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Showing: 541 - 570 of 13763 results

  3. pJR89

    Plasmid
    #140096
    Purpose
    sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
    Available Since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140096-plasmid-map-sequence-id-292016

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  4. MSCV-opEBNA2-IRES-GFP

    Plasmid
    #220354
    Purpose
    Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression and carries a C-terminal 3xHA-tag)
    Depositor
    Klaus Rajewsky
    Article
    Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d
    Insert
    opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)
    Use
    Retroviral
    Tags
    3 x HA tag
    Expression
    Mammalian
    Mutation
    codon-optimised for expression in mammalian cells
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    220354-plasmid-map-sequence-id-437068

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  5. pMDC32B-AtTAS1c-D2-B/c-AtMIR173

    Plasmid
    #137885
    Purpose
    Plant expression vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor downstream 3'D1[+]. Contains AtMIR173 for syntasi expression in any plant species.
    Depositor
    Alberto Carbonell
    Article
    Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583
    Inserts
    AtTAS1c-D2-B/c
    2x35S-AtMIR173-Tnos
    Expression
    Plant
    Mutation
    A. thaliana TAS1c precursor sequence including a …
    Available Since
    May 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    137885-plasmid-map-sequence-id-275856

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  9. pcDNA3-SARS-CoV-2-S-RBD-8his-N501Y

    Plasmid
    #164845
    Purpose
    Mammalian expression plasmid for RBD of SARS-CoV-2 (N501Y mutant) with 8his tag
    Depositor
    Erik Procko
    Article
    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf
    Insert
    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 (S SARS coronavirus 2)
    Tags
    8his tag and HA leader sequence
    Expression
    Mammalian
    Mutation
    Codon-optimized for human cell expression. Has N5…
    Promoter
    CMV
    Available Since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    164845-plasmid-map-sequence-id-322928

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  12. Drd1a->M71-IRES-tauGFP ACNF TV

    Plasmid
    #105073
    Purpose
    Targeting vector: the coding sequence of M71 is replaced by the sequence encoding amino acids 1-447 of Drd1a and an IRES-tauGFP followed by ACNF cassette
    Depositor
    Peter Mombaerts
    Article
    Katidou et al Mol Cell Neurosci. 2018 Apr;88:138-147.
    Insert
    Drd1a->M71-IRES-tauGFP-ACNF (Drd1, Olfr151 Mouse)
    Use
    Mouse Targeting
    Mutation
    Drd1a coding sequence followed by IRES-tauGFP ACNF
    Available Since
    Nov. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    105073-plasmid-map-sequence-id-204136

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  14. pCMV-Myr-HA-mIFITM3-C71,72,105A-CLVL

    Plasmid
    #58414
    Purpose
    Expresses murine IFITM3-C71,72,105A with an N-terminal myristoylation sequence from HIV Gag followed by an HA tag, as well as a C-terminal CaaX prenylation motif, in mammalian cells
    Depositor
    Howard Hang, Jacob Yount
    Article
    Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41
    Insert
    IFITM3 (Ifitm3 Mouse)
    Tags
    HA internal
    Expression
    Mammalian
    Mutation
    Cysteines 71, 72, and 105 to Alanine; The sequenc…
    Promoter
    CMV IE
    Available Since
    Sept. 8, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. pKlab1157-HBEGF-2a-3xFLAG-BRCA1(full-length)-P1749S-2a-mRuby3-HBBIVS2-WPRE

    Plasmid
    #248997
    Purpose
    This plasmid expresses the BRCA1 sequence with P1749S mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
    Depositor
    Georgios Karras
    Article
    Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086
    Insert
    BRCA1 (BRCA1 Human)
    Use
    CRISPR
    Tags
    3x-FLAG-V5
    Mutation
    P1749S
    Available Since
    Jan. 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    248997-plasmid-map-sequence-id-495753

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  16. pKlab1158-HBEGF-2a-3xFLAG-BRCA1(full-length)-R1699Q-2a-mRuby3-HBBIVS2-WPRE

