We narrowed to 17,163 results for: Emb;
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Plasmid#173149PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj19b (kcnj19b Zebrafish)
UsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 end.Available SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-kcnj1a.1
Plasmid#173125PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj1a (kcnj1a.1 Zebrafish)
UsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 end.Available SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj11l
Plasmid#173140PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj11l (kcnj11l Zebrafish)
UsePcr cloning vectorPromoterNo promoter at 5 end; T7 promoter at 3 end.Available SinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcry:mCherry,-600unc:cct3GFP
Plasmid#172528PurposealphaA-crystallin promoter (cry) drives mCherry expression in the lens. The 600bp unc-45b muscle-specific promoter drives expression of GFP-tagged zebrafish cct3.DepositorInsertcct3-GFP
ExpressionMammalianAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX208-SAE1
Plasmid#171012PurposeEctopic expression of SAE1DepositorInsertuntagged SAE1
UseLentiviralExpressionMammalianPromoterEf1-alphaAvailable SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX208-nfBFP
Plasmid#171013PurposeEctopic expression of nonfluorescent BFPDepositorInsertuntagged nfBFP
UseLentiviralExpressionMammalianMutationBFP[H66A]PromoterEf1-alphaAvailable SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-HisTim12_CtoA
Plasmid#170281PurposeExpresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli productionDepositorInsertyeast Tim12 (TIM12 Budding Yeast)
TagsTEV-cleavable N-terminal His tag (on backbone)ExpressionBacterialMutationmutated two non-conserved Cys to AlaAvailable SinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (E12,14A)-mVenus
Plasmid#168499PurposeMammalian expression of the E12,14A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe E12,14A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
ExpressionMammalianAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (K8,9R)-mVenus
Plasmid#168498PurposeMammalian expression of the K8,9R mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe K8,9R mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
ExpressionMammalianAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN(K8,9A)-mPAGFP
Plasmid#168494PurposeMammalian expression of the K8,9A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertK8,9A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.
ExpressionMammalianAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (E12,14K)-mVenus
Plasmid#168488PurposeMammalian expression of the E12,14K mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe E12,14K mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
ExpressionMammalianAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (K8,9E)-mVenus
Plasmid#168487PurposeMammalian expression of the E12,14A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe E12,14A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
ExpressionMammalianAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (K22,24A)-mVenus
Plasmid#168484PurposeMammalian expression of the K22,24A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe K22,24A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
ExpressionMammalianAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (K8,9A)-mVenus
Plasmid#168483PurposeMammalian expression of the K8,9A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe K8,9A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
ExpressionMammalianAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (G2A)-mVenus
Plasmid#168482PurposeMammalian expression of the G2A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe G2A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
ExpressionMammalianAvailable SinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
C513-E06: attB5r-pA-polyhedrinp>-attB1r
Plasmid#162948PurposeGateway attB5r/attB1r entry clone containing an AcMNPV polyhedrin promoter with upstream polyA, for use in construction of polycistronic baculovirus expression vectorsDepositorInsertAcMNPV polyhedrin promoter with upstream polyA sequence
UseSynthetic BiologyAvailable SinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E54: attB5r-RBS-His6-Halotag-nostop-attB1r
Plasmid#162965PurposeGateway attB5r/attB1r entry clone for generation of N-terminal His6-Halotag-tagged fusion proteins in bacterial expressionDepositorInsertHis6-Halotag fusion tag with upstream E. coli ribosome binding site
