We narrowed to 64,611 results for: cell;

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pRSFDuet1-sgRNA(siteA)

Plasmid

#154831

Purpose

sgRNA for CRISPR/Cas9-mediated targeted integration into genomic site A in CHO cells

Depositor

Lise Marie Grav

Article

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Insert

sgRNA(siteA)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pSNAP-macroH2A1.2 Zeo

Plasmid

#132428

Purpose

Expressing SNAP-tagged histone macroH2A1.2 in mammalian cells

Depositor

John M. Greally

Article

Sato et al J Cell Biol. 2019 Jun 3;218(6):1810-1823

Insert

macroH2A1.2

Expression

Mammalian

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-survivin

Plasmid

#196908

Purpose

visualization of midzone on anaphase and midbody later during mitosis in living mammalian cells

Depositor

Kenji Sugimoto

Article

Fukada et al Biotechniques. 2004 Oct;37(4):552, 554,

Insert

survivin

Tags

EGFP

Expression

Mammalian

Mutation

E129K

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAG244 iFAST

Plasmid

#130809

Purpose

Expresses iFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Biochemistry. 2018 Oct 2;57(39):5648-565

Insert

iFAST

Tags

His-tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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Hook1-EGFP

Plasmid

#171941

Purpose

Expresses Hook1 in mammalian cells

Depositor

Cheng-han Yu

Article

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

Hook1

Tags

EGFP

Expression

Mammalian

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Rab9a

Plasmid

#169071

Purpose

Express human Rab9a in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rab9a (RAB9A Human)

Tags

acGFP1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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zCREST3:redCas9; control_gRNA

Plasmid

#199334

Purpose

pDEL157; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous control sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

zCREST3
Cas9
control sgRNA

Use

Tol2 destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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pci-EGFP-UPF1-DEAA

Plasmid

#135996

Purpose

DEAA UPF1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF1

Tags

EGFP

Expression

Mammalian

Mutation

DE647AA

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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SOX9-T2A-NLS-tdTomato-PGK-Puro

Plasmid

#89993

Purpose

Donor template for generation of SOX9-ntdTomato reporter cell lines

Depositor

Timo Otonkoski

Insert

SOX9-T2A-NLS-TdTomato-PGK-Puro (SOX9 Human)

Use

CRISPR

Promoter

none

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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mScarlet-Rab9aS21N

Plasmid

#169073

Purpose

Express human dominant negative form of Rab9a(S21N) in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rab9a (RAB9A Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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p-miniTurbo-HA

Plasmid

#209637

Purpose

Expression miniTurbo-HA in mammalian cells

Depositor

Xiang Zhou

Article

Lu et al Nucleic Acids Res. 2024 Mar 7:gkae126. d

Insert

miniTurbo

Expression

Mammalian

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pTol2_LSEC:Cas9green; erbb2_gRNA171

Plasmid

#199338

Purpose

pDEL135; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous erbb2 sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

LSEC
Cas9
erbb2 sgRNA

Use

Tol2 destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

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poRFP-oGFP

Plasmid

#154130

Purpose

Expresses orthogonal gfp and orthogonal rfp genes in bacterial cells

Depositor

Alexander Mankin

Article

Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

Inserts

orthogonal gfp
orthogonal rfp

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-TRPT1

Plasmid

#184244

Purpose

For TRPT1 expression in mammalian cells

Depositor

Karla Feijs

Article

Weixler et al Nucleic Acids Res. 2022 Sep 9;50(16):942

Insert

TRPT1 (TRPT1 Human)

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCZGY2849

Plasmid

#74112

Purpose

Enhanced mutant version of membrane targeted PH-miniSOG (Q103L) entry clone for more efficient ablation of cells when expressed under desired promoter.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

miniSOG(Q103L)

Use

Gateway topo cloning

Tags

PH

Available Since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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pMtBS

