4,124 results

1. 7TGP

   Plasmid

   #24305

   Depositor

   Roel Nusse

   Article

   Fuerer et al PLoS One. 2010 . 5(2):e9370.

   Insert

   7xTcf-eGFP // SV40-PuroR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   EGFP is GFP with S65T and F64L mutations

   Promoter

   Available since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCFD3-dU6:3gRNA

   Plasmid

   #49410

   Purpose

   expression of gRNA under control of the Drosophila U6:3 promoter

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Insert

   dU6-3:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   dU6-3

   Available since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUW mCherry-GFP-LC3

   Plasmid

   #110060

   Purpose

   To visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes that have fused with the lysosome (autolyosomes; mCherry fluorescence only, due to acid sensitivity of GFP)

   Depositor

   Anne Brunet

   Article

   Leeman et al Science. 2018 Mar 16;359(6381):1277-1283

   Insert

   mCherry-GFP-LC3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti CMV Hygro DEST (w117-1)

   Plasmid

   #17454

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Hygro

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral; Destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_AncBE4max_P2A_GFP

   Plasmid

   #112100

   Purpose

   C:G-to-T:A base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

   Insert

   AncBE4max_P2A_GFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDH-EF1a-eFFly-mCherry

   Plasmid

   #104833

   Purpose

   3rd generation lentiviral vector. eFFly luciferase for in vivo Imaging, mCherry for fluorescent detection

   Depositor

   Irmela Jeremias

   Article

   Ebinger et al Cancer Cell. 2016 Dec 12;30(6):849-862.

   Inserts

   eFFly
   mCherry
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-TRE-EGFP-EF1a-rtTA

   Plasmid

   #104454

   Purpose

   PiggyBac plasmid expressing EGFP under the control of a doxycycline-inducible promotor

   Depositor

   Volker Busskamp

   Article

   Sauter et al Methods Mol Biol. 2019;1942:101-121. doi

   Inserts

   enhanced green fluorescent protein
   reverse tetracycline-controlled transactivator 3
   

   Use

   Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCTcon2

   Plasmid

   #41843

   Depositor

   Dane Wittrup

   Article

   Chao et al Nat Protoc. 2006;1(2):755-68.

   Insert

   CD20
   

   Use

   Tags

   HA and c-myc

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-PE6b

   Plasmid

   #207852

   Purpose

   Mammalian expression of PE6b prime editor

   Depositor

   David Liu

   Article

   Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

   Insert

   PE6b
   

   Use

   Tags

   SV40 bpNLS and SV40 bpNLS, c-Myc NLS

   Expression

   Mammalian

   Mutation

   Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-SFFV-KRAB-dCas9-P2A-mCherry

    Plasmid

    #60954

    Purpose

    2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherry

    Depositor

    Jonathan Weissman

    Article

    Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

    Insert

    KRAB-dCas9-P2A-mCherry fusion
    

    Use

    CRISPR and Lentiviral

    Tags

    2x NLS, HA, KRAB domain, and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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