UseSynthetic BiologyAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E51: attB5r-RBS-His6-MBP-nostop-attB1r
Plasmid#162964PurposeGateway attB5r/attB1r entry clone for generation of N-terminal His6-MBP-tagged fusion proteins in bacterial expressionDepositorInsertHis6-Maltose-binding protein fusion tag with upstream E. coli ribosome binding site
UseSynthetic BiologyAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E15: attB5r-RU5u-His6-MBP-nostop-attB1r
Plasmid#162957PurposeGateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteinsDepositorInsertHis6-Maltose-binding protein fusion tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E13: attB5r-RU5u-His6-GST-nostop-attB1r
Plasmid#162955PurposeGateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteinsDepositorInsertHis6-Glutathione-S-transferase fusion tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E12: attB5r-RU5u-His6-eGFP-nostop-attB1r
Plasmid#162954PurposeGateway attB5r/attB1r entry clone for generation of N-terminal eGFP-tagged fusion proteinsDepositorInsertHis6-Green fluorescent protein fusion tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
C417-E03: attB4-polyhedrinp>His6-GST-nostop-attB1r
Plasmid#162934PurposeGateway attB4/attB1r entry clone for baculovirus expression of His6-GST tagged proteins using the polyhedrin promoterDepositorInsertHis6-glutathione-S-transferase fusion, AcMNPV polyhedrin promoter
UseSynthetic BiologyAvailable SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
C417-E02: attB4-polyhedrinp>His6-MBP-nostop-attB1r
Plasmid#162933PurposeGateway attB4/attB1r entry clone for baculovirus expression of His6-MBP tagged proteins using the polyhedrin promoterDepositorInsertHis6-maltose binding protein fusion, AcMNPV polyhedrin promoter
UseSynthetic BiologyAvailable SinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 VAC8-G2A,C4A,C5A,C7A-RFP
Plasmid#166842PurposeExpresses a myristoylation and palmitoylation mutant Vac8-RFP in yeasts, mutations are Gly2Ala, Cys4Ala, Cys5Ala, Cys7AlaDepositorInsertVAC8 (VAC8 Budding Yeast)
TagsRFPExpressionYeastMutationG2A, C4A, C5A, C7APromoterVAC8 promoterAvailable SinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 VAC8-G2A,C4A,C5A,C7A-GFP
Plasmid#166839PurposeExpresses a myristoylation and palmitoylation mutant Vac8-GFP in yeasts, mutations are Gly2Ala, Cys4Ala, Cys5Ala, Cys7AlaDepositorInsertVAC8 (VAC8 Budding Yeast)
TagsGFPExpressionYeastMutationG2A, C4A, C5A, C7APromoterVAC8 promoterAvailable SinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405 VAC8-C4A,C5A,C7A-GFP
Plasmid#166838PurposeExpresses a palmitoylation mutant Vac8-GFP in yeasts, mutations are Cys4Ala, Cys5Ala, Cys7AlaDepositorInsertVAC8 (VAC8 Budding Yeast)
TagsGFPExpressionYeastMutationC4A, C5A, C7APromoterVAC8 promoterAvailable SinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-31aa-Venus-pEGFP-C3
Plasmid#166754PurposeFor expression of mCerulean3 linked to Venus by 31 amino acid linkerDepositorInsertmCerulean3-31aa-Venus
ExpressionMammalianPromoterCMVAvailable SinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-TOPO-kcnt2a
Plasmid#164963PurposeZebrafish kcnt2a gene CDSDepositorInsertkcnt2a (kcnt2 Zebrafish)
UsePcr cloning vectorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-optimizedNedd4.2F823D
Plasmid#165105Purposebacterial expression of a GST fusion of Human Nedd4.2d F823DDepositorInsertNedd4.2d F823D (NEDD4L Human)
TagsGST from plasmid.ExpressionBacterialMutationcodon optimized for E. coli expression; F823D; …Available SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mEGFP-HO-G
Plasmid#164163PurposeExpresses mEGFP (monomeric EGFP with the A206K mutation) under the control of CAG promoter, codon-optimized for Homo sapiens, basic vector, multiclonig site at C terminalDepositorInsertmEGFP A236K
ExpressionMammalianPromoterCAGAvailable SinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET15-His-∆NGlpG(Y205F)
Plasmid#155132PurposeExpresses N-terminally His-tagged E. coli GlpG Y205F variant (residues 87-276) from pET15bDepositorInsertGlpG (glpG Escherichia coli)
TagsHisExpressionBacterialMutationY205F, insert codes for GlpG residues 87-276PromoterT7Available SinceSept. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
TB:3xDBE_RE:MiniP
Plasmid#124529Purpose3 Copies of the Daf-16 family binding elementDepositorInsert3 copies of the Daf-16 family binding element
UseLuciferase and Synthetic BiologyExpressionMammalianAvailable SinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
