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pSLQ2818_Blasti_pPB: CAG-PYL1-KRAB-IRES-Blasti-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9-FKBP_F36V

Plasmid

#187959

Purpose

Expressed ABA-inducible dimerizing KRAB-dCas9 system, with KRAB-IRES-Blasticidin resistance under CAG promoter and tagBFP-dCas9-FKBP12 (F36V mutant) degron under PGK promoter in a piggyBac plasmid.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Inserts

Blasticidin resistance
FKBP12_F36V

Use

CRISPR and Synthetic Biology

Tags

GGS linker

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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mcherry-p62 Double Mutation/Silent

Plasmid

#187982

Purpose

K7A/D69A p62 double mutant (PB1AA). Internal reference: SMC532

Depositor

Sascha Martens

Article

Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

Insert

p62 (NUP62 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

D7A, D69A, silent mutation

Promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSLS.416

Plasmid

#187207

Purpose

Expresses retron Eco1 ncRNA v35 (long a1/a2), and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 ncRNA v35 (long a1/a2)
Cas1 + Cas2

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7/Lac

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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GST-3C-FIP200-GFP

Plasmid

#187832

Purpose

Plasmid for the expression and purification of GST-3C-FIP200-GFP. Internal reference: SMC1445

Depositor

Sascha Martens

Article

Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

Insert

FIP200 (RB1CC1 Human)

Use

Tags

GST and mGFP

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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LV-tetO-EF1a-GFP

Plasmid

#187645

Purpose

Lentiviral vector expressing GFP from EF1a promoter downstream from tetO sites

Depositor

Martin Carroll

Insert

GFP

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac_Dual_GST-TEV-EGFP-TBK1

Plasmid

#187830

Purpose

Plasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Use

Tags

GST and TEV-mEGFP-GG Linker

Expression

Bacterial and Insect

Mutation

Promoter

Available since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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Phat2-hs-cPla2-C2-EGFP-GH

Plasmid

#187088

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSG00000116711 (PLA2G4A Human)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD33(A-N)

Plasmid

#187084

Purpose

Medium expression of Complex I with subunit N-HA tagged

Depositor

Steven Vik

Article

Zhang et al BBA Adv. 2021;1. doi: 10.1016/j.bbadva.2

Insert

nuoA-N

Use

Tags

HA tag nuoN

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBAD33(EFG)

Plasmid

#187087

Purpose

Medium expression of subunits EFG

Depositor

Steven Vik

Article

Zhang et al BBA Adv. 2021;1. doi: 10.1016/j.bbadva.2

Insert

nuoEFG

Use

Tags

HA tag nuoG

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLL5.0 shCAV1-NLS-mCherry

Plasmid

#187257

Purpose

Lentiviral expression of shRNA targeting Caveolin1 and NLS-mCherry

Depositor

Alpha Yap

Article

Katsuno-Kambe et al Biol Cell. 2021 Feb;113(2):107-117. doi:

Insert

ShRNA for Caveolin-1 (Cav1 Mouse)

Use

Lentiviral

Tags

NLS-mCherry

Expression

Mutation

Promoter

U6

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pSLPB2B-SMASh-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

Plasmid

#187949

Purpose

SMASh degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Insert

SMASh-SpdCas9-tagRFPt-P2A-tagBFP

Use

CRISPR and Synthetic Biology

Tags

GGS linker

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC H-Ras V12

Plasmid

#18741

Depositor

Scott Lowe

Article

Serrano et al Cell. 1997 Mar 7. 88(5):593-602.

Insert

H-Ras V12 (HRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

V12

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
EGFP-Tau(T217A)

Plasmid

#187025

Purpose

Expresses EGFP tagged human 2N4R tau (with T217A mutation) in mammalian cells with CMV promoter

Depositor

George Bloom

Article

Rajbanshi et al Alzheimers Dement. 2023 Jan 12. doi: 10.

Insert

MAPT (MAPT Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Changed Threonine 217 to Alanine

Promoter

CMV

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV eIF4E E103A IRES GFP

Plasmid

#18766

Depositor

Scott Lowe

Article

Wendel et al Genes Dev. 2007 Dec 15. 21(24):3232-7.

Insert

eIF4E E103A (Eif4e Mouse)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

E103A

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pDEST_FLAG-HUWE1_BH3-4E

Plasmid

#187144

Purpose

mammalian cell expression of FLAG-HUWE1 V1976E/V1780E/L1983E/M1987E

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

1xFLAG

Expression

Mammalian

Mutation

V1976E/V1780E/L1983E/M1987E

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_delta3291-3298

Plasmid

#187125

Purpose

mammalian cell expression of FLAG-HUWE1 delta3291-3298

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

1xFLAG

Expression

Mammalian

Mutation

delta3291-3298

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pSBK.013

Plasmid

#187213

Purpose

Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 5 [ACG-CGT]), and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 ncRNA v35 (long a1/a2, barcode 5 [ACG-CGT])
Cas1 + Cas2

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7/Lac

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_C_DUX4

Plasmid

#187799

Purpose

MAC-tagged gene expression of human DUX4

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

DUX4 (DUX4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_C_EPHB6

Plasmid

#187763

Purpose

MAC-tagged gene expression of human EPHB6

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

EPHB6 (EPHB6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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RFP-FKBP-U2AF35

Plasmid

#187969

Purpose

expresses U2AF35 with N-terminal tags RFP and FKBP

Depositor

Markus Wahl

Article

Vester et al Commun Biol. 2022 Jul 22;5(1):736. doi:

Insert

U2AF35 (U2af1 Mouse)

Use

Cmv promoter

Tags

mRFP-FKBP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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