    Plasmid
    #248998
    Purpose
    This plasmid expresses the BRCA1 sequence with R1699Q mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
    Depositor
    Georgios Karras
    Article
    Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086
    Insert
    BRCA1 (BRCA1 Human)
    Use
    CRISPR
    Tags
    3x-FLAG-V5
    Mutation
    R1699Q
    Available Since
    Jan. 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    248998-plasmid-map-sequence-id-495728

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  17. pKlab1159-HBEGF-2a-3xFLAG-BRCA1(full-length)-R1699W-2a-mRuby3-HBBIVS2-WPRE

    Plasmid
    #248999
    Purpose
    This plasmid expresses the BRCA1 sequence with R1699W mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
    Depositor
    Georgios Karras
    Article
    Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086
    Insert
    BRCA1 (BRCA1 Human)
    Use
    CRISPR
    Tags
    3x-FLAG-V5
    Mutation
    R1699W
    Available Since
    Jan. 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    248999-plasmid-map-sequence-id-495730

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  18. pKlab1160-HBEGF-2a-3xFLAG-BRCA1(full-length)-S1655F-2a-mRuby3-HBBIVS2-WPRE

    Plasmid
    #249000
    Purpose
    This plasmid expresses the BRCA1 sequence with S1655F mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
    Depositor
    Georgios Karras
    Article
    Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086
    Insert
    BRCA1 (BRCA1 Human)
    Use
    CRISPR
    Tags
    3x-FLAG-V5
    Mutation
    S1655F
    Available Since
    Jan. 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    249000-plasmid-map-sequence-id-495994

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  19. pKlab1161-HBEGF-2a-3xFLAG-BRCA1(full-length)-V1736A-2a-mRuby3-HBBIVS2-WPRE

    Plasmid
    #249001
    Purpose
    This plasmid expresses the BRCA1 sequence with V1736A mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
    Depositor
    Georgios Karras
    Article
    Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086
    Insert
    BRCA1 (BRCA1 Human)
    Use
    CRISPR
    Tags
    3x-FLAG-V5
    Mutation
    V1736A
    Available Since
    Jan. 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    249001-plasmid-map-sequence-id-495997

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  20. pKlab1069-HBEGF-2a-3xFLAG-BRCA1(full-length)-A1708V-2a-mRuby3-HBBIVS2-WPRE

    Plasmid
    #248989
    Purpose
    This plasmid expresses the BRCA1 sequence with A1708V mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
    Depositor
    Georgios Karras
    Article
    Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086
    Insert
    BRCA1 (BRCA1 Human)
    Use
    CRISPR
    Tags
    3x-FLAG-V5
    Mutation
    A1708V
    Available Since
    Jan. 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    248989-plasmid-map-sequence-id-495734

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  21. pKlab1066-HBEGF-2a-3xFLAG-BRCA1(full-length)-L1705R-2a-mRuby3-HBBIVS2-WPRE

    Plasmid
    #248990
    Purpose
    This plasmid expresses the BRCA1 sequence with L1705R mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
    Depositor
    Georgios Karras
    Article
    Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086
    Insert
    BRCA1 (BRCA1 Human)
    Use
    CRISPR
    Tags
    3x-FLAG-V5
    Mutation
    L1705R
    Available Since
    Jan. 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    248990-plasmid-map-sequence-id-495976

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  22. pKlab1070-HBEGF-2a-3xFLAG-BRCA1(full-length)-Y1845D-2a-mRuby3-HBBIVS2-WPRE

    Plasmid
    #248991
    Purpose
    This plasmid expresses the BRCA1 sequence with Y1845D mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
    Depositor
    Georgios Karras
    Article
    Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086
    Insert
    BRCA1 (BRCA1 Human)
    Use
    CRISPR
    Tags
    3x-FLAG-V5
    Mutation
    Y1845D
    Available Since
    Jan. 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    248991-plasmid-map-sequence-id-495978

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  23. pKlab1067-HBEGF-2a-3xFLAG-BRCA1(full-length)-S1715R-2a-mRuby3-HBBIVS2-WPRE

    Plasmid
    #248992
    Purpose
    This plasmid expresses the BRCA1 sequence with S1715R mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
    Depositor
    Georgios Karras
    Article
    Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086
    Insert
    BRCA1 (BRCA1 Human)
    Use
    CRISPR
    Tags
    3x-FLAG-V5
    Mutation
    S1715R
    Available Since
    Jan. 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    248992-plasmid-map-sequence-id-496009

