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ptac-KanL94P

Plasmid

#15927

Depositor

Nina Papavasiliou

Article

Besmer et al Mol Cell Biol. 2006 Jun . 26(11):4378-85

Insert

Kanamycin Resistance

Expression

Bacterial

Mutation

L94P

Available Since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only

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RCAS(B) cFgf8 (CT#807)

Plasmid

#13892

Depositor

Cliff Tabin

Article

Brent et al Cell. 2003 Apr 18. 113(2):235-48.

Insert

Fgf8 (FGF8 Chicken)

Use

Retroviral; Avian expression

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
pTF101.2

Plasmid

#134771

Purpose

A binary plasmid containing nopaline synthase promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.

Depositor

Kan Wang

Article

Testroet et al In Vitro Cell.Dev.Biol.-Plant (2017) 53:

Type

Empty backbone

Expression

Plant

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMET7-GAG-HRAS

Plasmid

#80604

Purpose

Mammalian expression vector that expresses the HIV-1 GAG protein with HRAS fused to it

Depositor

Sven Eyckerman

Article

Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

Insert

HIV-1 p55 GAG

Expression

Mammalian

Promoter

SRalpha

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pJZC572

Plasmid

#62318

Purpose

sgRNA with 1x com for yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 1x com RNA binding module

Expression

Yeast

Promoter

SNR52

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pBY2887

Plasmid

#23221

Depositor

Ralf Baumeister

Article

Schaffer et al Nucleic Acids Res. 2010 Apr;38(6):e91. d

Insert

SnAvi

Expression

Bacterial, Insect, and Mamm…

Available Since

Feb. 25, 2010

Availability

Academic Institutions and Nonprofits only

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TEV Protease-mCherry

Plasmid

#243793

Purpose

Encodes for mammalian expression of TEV protease input. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

TEV protease

Expression

Mammalian

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pTPH405c

Plasmid

#185726

Purpose

Directed evolution of Cas9 adenine base editors with altered PAM compatibility

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

gIII(N)-NpuN-NpuC-gIII(C) single PAM linker

Use

CRISPR

Expression

Bacterial

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCAT007

Plasmid

#171634

Purpose

Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and CAG promoter expressing mTagBFP2

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-TRAC
mTagBFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CAG and U6

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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LRTi

Plasmid

#214072

Purpose

Luminescent reporter system to measure EJC-enhanced NMD activity. No intron in 5'UTR

Depositor

Sujatha Jagannathan

Article

Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

Insert

Firefly luciferase

Expression

Mammalian

Mutation

Linker sequence present between firefly luciferas…

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Marathon-RT for Split-PE (pEH59)

Plasmid

#190108

Purpose

Expresses Marathon-RT (human codon optimization)

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

Marathon-RT

Tags

bpNLS

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR221-CAG.Pro

Plasmid

#79490

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) CAG promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

CAG Promoter

Use

Gateway Cloning

Promoter

CAG Promoter

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pT-58

Plasmid

#16088

Depositor

Yoshiko Kikuchi

Article

Takahashi et al J Biochem (Tokyo). 2003 Apr . 133(4):415

Insert

UBA2 (UBA2 Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
hTex1-GST

Plasmid

#11199

Depositor

Robin Reed

Article

Masuda et al Genes Dev. 2005 Jul 1. 19(13):1512-7.

Insert

Tex1 (THOC3 Human)

Tags

GST

Expression

Bacterial

Available Since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only
pLEP1-H-Hsp82

Plasmid

#1277

Depositor

Susan Lindquist

Article

Chang et al J Biol Chem 1994 Oct 7;269(40):24983-8.

Insert

his-Hsp82 (HSP82 Budding Yeast)

Tags

Histidinex6

Expression

Yeast

Available Since

July 18, 2005

Availability

Academic Institutions and Nonprofits only
pHR-ZIP(FF)

Plasmid

#27135

Depositor

Ron Vale

Article

Yudushkin et al Proc Natl Acad Sci U S A. 2010 Dec 21;10

Insert

CD3zeta, eGFP, mCherry and ZAP70 (residues 1-259) (ZAP70, Cd247 Mouse, Human)

Use

Lentiviral

Tags

eGFP and mCherry

Expression

Mammalian

Mutation

Tyrosines in ITAMS 1-3 mutated to phenylalanines …

Available Since

April 12, 2011

Availability

Academic Institutions and Nonprofits only

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pYPQ290

Plasmid

#129670

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AacCas12b

Use

CRISPR; Gateway compatible aaccas12b entry clone

Expression

Plant

Mutation

AacCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ292

Plasmid

#129672

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b

Use

CRISPR; Gateway compatible aacas12b entry clone

Expression

Plant

Mutation

AaCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pscALPS gfp Blasti

Plasmid

#115804

Purpose

Express gfp driven by SFFV promoter

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

gfp

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pETCON-HtsptLB12

Plasmid

#45120

Depositor

David Baker

Article

Procko et al J Mol Biol. 2013 Sep 23;425(18):3563-75.

Insert

HtsptLB12

Tags

Aga2p for yeast surface display and c-myc epitope…

Expression

Yeast

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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