164,577 results

1. pCMV mClover3-Galectin-3

   Plasmid

   #215376

   Purpose

   Expression of the endolysosomal damage marker Galectin-3 with an N-terminal mClover3 tag.

   Depositor

   Franz-Ulrich Hartl

   Article

   Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

   Insert

   LGALS3 (LGALS3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV mRuby3-Galectin-3

   Plasmid

   #215375

   Purpose

   Expression of the endolysosomal damage marker Galectin-3 with an N-terminal mRuby3 tag.

   Depositor

   Franz-Ulrich Hartl

   Article

   Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

   Insert

   LGALS3 (LGALS3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-pHluorin_M153R-CD63

   Plasmid

   #172117

   Purpose

   Fluorescent reporter for CD63-positive exosome secretion

   Depositor

   Alissa Weaver

   Article

   Sung et al Nat Commun. 2020 Apr 29;11(1):2092. doi:

   Insert

   CD63 (CD63 Human)
   

   Use

   Lentiviral

   Tags

   pHluorin_M153R in a small extracelluar loop of CD…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Olig001

   Plasmid

   #170716

   Purpose

   Olig001 encodes a novel AAV capsid that exhibits preferential oligodendrocyte tropism in the CNS

   Depositor

   Thomas McCown

   Article

   Powell et al Gene Ther. 2016 Nov;23(11):807-814. doi:

   Insert

   Olig001 capsid gene / rep 2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLifeAct-mScarlet3_N1

   Plasmid

   #189767

   Purpose

   Construct for labelling actin filaments in mammalian cells with lifeact-mScarlet3

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   LifeAct-mScarlet3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET29a-YS14

   Plasmid

   #66890

   Purpose

   Polycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)

   Depositor

   Jung-Hyun Min

   Article

   Shim et al Anal Biochem. 2012 Aug 15;427(2):190-2.

   Inserts

   His6-Thrombin-Xenopus laevis histones H2A
   Xenopus laevis histones H2B
   Xenopus laevis histones H3
   Xenopus laevis histones H4-Thrombin
   

   Use

   Tags

   H2A, H2B, H3, H4, His6 tag, and Thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP‐RNASEH1

   Plasmid

   #108699

   Purpose

   For expression of C-terminally eGFP-tagged human RNASEH1 (nuclear isoform) in mammalian cells

   Depositor

   Martin Reijns, Andrew Jackson

   Article

   Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

   Insert

   RNASEH1 (nuclear isoform) (RNASEH1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. p177bp-LacOx162-Hyg

   Plasmid

   #210215

   Purpose

   Integrates 162xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

   Depositor

   Calvin Tiengwe

   Article

   Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

   Insert

   162xLacO repeats derived from: PMID: 12864855.
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ166-OsPE2

   Plasmid

   #141080

   Purpose

   Gateway entry clone for CRISPR-Cas9(H840A) prime editing

   Depositor

   Yiping Qi

   Article

   Tang et al Mol Plant. 2020 Mar 25. pii: S1674-2052(

   Insert

   zCas9(H840A)-OsM-MLV-RT
   

   Use

   CRISPR; Gateway compatible zcas9(h840a)-osm-mlv-rt

   Tags

   3x FLAG, NLS and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   May 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. TRE-Lepr_FL-IRES-eGFP

    Plasmid

    #196635

    Purpose

    Doxycycline inducible expression of full length mouse Lepr with eGFP marker

    Depositor

    Elaine Fuchs

    Article

    Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

    Insert

    Lepr (Lepr Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR221 msNLRP3

    Plasmid

    #218639

    Purpose

    Gateway shuttling vector for cloning C-terminal NLRP3 tags (contains no stop codon).

