4,177 results

1. pET21a-alpha-synuclein

   Plasmid

   #51486

   Purpose

   Inducible expression of human alpha-synuclein in E.coli

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   alpha-synuclein (SNCA Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 31, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pSpCas9(BB)-2A-Blast

   Plasmid

   #118055

   Purpose

   Used for expression of sgRNA in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pInducer20

   Plasmid

   #44012

   Purpose

   Tet-inducible lentiviral vector for ORF expression

   Depositor

   Stephen Elledge

   Article

   Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUW-M2rtTA

   Plasmid

   #20342

   Purpose

   Lentiviral plasmid expressing the reverse tetracycline transactivator

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

   Insert

   M2rtTA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. scramble shRNA

   Plasmid

   #1864

   Purpose

   3rd gen lentiviral negative control vector containing scrambled shRNA

   Depositor

   David Sabatini

   Article

   Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

   Insert

   scramble
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   April 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET29b-10xHis-Super TEV

   Plasmid

   #193833

   Purpose

   Expresses high-efficiency TEV protease in bacterial cells

   Depositor

   Florence Pojer, EPFL Protein Production and Structure Core Facility (PTPSP)

   Article

   Lau et al protocols.io, doi: 10.17504/protocols.io

   Insert

   TEV protease
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   T17S, L56V, N68D, I77V, S135G, I138T, S153N, T180…

   Available Since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-EF1a-IRES-Puro

   Plasmid

   #85132

   Purpose

   lentiviral backbone

   Depositor

   Tobias Meyer

   Article

   Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Feb. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCRISPR v2-Blast

   Plasmid

   #83480

   Purpose

   Mammalian expression of Cas9 and sgRNA scaffold

   Depositor

   Mohan Babu

   Insert

   Blasticidin S deaminase
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   Replaced puromycin N-acetyltransferase on the ori…

   Promoter

   EF-1α

   Available Since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiCas9-Blast

   Plasmid

   #52962

   Purpose

   Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Has Service

   Lentiviral Prep

   Inserts

   Cas9
   Blasticidin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EFS-NS

   Available Since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. DHB-mVenus

    Plasmid

    #136461

    Purpose

    Fluorescently tagged segment of DNA Helicase B that translocates from the nucleus to the cytoplasm in response to phosphorylation by CDK2

    Depositor

    Sabrina Spencer, Tobias Meyer

    Article

    Spencer et al Cell. 2013 Oct 10;155(2):369-83. doi: 10

    Insert

    DNA Helicase B (HELB Human)
    

    Use

    Lentiviral

    Tags

    mVenus

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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