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pcDNA-Wnt16-V5

Plasmid

#35942

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt16 (WNT16 Human)

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt3

Plasmid

#35909

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt3 (WNT3 Human)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pDA019

Plasmid

#131452

Purpose

control expression vector

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Type

Empty backbone

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-PP7v3

Plasmid

#107230

Purpose

Expresses 24x optimized PP7 stem loops (version 3) in bacterial cells

Depositor

Jeffrey Chao

Article

Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

Insert

24xPP7v3

Tags

3x FLAG-tag and P2A site

Expression

Bacterial

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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PEP101E

Plasmid

#97387

Purpose

binary construct of cytosolic sfGFP1-10 for plant

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Expression

Plant

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pET11a-SUMO3

Plasmid

#53143

Purpose

E.coli expression of untagged mature human SUMO3 (amino acids 1-93)

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

SUMO3 (SUMO2 Human)

Expression

Bacterial

Mutation

mature form (amino acids 1-93)

Promoter

T7

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v2 sgCASP8

Plasmid

#211519

Purpose

Deletes CASP8

Depositor

Richard Possemato

Article

Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

Insert

sgCASP8

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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Ii-Str_SBP-EGFP-Ecadherin

Plasmid

#65288

Purpose

synchronize trafficking of Ecadherin from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Tags

EGFP and IL-2 Signal Sequence and Streptavidin Bi…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pDGE503

Plasmid

#153276

Purpose

Intermediate sgRNA array cloning vector position 1

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Dec. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE504

Plasmid

#153277

Purpose

Intermediate sgRNA array cloning vector position 2

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLVXPuro-mGFP-Sec23B

Plasmid

#66598

Purpose

Expresses Sec23B as a fusion with mGFP

Depositor

David Stephens

Article

Hughes et al J Cell Sci. 2010 Dec 1;123(Pt 23):4032-8

Insert

Sec23B (SEC23B Human)

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCas9CyMutL

Plasmid

#166981

Purpose

Encodes aTc-inducible spCas9 and cumate-inducible MutLE32K for transient suppression of mismatch repair

Depositor

Christopher Reisch

Article

Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

Inserts

MutL (mutL )
CymR

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Mutation

E32K

Promoter

pCymRC and pLacI

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET11a-SUMO2

Plasmid

#53142

Purpose

E.coli expression of untagged mature human SUMO2 (amino acids 1-92)

Depositor

Frauke Melchior

Article

Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

Insert

SUMO2 (SUMO3 Human)

Expression

Bacterial

Mutation

mature form (amino acids 1-92)

Promoter

T7

Available Since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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413-Met25 Kar2 TorsinA E171Q GFP

Plasmid

#32127

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Tags

GFP and Kar2

Expression

Yeast

Mutation

E171Q

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
413-Met25 Kar2 TorsinA K108A GFP

Plasmid

#32125

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Tags

GFP, HDEL, and Kar2

Expression

Yeast

Mutation

K108A

Available Since

Nov. 14, 2011

Availability

Academic Institutions and Nonprofits only
pMK782 Septin6-GFP

Plasmid

#38296

Depositor

Matthew Krummel

Article

Gilden et al J Cell Biol. 2012 Jan 9;196(1):103-14.

Insert

Septin 6 (Septin6 Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

22 amino acid linker sequence (added by PCR)

Promoter

CMV

Available Since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

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Ii-Str_SBP-mCherry-Ecadherin

Plasmid

#65289

Purpose

synchronize trafficking of Ecadherin from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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MSCB-hDot1Lmut

Plasmid

#74174

Purpose

For packaging hDot1Lmut (catalytic activity dead) to retrovirus.

Depositor

Yi Zhang

Article

Okada et al Cell. 2005 Apr 22;121(2):167-78.

Insert

hDot1L (DOT1L Human)

Use

Retroviral

Tags

HA

Mutation

hDot1L 163 GSG to RCR mutation*

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA-ER-ZapCY1

Plasmid

#36321

Depositor

Amy Palmer

Article

Qin et al Proc Natl Acad Sci U S A. 2011 May 3;108

Insert

ER-ZapCY1 high affinity Zn(II) sensor

Tags

KDEL

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2012

Availability

Academic Institutions and Nonprofits only

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E.coli RaPID plasmid

Plasmid

#107251

Purpose

Expresses the E.coli RaPID labeling protein

Depositor

Paul Khavari

Article

Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

Insert

E.coli BirA*

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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