chen

   Article

   Xing et al Cell. 2017 May 4;169(4):664-678.e16. doi

   Insert

   DDX21 WT
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pmiRGLO Dual-Luciferase-PDAP1-3'UTR

   Plasmid

   #182251

   Purpose

   Human PDAP1 3` UTR region containing 4x binding sites for miR-150 cloned downstream of luciferase reporter gene

   Depositor

   Silvia Monticelli

   Article

   Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

   Insert

   3' UTR region of PDAP1 gene
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP VAMP3

   Plasmid

   #42310

   Depositor

   Thierry Galli

   Article

   Galli et al Mol Biol Cell. 1998 Jun;9(6):1437-48.

   Insert

   VAMP 3 (VAMP3 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti6.3/EV

   Plasmid

   #120848

   Purpose

   Control vector, 20nt random sequence between gateway recombination sites

   Depositor

   Jan Rehwinkel

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PB-TO-hNIL

   Plasmid

   #172113

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Article

   Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

   Inserts

   hNGN2 (NEUROG2 Human)
   hISL1 (ISL1 Human)
   hLHX3 (LHX3 Human)
   puroR-T2A-mycNLS-mTagBFP2
   

   Use

   Tags

   T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and TRE3G

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAD33.1

   Plasmid

   #36267

   Depositor

   Christian Raetz

   Article

   Chung et al Biochemistry. 2010 May 18;49(19):4126-37

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PBAD

   Available since

   Oct. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTD-NTwinStrep_Sm

    Plasmid

    #45937

    Purpose

    use to create N-terminal Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal Twin-Strep-tag and C-terminal 6x His-tag

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Type

    Empty backbone

    Use

    Tags

    6xHis and Twin-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Trc (lactose/IPTG inducible)

    Available since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHEN2-SG4 R105A

    Plasmid

    #196072

    Purpose

    Express mutant (R105A) SG4 in E.coli

    Depositor

    Shankar Balasubramanian

    Article

    Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

    Insert

    SG4 R105A
    

    Use

    Tags

    3XFLAG and 6XHis

    Expression

    Bacterial

    Mutation

    R105A mutation in SG4

    Promoter

    T7

    Available since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. TFORF1440

    Plasmid

    #143808

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ARID4A (ARID4A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBABEpuro-gateway

    Plasmid

    #51070

    Purpose

    gateway adapted mammalian expression vector

    Depositor

    Matthew Meyerson

    Article

    Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-Cre

    Plasmid

    #198712

    Purpose

    For lentiviral expression of Cre recombinase in mammalian cells.

    Depositor

    Tyler Jacks

    Article

    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

    Insert

    Pgk-Cre
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLVpuro-CMV-N-3xFLAG

    Plasmid

    #123223

    Purpose

    N-terminal 3xFLAG tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-CMV-GFP

    Plasmid

    #67634

    Purpose

    AAV vector expressing GFP

    Depositor

    Connie Cepko

    Article

    Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1(-)-qmTQ2-cAMP

    Plasmid

    #225250

    Purpose

    mammalian expression of qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

    Depositor

    Yasushi Okada

    Article

    Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

    Insert

    qmTQ2-cAMP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-mCherry-Mango II x 24

    Plasmid

    #127587

    Purpose

    Expresses the mCherry-Mango II x 24 array from a TetON responsive promoter

    Depositor

    David Rueda

    Article

    Cawte et al Nat Commun. 2020 Mar 9;11(1):1283. doi:

    Insert

    mCherry-Mango II x24
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    pTRE Tet ON

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. mApple-LAMP1-phLuorin-N-8

    Plasmid

    #54918

    Purpose

    Localization: Lysosome Membrane, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    LAMP1 phLuorin
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Lysosomes-20 double labeled with phLuorin

    Promoter

    CMV

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-CAG-mRuby3-Gal8-P2A-Zeo

    Plasmid

    #150815

    Purpose

    Mammalian expression of mRuby3-Galectin8 (Gal8) fusion protein

    Depositor

    Jordan Green

    Article

    Rui et al Sci Adv. 2022 Jan 7;8(1):eabk2855. doi:

    Insert

    mRuby3-Gal8-P2A-Zeo (LGALS8 Synthetic, Human)
    

    Use

    Tags

    Gal8 is fused to the c-terminus of mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLentiCRISPR-RFP657

    Plasmid

    #75162

    Purpose

    Derived from LentiCRISPR v1 but expresses RFP657 instead of puromycin resistance

    Depositor

    Beat Bornhauser

    Article

    McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    RFP657

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AAV-hSyn-RiboL1-jGCaMP8f

    Plasmid

    #169249

    Purpose

    AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8f protein.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    RiboL1-jGCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Dec. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pdsRED-Mono-N1-FoxO1

    Plasmid

    #34678

    Depositor

    Domenico Accili

    Insert

    FoxO1-RFP (Foxo1 Mouse)
    

    Use

    Tags

    Flag and RFP

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 1, 2012

    Availability

    Academic Institutions and Nonprofits only

24. pET28-AviTag-DogCatcher-MBP

    Plasmid

    #171928

    Purpose

    Expresses AviTag-DogCatcher-MBP in bacterial cytoplasm. The AviTag enables site-specific biotinylation by BirA.

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    AviTag-DogCatcher-MBP
    

    Use

    Tags

    His6 and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    DogCatcher added to N-terminus of MBP via a GSGES…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. p36-p37-p38-p40-pET-Duet-1

    Plasmid

    #175049

    Purpose

    Overexpression of human Rfc2,Rfc3,Rfc4,Rfc5 in E.coli

    Depositor

    Brian Kelch

    Article

    Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

    Insert

    Replication factor C subunits 2-5 (RFC4, RFC2, RFC3, RFC5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET His6 GST TEV LIC cloning vector (2G-T)

    Plasmid

    #29707

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    6xHis, GST, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV4-Tax WT

    Plasmid

    #23284

    Depositor

    Warner Greene

    Article

    Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

    Insert

    Tax
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLJM1-Tmem192-mRFP-3xHA

    Plasmid

    #134631

    Purpose

    bait for affinity-isolation of lysosomes

    Depositor

    Roberto Zoncu

    Article

    Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

    Insert

    TMEM192 (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    3xHA, TEV, and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pXL001-TQM IL13 CAR

    Plasmid

    #154054

    Purpose

    Lentiviral expression of TQM IL13 CAR for CAR-T therapy

    Depositor

    Xiaojun Lian

    Article

    Kim et al Bioact Mater. 2020 May 7;5(3):624-635. d

    Insert

    TQM IL13 CAR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCDNA3-STAT3-S727D

    Plasmid

    #73364

    Purpose

    Expression of STAT3-S727D

    Depositor

    Jie Chen

    Article

    Kim et al J Biol Chem. 2009 Dec 18;284(51):35425-3

    Insert

    Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)
    

    Use

    Tags

    FLAG tagged

    Expression

    Mammalian

    Mutation

    E16K S727D

    Promoter

    CMV

    Available since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge