We narrowed to 624 results for: DST;

1. pcDNA3.1(+)/(CAA)12 + (AGAGAG)7 5′ leader AUG-nLuc-3XFLAG

   Plasmid

   #127326

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTR

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. p2R3a-nosT

   Plasmid

   #71272

   Purpose

   Entry clone containing nosT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

   Insert

   nopaline synthase terminator
   

   Use

   Gateway

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. p2R3a-TagRFP-OcsT

   Plasmid

   #71270

   Purpose

   Entry clone containing TagRFP. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

   Insert

   TagRFP
   

   Use

   Gateway

   Tags

   4xGly linker and octaline synthase terminator

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. JpExpress404 PTEN-LONG

   Plasmid

   #49417

   Purpose

   Bacterial Expression Vector

   Depositor

   Ramon Parsons

   Article

   Hopkins et al Science. 2013 Jul 26;341(6144):399-402.

   Insert

   PTEN-Long (PTEN )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available Since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti_Cas9_T2A_mNeonGreen_P2A_blasticidin

   Plasmid

   #211471

   Purpose

   lentiviral tricistronic complex expressing Cas9, mNeonGreen, and blasticidin resistance

   Depositor

   James Eshleman

   Article

   Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

   Insert

   Cas9_T2A_mNeonGreen_P2A_BlasticidinR
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, EF-1a

   Available Since

   March 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBC2T-LC3B

   Plasmid

   #112026

   Purpose

   expresses BC2-tag-LC3B in mammalian cells

   Depositor

   Ulrich Rothbauer

   Article

   Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

   Insert

   LC3B (MAP1LC3B Human)
   

   Use

   Tags

   BC2-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. 3XFlag pCMV5-TOPO TAZ (S89A)

   Plasmid

   #24815

   Depositor

   Jeff Wrana

   Article

   Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

   Insert

   TAZ (WWTR1 Human)
   

   Use

   Tags

   3XFlag

   Expression

   Mammalian

   Mutation

   Serine 89 mutated to Alanine

   Promoter

   Available Since

   May 14, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. 3XFlag pCMV5-TOPO TAZ (∂WW)

   Plasmid

   #24811

   Depositor

   Jeff Wrana

   Article

   Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

   Insert

   TAZ (WWTR1 Human)
   

   Use

   Tags

   3XFlag

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 111-158.

   Promoter

   Available Since

   May 14, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pJW1839

   Plasmid

   #154325

   Purpose

   sgRNA Destination Vector for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   SapTrap sgRNA (F+E) vector, R07E5.16 U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   F+E mutations in sgRNA

   Promoter

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. TV-hPITX3-forward

    Plasmid

    #22073

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

    Insert

    PITX3 (ASMD Human)
    

    Use

    Cre/Lox; Zfn donor

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3XFlag pCMV5-TOPO TAZ ∂CC

    Plasmid

    #24816

    Depositor

    Jeff Wrana

    Article

    Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

    Insert

    TAZ (WWTR1 Human)
    

    Use

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 222-265 (coiled coil doma…

    Promoter

    Available Since

    May 14, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

12. 3XFlag pCMV5-TOPO TAZ (∂304)

    Plasmid

    #24810

    Depositor

    Jeff Wrana

    Article

    Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

    Insert

    TAZ (WWTR1 Human)
    

    Use

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    Contains ONLY amino acids 1-304.

    Promoter

    Available Since

    June 3, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

13. p2R3a-3AT

    Plasmid

    #71273

    Purpose

    Entry clone containing 3AT. Necessary to complete the transcriptional reporters by providing the poly-A tail. For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

    Insert

    T3A ribulose-1,5-bisphosphate carboxylase 3A subunit terminator
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. 3XFlag pCMV5-TOPO TAZ (106∂)

    Plasmid

    #24814

    Depositor

    Jeff Wrana

    Article

    Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

    Insert

    TAZ (WWTR1 Human)
    

    Use

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    Deletion of aa 1-106

    Promoter

    Available Since

    May 14, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pU6-Universal.Sth.dCas9

    Plasmid

    #171088

    Purpose

    CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.

    Depositor

    Sebastian Lourido

    Article

    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

    Inserts

    Toxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
    DHFR-TSc3
    

    Use

    Toxoplasma gondii expression

    Tags

    Expression

    Mutation

    Note: A S245F mutation is present but did not app…

    Promoter

    DHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV_-CMV-Pcdh15_mini_V4-CD2

    Plasmid

    #199184

    Purpose

    An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

    Depositor

    David Corey

    Article

    Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

    Insert

    mmPcdh15_mini_V4-CD2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    EC4, EC5, EC6, EC7, EC8 are deleted from msPCDH15…

    Promoter

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJW1582

    Plasmid

    #154312

    Purpose

    mNeonGreen^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    mNeonGreen^SEC^AID*::3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-TUBG2

    Plasmid

    #171966

    Purpose

    Expression in cell lines of the TUBG2 gene

    Depositor

    Maria Alvarado-Kristensson

    Article

    Corvaisier et al Commun Biol. 2021 Jun 22;4(1):767. doi:

    Insert

    TUBG2 (TUBG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Removal of the internal TUBG2-EcoRI site by chang…

    Promoter

    Available Since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBC2T-lamin

    Plasmid

    #108972

    Purpose

    expresses BC2-tag-lamin in mammalian cells

    Depositor

    Ulrich Rothbauer

    Article

    Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

    Insert

    lamin B1 (LMNB1 Human)
    

    Use

    Tags

    BC2-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJW2088

    Plasmid

    #154336

    Purpose

    Crispr repair template: add insertion site specific homology arms by PCR before insertion

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::GFP::3xFLAG::10aa linker
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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