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pORANGE GFP-Nlgn3 KI

Plasmid

#131501

Purpose

Endogenous tagging of Neuroligin-3: N-terminal (amino acid position: T37)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Nlgn3, Nlgn3 Rat, Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pSEVA228-pro4IUPi

Plasmid

#122018

Purpose

Expresses IUP pathway genes (ChK, IPK, idi) under constitutive promoter. Kan. resist.

Depositor

Gregory Stephanopoulos

Article

Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

Inserts

Choline kinase (CKI1 Budding Yeast)
Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
isopentenyl-diphosphate Delta-isomerase

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pro4

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNAI-GAL4-CREB

Plasmid

#46769

Purpose

Expression of GAL4 fusion to wild type CREB

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

GAL4(1-147)-CREB (Creb1, GAL4 Budding Yeast, Rat)

Tags

GAL4

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA4c/His-Xpress-HA-hBIG1(E793K)

Plasmid

#79433

Purpose

Expresses N-terminally His6, Xpress and HA-tagged BIG1(E793K) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Shin et al Mol Biol Cell. 2004 Dec;15(12):5283-94.

Insert

BIG1 (ARFGEF1 Human)

Tags

HA, His6, and Xpress

Expression

Mammalian

Mutation

changed Glutamic acid 793 to Lysine

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only
CHRM4-Tango

Plasmid

#66251

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

CHRM4 (CHRM4 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pmBanana-Hyg-CTCF-NtermNES-N1

Plasmid

#131850

Purpose

Expresses the [N-terminus of human CTCF (a.a. 2-265)]-mBanana with a nuclear export signal in mammalian cells

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

CTCF (CTCF Human)

Tags

mBanana

Expression

Mammalian

Mutation

deleted amino acids 796-2181 (the N-terminus is l…

Available Since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-hSOX17FNV

Plasmid

#206384

Purpose

Expresses human SOX17FNV in mammalian cells, for lentivirus generation.

Depositor

Ralf Jauch

Article

Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

Insert

SOX17FNV (SOX17 Human)

Use

Lentiviral

Available Since

Oct. 6, 2023

Availability

Academic Institutions and Nonprofits only

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MC2R-Tango

Plasmid

#66428

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

MC2R (MC2R Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221_SLC38A9

Plasmid

#132070

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC38A9 (SLC38A9 Human)

Expression

Mammalian

Available Since

Nov. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-EF1a-EGFP-TA MAO-IRES-Puromycin

Plasmid

#133025

Purpose

Expresses EGFP-tagged tail anchor sequence of monoamine oxidase A

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Monoamine oxidase type A (MAOA Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-488

Promoter

Human elongation factor 1 alpha

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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GG-EBNA-MIR302-7guides-PGK-Puro

Plasmid

#201960

Purpose

EBNA episome plasmid for U6 promoter-driven expression of 7 gRNAs targeting miRNA302/367. Includes PGK-puro selection cassette

Depositor

Timo Otonkoski

Article

Sokka et al Stem Cell Reports. 2022 Feb 8;17(2):413-

Insert

MIR302-7g-PGK-Puro

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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Plxnd1-Fc-His

Plasmid

#72132

Purpose

Expresses the extracellular region of the PlexinD1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxnd1 (Plxnd1 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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CRABP2

Plasmid

#155483

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CRABP2 (CRABP2 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-CREM

Plasmid

#178442

Purpose

doxycycline-inducible expression of Human CREM in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

CREM (CREM Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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mRuby2-MAPTau-C-10

Plasmid

#55904

Purpose

Localization: Microtubules, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

MAPTau (MAPT Human)

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pLJM60-FLAG-SLC38A9.1 (delta110)

Plasmid

#71861

Purpose

lentiviral stable expression

Depositor

David Sabatini

Article

Wang et al Science. 2015 Jan 9;347(6218):188-94. do

Insert

SLC38A9.1 (SLC38A9 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized, missing first 110 amino acids

Promoter

CMV

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
Tet-myr-CSK K222R-GFP

Plasmid

#83471

Purpose

Lentiviral vector for doxycycline-inducible expression of membrane targeted kinase dead CSK in mammalian cells

Depositor

Barry Gumbiner

Article

Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

Insert

CSK (CSK Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

K222R (kinase dead)

Promoter

doxycycline-inducible promoter

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pHH0103_BCAR1-1/1

Plasmid

#91418

Purpose

Protein expression and purification of human SH3 domain construct BCAR1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

BCAR1-1/1 (BCAR1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTet-O-E2F7-T2A-PuroR

Plasmid

#162348

Purpose

Lentiviral expression of E2F7 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

E2F7 (E2F7 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Promoter

Tet-ON

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-flex-TVA

Plasmid

#69618

Purpose

Express recepter TVA for envA with AAV

Depositor

Naoshige Uchida

Article

Menegas et al Elife. 2015 Aug 31;4. doi: 10.7554/eLife

Insert

Avian sarcoma and leukemia virus receptor 950 (CD320 Synthetic, quail)

Use

AAV and Cre/Lox

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 6, 2015

Availability

Academic Institutions and Nonprofits only

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