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  24. pKlab1153-HBEGF-2a-3xFLAG-BRCA1(full-length)-D1692N-2a-mRuby3-HBBIVS2-WPRE

    Plasmid
    #248993
    Purpose
    This plasmid expresses the BRCA1 sequence with D1692N mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
    Depositor
    Georgios Karras
    Article
    Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086
    Insert
    BRCA1 (BRCA1 Human)
    Use
    CRISPR
    Tags
    3x-FLAG-V5
    Mutation
    D1692N
    Available Since
    Jan. 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    248993-plasmid-map-sequence-id-495723

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  25. pKlab1154-HBEGF-2a-3xFLAG-BRCA1(full-length)-D1692H-2a-mRuby3-HBBIVS2-WPRE

    Plasmid
    #248994
    Purpose
    This plasmid expresses the BRCA1 sequence with D1692H mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
    Depositor
    Georgios Karras
    Article
    Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086
    Insert
    BRCA1 (BRCA1 Human)
    Use
    CRISPR
    Tags
    3x-FLAG-V5
    Mutation
    D1692H
    Available Since
    Jan. 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    248994-plasmid-map-sequence-id-495726

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  26. pKlab1155-HBEGF-2a-3xFLAG-BRCA1(full-length)-D1739V-2a-mRuby3-HBBIVS2-WPRE

    Plasmid
    #248995
    Purpose
    This plasmid expresses the BRCA1 sequence with D1739V mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
    Depositor
    Georgios Karras
    Article
    Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086
    Insert
    BRCA1 (BRCA1 Human)
    Use
    CRISPR
    Tags
    3x-FLAG-V5
    Mutation
    D1739V
    Available Since
    Jan. 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    248995-plasmid-map-sequence-id-497794

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  27. pKlab1156-HBEGF-2a-3xFLAG-BRCA1(full-length)-M1783T-2a-mRuby3-HBBIVS2-WPRE

    Plasmid
    #248996
    Purpose
    This plasmid expresses the BRCA1 sequence with M1783T mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
    Depositor
    Georgios Karras
    Article
    Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086
    Insert
    BRCA1 (BRCA1 Human)
    Use
    CRISPR
    Tags
    3x-FLAG-V5
    Mutation
    M1783T
    Available Since
    Jan. 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    248996-plasmid-map-sequence-id-495731

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  28. pKlab897-HBEGF-2a-3xFLAG-BRCA1(full-length)-2a-mRuby3-HBBIVS2-WPRE

    Plasmid
    #248988
    Purpose
    This plasmid expresses the BRCA1 protein sequence, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines
    Depositor
    Georgios Karras
    Article
    Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086
    Insert
    BRCA1 (BRCA1 Human)
    Use
    CRISPR
    Tags
    3x-FLAG-V5
    Available Since
    Jan. 21, 2026
    Availability
    Academic Institutions and Nonprofits only
    248988-plasmid-map-sequence-id-495729

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  29. pSA072 Cas9-HBB_IVS2-BFP

    Plasmid
    #249133
    Purpose
    Overexpression of SpCas9-BFP with HBB IVS2 intron after Cas9 coding sequence and blasticidin resistance gene. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    Cas9-HBB_IVS2-TagBFP (HBB S. pyogenes Cas9, synthetic, Human)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HBB IVS2 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249133-plasmid-map-sequence-id-496443

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  30. CD8a-AA347-363- RVEP-4A-GFP

    Plasmid
    #221597
    Purpose
    Expresses CD8a with of Arl13b ciliary targeting sequence (CTS) from amino acids 347-363 with RVEP4A mutation fused to the cytosolic tail and GFP-tagged
    Depositor
    Lei Lu
    Article
    Mahajan et al J Biol Chem. 2023 May;299(5):104604. doi
    Insert
    Arl13B (ARL13B Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Amino acids 347-363 are inserted with mutation of…
    Promoter
    CMV promoter
    Available Since
    Dec. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    221597-plasmid-map-sequence-id-494682

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Showing: 541 - 570 of 13763 results