D89_Ld_343450
Plasmid#135902PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInserttransmembrane/endomembrane-like protein
Tagsbiotinylation sequence and His tagExpressionMammalianAvailable SinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
phNFL-L268P
Plasmid#132593PurposeExpresses human NFL containing the Charcot-Marie-Tooth disease type 2E L268P mutationDepositorAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
prNFL-P22S-EGFP
Plasmid#132594PurposeExpresses hEGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E P22S mutationDepositorAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
phNFL-P8R
Plasmid#132590PurposeExpresses human NFL containing the Charcot-Marie-Tooth disease type 2E P8R mutationDepositorAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
phNFL-P22S
Plasmid#132591PurposeExpresses human NFL containing the Charcot-Marie-Tooth disease type 2E P22S mutationDepositorAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCool-human TSC1 (939-992)
Plasmid#132618Purposeexpresses human TSC1 (939-992) in E. coliDepositorInsertTSC1 (939-992)
ExpressionBacterialAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MBP1-K295TAG-W340A-S233H-T237C-CAAX.pcDNA3-k
Plasmid#127403PurposeExpresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation siteDepositorInsertMaltose Binding Protein
TagsCAAX and FLAGExpressionMammalianMutationAmino acid mutations: K295 to Amber codon, W340A,…PromoterCMVAvailable SinceJuly 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MBP1-E322TAG-K305H-E309H-W340A-CAAX.pcDNA3-k
Plasmid#127407PurposeExpresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation siteDepositorInsertMaltose Binding Protein
TagsCAAX and FLAGExpressionMammalianMutationAmino acid mutations: E322 to Amber codon, K305H,…PromoterCMVAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MBP1-K295TAG-W340A-S233H-T237H-CAAX.pcDNA3-k
Plasmid#127401PurposeExpresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation siteDepositorInsertMaltose Binding Protein
TagsCAAX and FLAGExpressionMammalianMutationAmino acid mutations: K295 to Amber codon, W340A,…PromoterCMVAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pProEx-Htb-cytATL2-6Gly-sfGFP
Plasmid#124065PurposeE. coli. exp. and purif. of X. laevis cytATL fragment fused to GFP in c-terminus.DepositorInsertcytATL-sfGFP
TagssfGFPExpressionBacterialPromotertrcAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pESC/mCitrine/erg3-mCherry
Plasmid#117993PurposeCo-expression of mCitrine under the control of the Gal1 promoter and erg3 fused to mCherry (an ER-targeting marker protein) under the control of the Gal10 promoterDepositorInserterg3-mCherry
UsePlant/bacteria binary vectorExpressionPlantPromoterGal10Available SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAL928
Plasmid#119853Purposeprecusor alkaline phosphatase, phoA, with C169S, C179S, C287S, C337S, A369C and A427CDepositorInsertprecursor alkaline phosphatase, phoA, with mutations C169S, C179S, C287S, C337S, A369C and A427C
TagsnoneExpressionBacterialMutationCysteine 169 to Serine, Cysteine 179 to Serine, C…PromoterT7Available SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1-d80
Plasmid#114025PurposeInducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell linesDepositorAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-HEC1-d80
Plasmid#114030PurposeInducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell linesDepositorAvailable SinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-HEC1-8A/S15D
Plasmid#114031PurposeInducible expression of eGFP-tagged HEC1-8A/S15D in FLPin Trex cell linesDepositorAvailable SinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-1 gnu
Plasmid#112999PurposeFor protein expression and purification of full-length Drosophila gnuDepositorInsertfull length gnu (gnu Fly)
ExpressionBacterialAvailable SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBV163
Plasmid#108364PurposeUsed to replace C. glabrata YAP1 promoter with the stronger TDH3 promoter.DepositorInsertKnock-in Cg TDH3 promoter in Cg YAP1 gene
UseReplace c.glabrata yap1 promoter with c. glabrata…PromoterTDH3Available SinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:ATG-3xHA-S11
Plasmid#108238PurposeBinary vectors used for the inducible expression of tripartite split-sfGFP components (3xHA-S11 as control) in plantsDepositorInsertATG-3xHA-S11
UseSynthetic BiologyExpressionPlantPromoterUBQ10:sXVE:Available SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only