We narrowed to 10,319 results for: 158

1. p2T-cmv-SpCas9-TadDE-BlastR

   Plasmid

   #193850

   Purpose

   Express TadDE in mammalian cells with BlastR selection, Tol2 integration

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadDE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. tdp43-EGFP construct2

   Plasmid

   #28195

   Depositor

   Zuoshang Xu

   Article

   Yang et al PLoS One. 2010 . 5(12):e15878.

   Insert

   Tar DNA binding protein (TARDBP Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa86-414 only

   Promoter

   Available since

   Sept. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pBabe Angptl4 puro

   Plasmid

   #19156

   Depositor

   Joan Massague

   Article

   Padua et al Cell. 2008 Apr 4. 133(1):66-77.

   Insert

   angiopoietin-like 4 (ANGPTL4 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 3, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pQE80L-SH3

   Plasmid

   #160331

   Purpose

   This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its thermostability and quantum yield is improved compared to wild type.

   Depositor

   Sang Taek Jung

   Article

   Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12

   Insert

   SH3
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACT2-CAS9c

   Plasmid

   #51055

   Purpose

   Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.

   Depositor

   Yunde Zhao

   Article

   Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

   Insert

   Cas9c
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   yeast ADH1 promoter

   Available since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5/FRT/TO V5 DNAJB6a

   Plasmid

   #19529

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DNAJB6a (DNAJB6 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. tdp43-EGFP construct5

   Plasmid

   #28198

   Depositor

   Zuoshang Xu

   Article

   Yang et al PLoS One. 2010 . 5(12):e15878.

   Insert

   Tar DNA binding protein (TARDBP Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   aa257-414 only

   Promoter

   Available since

   Sept. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

8. Flag Intersectin Long (human)

   Plasmid

   #47393

   Depositor

   Peter McPherson

   Article

   Hussain et al Nat Cell Biol. 2001 Oct;3(10):927-32.

   Insert

   intersectin long (ITSN1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pJT396 PB ITR and Cp36 attD pEF Puro-2a-tagBFP2 donor

   Plasmid

   #184952

   Purpose

   Donor for both PiggyBac and Cp36 Recombinase

   Depositor

   Michael Bassik, Ami Bhatt, Patrick Hsu, Lacra Bintu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   PuroR, P2A, mTagBFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-CaMKII-GRAB_eCB2.0

    Plasmid

    #164608

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in CaMKII positive neurons

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    S383T

    Promoter

    CaMKII

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Man2a1-myc

    Plasmid

    #163648

    Purpose

    Expresses myc tagged Man2a1 in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    mannosidase alpha class 2A member 1 (MAN2A1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Silent mutation was made to introduce a XbaI rest…

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SpCas9 TadCBEc

    Plasmid

    #193834

    Purpose

    Express TadCBEc (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDc-SpCas9 D10A-2xUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Cas12f-GE ver4.0

    Plasmid

    #176543

    Purpose

    Expresses Cas12f-GE in mammalian cells

    Depositor

    Yong-Sam Kim

    Article

    Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

    Inserts

    Cas12f-GE ver4.0
    Cas12f-GE ver4.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and chicken β-actin

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-GNPTAB

    Plasmid

    #78107

    Purpose

    Mammalian expression of human GNPTAB, the alpha/beta subunit precursor miniconstruct that allows its efficient expression, spans the membrane twice, and lacks amino acids 431 to 819.

    Depositor

    Thomas Braulke

    Article

    Marschner et al Science. 2011 Jul 1;333(6038):87-90. doi

    Insert

    GNPTAB (GNPTAB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

15. YE1-BE4max-CP1028

    Plasmid

    #138160

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

    Insert

    rAPOBEC1(YE1)-nSpCas9 (CP1028) -UGI-UGI
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Within rAPOBEC1 W90Y + R126E; within Cas9 (CP1028…

    Promoter

    CMV

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. (318) pcDNA3.1-myc-OGA(1-400)D174N

    Plasmid

    #168096

    Purpose

    Myc tagged inactive truncated human OGA catalytic domain of residues 1-400 with D174N mutation

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    myc-OGA(1-400, D174N)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    D174N catalytically inactive OGA

    Promoter

    T7/CMV

    Available since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Cas12f-GE ver3.0

    Plasmid

    #176508

    Purpose

    Expresses Cas12f-GE in mammalian cells

    Depositor

    Yong-Sam Kim

    Article

    Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

    Inserts

    Cas12f-GE ver3.0
    Cas12f-GE ver3.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and chicken β-actin

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV_eA3A-nCas9

    Plasmid

    #163535

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    eA3A-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Promoter

    Available since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEGFP-C1-M3(T3D)471-721

    Plasmid

    #56657

    Purpose

    GFP reporter for cytoplasmic inclusions formed by the fused, C-terminal region of protein muNS from mammalian orthoreovirus Type 3 Dearing

    Depositor

    Max Nibert

    Article

    Broering et al J Virol. 2005 May;79(10):6194-206.

    Insert

    M3(T3D)421-721
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    insert encodes aa 471-721 + native stop codon of …

    Promoter

    CMV-IE

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3 MDM2 1-400

    Plasmid

    #45754

    Depositor

    Robert Lefkowitz

    Article

    Shenoy et al Science. 2001 Nov 9;294(5545):1307-13. E

    Insert

    MDM2 1-400 (MDM2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa 1-400, lacks catalytic RING domain

    Promoter

    CMV

    Available since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only