Plasmid

#32226

Depositor

Christopher Buck

Article

Schowalter et al PLoS Pathog. 2011 Jul;7(7):e1002161. Epu

Insert

MCV Small t antigen

Expression

Mammalian

Mutation

Splice site was silently mutated, and a restricti…

Available Since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only

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YIplac211-sGFP-Vrg4

Plasmid

#160904

Purpose

Expresses GFP-Vrg4 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

Insert

VRG4

Tags

GFP

Expression

Yeast

Available Since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-ShadowY

Plasmid

#193557

Purpose

Expresses mTurquoise2-ShadowY in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

mTurquoise2-ShadowY

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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poLuc1

Plasmid

#154128

Purpose

Expresses orthogonal firefly luciferase gene in bacterial cells

Depositor

Alexander Mankin

Article

Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

Insert

orthogonal firefly luciferase

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 27, 2021

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1-sgRNA(siteT9)

Plasmid

#154832

Purpose

sgRNA for CRISPR/Cas9-mediated targeted integration into genomic site T9 in CHO cells

Depositor

Lise Marie Grav

Article

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Insert

sgRNA(siteT9)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pcNB2

Plasmid

#89242

Purpose

Resurfaced Cell-penetrating 5F7 nanobody

Depositor

Brian McNaughton

Article

Bruce et al Protein Sci. 2016 Jun;25(6):1129-37. doi

Insert

pcNB2 (5F7)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pTol2_claudink:Cas9green; erbb2_gRNA171

Plasmid

#199339

Purpose

pDEL142; transgenic construct to express cell-specific Cas9 in oligodendrocytes; ubiquitous erbb2 sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

claudink
Cas9
erbb2 sgRNA

Use

Tol2 destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
zCREST3:Cas9green; EGF_gRNA

Plasmid

#199333

Purpose

pDEL158; transgenic construct to express cell-specific Cas9 in sensory neurons; ubiquitous nrg1 EGF sgRNA

Depositor

William Talbot

Article

Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

Inserts

zCREST3
Cas9
nrg1 EGF sgRNA

Use

Tol2 destination vector

Available Since

April 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Construct 33 - NFKBRp_Cas9_3xNLS_p2a-puroR

Plasmid

#81255

Purpose

Lentiviral construct for building stable cell lines expressing Cas9 in the presence of TNFa via puromycin selection

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

Cas9-3xNLS

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pET23-CBD-(-PP)-EGFP-H246,249D

Plasmid

#12598

Depositor

Klaus Hahn

Article

Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

Type

Empty backbone

Tags

6xHis and EGFP

Expression

Bacterial

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pADL*

Plasmid

#32097

Depositor

Christopher Buck

Article

Schowalter et al PLoS Pathog. 2011 Jul;7(7):e1002161. Epu

Insert

MCV Large T antigen

Expression

Mammalian

Mutation

57kT splice sites silently mutated, P156S to matc…

Available Since

Oct. 31, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
mitoZFD_ND2-Right-G1397-C

Plasmid

#180771

Purpose

Expresses mitoZFD_ND2-Right-G1397-C(DddAtox half) in mammalian cells

Depositor

Jin-Soo Kim

Article

Lim et al Nat Commun. 2022 Jan 18;13(1):366. doi:

Insert

mitoZFD for ND2 mutation

Expression

Mammalian

Promoter

pCMV

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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mitoZFD_ND2-Left-G1397-N

Plasmid

#180770

Purpose

Expresses mitoZFD_ND2-Left-G1397-N(DddAtox half) in mammalian cells

Depositor

Jin-Soo Kim

Article

Lim et al Nat Commun. 2022 Jan 18;13(1):366. doi:

