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pT5T_hp53_ST

Plasmid

#34922

Depositor

Thanos Halazonetis

Article

Petty et al EMBO J. 2011 Jun 1;30(11):2167-76. Epub

Insert

p53 DNA-binding (Res 1-393) (TP53 Human)

Expression

Bacterial

Mutation

C135V, C141V, W146Y, C182S, V203A, R209P, C229Y, …

Promoter

T7

Available Since

Feb. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pBabe EGFR ASVinsertion

Plasmid

#32066

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

Insert duplicate A767-V769

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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mEmerald-Actin-C-18

Plasmid

#53978

Purpose

Localization: Actin, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Burnette et al Nat Cell Biol. 2011 Apr;13(4):371-81. do

Insert

Actin (ACTB Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mTagRFP-T-Actin-7

Plasmid

#58001

Purpose

Localization: Actin, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Actin (ACTB Human)

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCDNA3 T7 DLC1

Plasmid

#24266

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

DLC1 (DYNLL1, DLC1 Human)

Tags

T7

Expression

Mammalian

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
ADORA3-DuET

Plasmid

#213176

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

ADORA3 (ADORA3 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pSCALPS-mAgo2-EGFP

Plasmid

#209935

Purpose

Lentiviral expression of mouse Argonaute-2 in mammalian cells

Depositor

Phillip Zamore

Article

Jouravleva et al Cell Rep Methods. 2022 Mar 18;2(3):10018

Insert

Ago2 (Ago2 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

SFFV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pHH0103_CACNB1_Guanylate-kin

Plasmid

#110036

Purpose

Protein expression and purification of CACNB1_Guanylate-kin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

CACNB1_Guanylate-kin (CACNB1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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ATG9A-2STrep

Plasmid

#210359

Purpose

Expresses ATG9A fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

ATG9A (ATG9A Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pBMN HA-GBRP

Plasmid

#89296

Purpose

For expression of the mammalian Atg8 protein GABARAP tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

GABARAP (GABARAP Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only
DLG4_PDZ_3

Plasmid

#103953

Purpose

Protein expression and purification of PSD95 PDZ3 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

DLG4 (DLG4 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pHCMV_VSVgSS-diCR2

Plasmid

#207324

Purpose

Expression of secreted diCR2-10his competitor protein which can inhibit VSV-G mediated infection

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

diCR2 (LDLR Human)

Expression

Mammalian

Mutation

fragment of LDL-R dimerized

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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plenti hSyn AldoA

Plasmid

#220916

Purpose

Neuronal specific expression of AldoA

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

AldoA (ALDOA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hSyn

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pHH0103_BCL2L1_BH4+Bcl-2

Plasmid

#109993

Purpose

Protein expression and purification of BCL2L1_BH4+Bcl-2

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

BCL2L1_BH4+Bcl-2 (BCL2L1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pSP64-hERG1a T623A

Plasmid

#53055

Purpose

Expresses alpha subunit of T623A hERG1a K channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Use

Xenopus oocyte expression

Mutation

changed Threonine 623 to Alanine

Promoter

SP6

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pBabe EGFR(G719S/T790M)

Plasmid

#32076

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

G719S/T790M

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pBabe EGFR Del4

Plasmid

#32064

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

Delete L747-P753, insert S

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pAM354 pET28a-GGG-Titin[V15P]-23-K-19-C-15

Plasmid

#226350

Purpose

Expression of proteasome substrate with tail for molecular ruler

Depositor

Andreas Martin

Article

Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

Insert

titin (TTN Human)

Expression

Bacterial

Mutation

V15P

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pETM10 with rat FL Annexin A11

Plasmid

#164496

Purpose

For bacterial expression of rat His-tagged FL Annexin A11

Depositor

Anni Vedeler

Article

Lillebostad et al Biomolecules. 2020 Apr 24;10(4). pii: bi

Insert

rat FL Annexin A11 (Anxa11 Rat)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe EGFR Del5

Plasmid

#32063

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

Delete E746-S752, insert V)

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only