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (Nlrp3 Mouse)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEntry_HCoV-OC43-Spike

    Plasmid

    #168951

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    HCoV-OC43-Spike (S )
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep

    Plasmid

    #92099

    Purpose

    Doyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Type

    Empty backbone

    Use

    CRISPR and TALEN; Zfn

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-On 3G Bidirectional

    Available Since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lck-mTurquoise2

    Plasmid

    #98822

    Purpose

    In vivo visualization of the plasma membrane (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Lck
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmScarlet-I3_C1

    Plasmid

    #189756

    Purpose

    C1 Cloning vector for mammalian cells incorporating mScarlet-I3 red fluorescent protein

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    mScarlet-I3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO5.sgRNA.EFS.tRFP657

    Plasmid

    #57824

    Purpose

    Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP657, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    sgRNA
    EFS
    tagRFP657
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSL1765 (pSPIN-R, R*MmeI)

    Plasmid

    #160734

    Purpose

    Single-plasmid V. cholerae CAST. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA with BsaI sites. Reverse (R) order minimizes self-targeting; MmeI site for Tn-seq; pBBR1 backbone.

    Depositor

    Samuel H. Sternberg

    Article

    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

    Insert

    VchCAST proteins, crRNA, and donor DNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBB540

    Plasmid

    #27393

    Depositor

    Bernd Bukau

    Article

    de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

    Insert

    grpE, clpB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLyGo-Kp-1

    Plasmid

    #163143

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-MF pre-sequence and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available Since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-MS2CP-VPg(FCV)

    Plasmid

    #167314

    Purpose

    Template pDNA to prepare CaVT mRNA

    Depositor

    Hirohide Saito

    Article

    Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

    Insert

    CaVT
    

    Use

    Synthetic Biology

    Tags

    DYK-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. Cbh_v5 AAV-CBE N-terminal

    Plasmid

    #137175

    Purpose

    AAV genome: expresses the N-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    v5 AAV-CBE N-terminal; U6-protospacer
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Cas9 D10A

    Promoter

    Cbh

    Available Since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. TIRAP-GFP

    Plasmid

    #52739

    Purpose

    Retroviral vector expresses WT hTIRAPfused to GFP. Also contains IRES-hCD2 as selectable marker

    Depositor

    Jonathan Kagan

    Article

    Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10

    Insert

    Toll/IL-1 Receptor adaptor protein (TIRAP Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

23. pLV_hUbC-dSpCas9-2xVP64-T2A-BSD

    Plasmid

    #162333

    Purpose

    Lentiviral expression of dSpCas9-2xVP64 with blasticidin resistance

    Depositor

    Charles Gersbach

    Article

    Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

    Insert

    dSpCas9-2xVP64
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A and H840A

    Promoter

    hUbC

    Available Since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pRT043-CLYBL-DDdcas9-VPH

    Plasmid

    #158091

    Purpose

    Inducible expression of dCas9-VPH from the CLYBL locus for CRISPR activation

    Depositor

    Martin Kampmann

    Article

    Tian et al Nat Neurosci. 2021 May 24. pii: 10.1038/

    Insert

    CLYBL-CAG-DDdCas9VPH-T2A-GFP
    

    Use

    TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

    Plasmid

    #83946

    Purpose

    Cas9-carrying vector with kanMX marker

    Depositor

    Irina Borodina

    Article

    Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. shCD44-2 pRRL

    Plasmid

    #19123

    Depositor

    Bob Weinberg

    Article

    Godar et al Cell. 2008 Jul 11. 134(1):62-73.

    Insert

    small hairpin RNA against CD44 (CD44 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Oct. 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. mCherry-TOMM20-N-10

    Plasmid

    #55146

    Purpose

    Localization: Outer Mitochondrial Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    TOMM20 (TOMM20 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGL4.23-Fosprom

    Plasmid

    #188113

    Purpose

    Expresses luciferase under control of Fos promoter in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Shchuka et al PLoS One. 2023 Jan 3;18(1):e0271081. doi

    Insert

    Fos promoter (Fos Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Fos endogenous promoter

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHAGE-DDX3X

    Plasmid

    #116730

    Purpose

    Lentiviral expression of DDX3X

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    DDX3X (DDX3X Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGW-GFP-TurboID-GOI

    Plasmid

    #209381

    Purpose

    Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    TurboID gene sequence C249A

    Promoter

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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