Insert

mitoZFD for ND2 mutation

Expression

Mammalian

Promoter

pCMV

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcNB3

Plasmid

#89243

Purpose

Resurfaced Cell-penetrating B-lac nanobody

Depositor

Brian McNaughton

Article

Bruce et al Protein Sci. 2016 Jun;25(6):1129-37. doi

Insert

pcNB3 (B-Lac)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

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SNX9

Plasmid

#171943

Purpose

Lentiviral plasmid to express mouse SNX9 in mammalian cells

Depositor

Cheng-han Yu

Article

Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

SNX9

Use

Lentiviral

Expression

Mammalian

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
TU6m-gRNAscaffHygroR

Plasmid

#165485

Purpose

S. pyogenes Cas9 guide RNA expression in tilapia cells

Depositor

Dietmar Kueltz

Article

Hamar et al Sci Rep. 2021 Apr 12;11(1):7854. doi: 10

Type

Empty backbone

Use

CRISPR

Promoter

Oreochromis mossambicus U6

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
His6_BcLOV4_Q355N_mCherry_BamUK

Plasmid

#119762

Purpose

Encodes mCherry-tagged BcLOV4 permanently-"lit" mutant with Q355N mutation for expression in bacterial cell

Depositor

Brian Chow

Article

Glantz et al Methods Enzymol. 2019;622:249-270. doi:

Insert

BcLOV4-Q355N_mCherry

Tags

mCherry

Expression

Bacterial

Mutation

Mutated glutamine at position 355 to asparagine

Promoter

T7

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRBS wt

Plasmid

#154126

Purpose

Expresses wt rRNAs operon in bacterial cells

Depositor

Alexander Mankin

Article

Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

Insert

wt bacterial rRNAs operon under control of pL promotor

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Construct 7 - CMVp-Cas9-3xNLS-HSVpA

Plasmid

#81247

Purpose

Expresses Cas9 fused to 3xNLS driven by CMV promoter. For mammalian cell expression

Depositor

Timothy Lu

Article

Perli et al Science. 2016 Aug 18. pii: aag0511.

Insert

Cas9-3xNLS

Use

Synthetic Biology

Expression

Mammalian

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pci-EGFP-UPF1-R615A

Plasmid

#135995

Purpose

R615A UPF1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF1

Tags

EGFP

Expression

Mammalian

Mutation

R615A

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
His6_BcLOV4_C292A_mCherry_BamUK

Plasmid

#119761

Purpose

Encodes mCherry-tagged BcLOV4 "dark" mutant with C292A mutation for expression in bacterial cells

Depositor

Brian Chow

Article

Glantz et al Methods Enzymol. 2019;622:249-270. doi:

Insert

BcLOV4-C292A_mCherry

Tags

mCherry

Expression

Bacterial

Mutation

Mutated cysteine at position 292 to alanine

Promoter

T7

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
NGN3-T2A-NLS-tdTomato-ires-puro-BGHpA-PGK-puro

Plasmid

#89994

Purpose

Donor template for generation of NGN3-ntdTomato reporter cell lines

Depositor

Timo Otonkoski

Insert

NGN3-T2A-NLS-TdTomato-ires-puro-BGHpA-PGK-puro (NEUROG3 Human)

Use

CRISPR

Promoter

none

Available Since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pME18S-F-R-Olfr644

Plasmid

#149365

Purpose

Expresses Olfr644 in mammalian cells.

Depositor

Takeshi Imai

Article

Inagaki et al Cell Rep. 2020 Jun 30;31(13):107814. doi

Insert

Olfr644 (Olfr644 Mouse)

Tags

FLAG and rhodopsin

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBLIC-neo

Plasmid

#45198

Purpose

a ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells. Neo selection.

Depositor

Priyamvada Rai

Article

Patel et al BMC Biotechnol. 2012 Jan 16;12:3. doi: 1

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only
pBLIC-puro

Plasmid

#45197

Purpose

a ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells. Puro selection.

Depositor

Priyamvada Rai

Article

Patel et al BMC Biotechnol. 2012 Jan 16;12:3. doi: 1

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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We narrowed to 64,611 results for: cell;