We narrowed to 1,037 results for: David Sabatini

1. pLKO human shRNA 2 c-MAF

   Plasmid

   #29433

   Depositor

   David Sabatini

   Article

   Peterson et al Cell. 2009 May 13. ():.

   Insert

   c-MAF shRNA 2 (MAF Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 7, 2011

   Availability

   Academic Institutions and Nonprofits only

2. DPYS-FLAG

   Plasmid

   #61618

   Purpose

   To study the effect of DPYS activity on EMT

   Depositor

   David Sabatini

   Article

   Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

   Insert

   dihydropyrimidinase (DPYS Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HA Raptor mutant 3

   Plasmid

   #8473

   Depositor

   David Sabatini

   Article

   Kim et al Cell 2002 Jul 26;110(2):163-75.

   Insert

   Raptor (RPTOR Human)
   

   Use

   Tags

   ha

   Expression

   Mammalian

   Mutation

   313NWIF316-AAAA (N313A, W314A, I315A, F316A)

   Promoter

   Available since

   May 11, 2005

   Availability

   Academic Institutions and Nonprofits only

4. pLJM60 FLAG NUFIP1 W40A

   Plasmid

   #110951

   Purpose

   expresses mammalian FLAG-NUFIP1 W40A

   Depositor

   David Sabatini

   Article

   Wyant et al Science. 2018 May 18;360(6390):751-758.

   Insert

   NUFIP1 W40A (NUFIP1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Tryptophan 40 to alanine

   Promoter

   CMV

   Available since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HA GST F5A deltaCT S6K1 pRK5

   Plasmid

   #8467

   Depositor

   David Sabatini

   Article

   Ali et al J Biol Chem 2005 Apr 4;.

   Insert

   f5a delta CT p85 s6k1 (Rps6kb1 Rat)
   

   Use

   Tags

   ha/gst

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

6. plentiCRISPR-sgMDH1

   Plasmid

   #72875

   Purpose

   The lentiviral sgMDH1 vectors was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB

   Depositor

   David Sabatini

   Article

   Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

   Insert

   sgMDH1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pLJC6-Flag CASTOR1 I280A

   Plasmid

   #84497

   Purpose

   Lentiviral expression of CASTOR2 I280A mutant

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

   Insert

   CASTOR1 I280A (GATSL3 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   I280A

   Promoter

   sestrin2

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

8. delta NT delta CT p70S6K1 MSCV puro

   Plasmid

   #8463

   Depositor

   David Sabatini

   Article

   Ali et al J Biol Chem 2005 Apr 4;.

   Insert

   delta NT delta CT p70 S6K1 (Rps6kb1 Rat)
   

   Use

   Retroviral

   Tags

   myc

   Expression

   Mammalian

   Mutation

   p70 s6k1 form

   Promoter

   Available since

   June 20, 2005

   Availability

   Academic Institutions and Nonprofits only

9. lentiCRISPR - Amplicon, JAK2 sgRNA 1

   Plasmid

   #70679

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic JAK2 amplicon-targeting sgRNA element from U6 promoter. Lentiviral backbone

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against JAK2 amplicon found in AML-derived HEL cell line (JAK2 )
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

10. deltaCT S6K1 MSCV puro

    Plasmid

    #8465

    Depositor

    David Sabatini

    Article

    Ali et al J Biol Chem 2005 Apr 4;.

    Insert

    delta ct p70 S6K1 (Rps6kb1 Rat)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mammalian

    Mutation

    deletion of 104 c-terminal amino acids

    Promoter

    Available since

    May 11, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pMXs_FLAG-MCART1W283A

    Plasmid

    #138409

    Purpose

    Express FLAG-tagged MCART1W283A in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    SLC25A51 (SLC25A51 Human)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells, W2…

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMXs_FLAG-ODC2

    Plasmid

    #133257

    Purpose

    Express FLAG-tagged ODC2 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    ODC2 (ODC2 Budding Yeast)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMXs_FLAG-NDT2

    Plasmid

    #133255

    Purpose

    Express FLAG-tagged NDT2 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

    Insert

    NDT2 (YEA6 Budding Yeast)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    codon-optimized for expression in human cells

    Promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRK5-FLAG-FLCN (C terminus)

    Plasmid

    #72296

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

    Insert

    FLCN C-terminus (FLCN Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains only C terminus of FLCN as defined in PM…

    Promoter

    CMV

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pLentiCRISPR sgHAL

    Plasmid

    #102315

    Purpose

    genetic depletion of HAL

    Depositor

    David Sabatini

    Article

    Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    sgHAL (HAL Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLJC1-Rap2A-3xFLAG

    Plasmid

    #87972

    Purpose

    Expression of Rap2A in mammalian cells

    Depositor

    David Sabatini

    Article

    Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pWP1_mFTCD

    Plasmid

    #102317

    Purpose

    expression of mouse cDNA of FTCD, refractory to the guides in plasmids 1,2

    Depositor

    David Sabatini

    Article

    Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    Ftcd (Ftcd Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1alpha

    Available since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LentiCRISPR-sgC1orf27-1

    Plasmid

    #86130

    Purpose

    Lentiviral vector expressing Cas9 and an sgRNA targeting C1orf27

    Depositor

    David Sabatini

    Article

    Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

    Insert

    sgRNA 1 targeting C1orf27 (C1orf27 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRK5-FLAG-SZT2 2241-end

    Plasmid

    #87039

    Purpose

    mammalian expression of SZT2 fragment

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    SZT2 (SZT2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AA 2241-end

    Promoter

    CMV

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRK5-FLAG-SZT2 1-1021

    Plasmid

    #87036

    Purpose

    mammalian expression of SZT2 fragment

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    SZT2 (SZT2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AA 1-1021

    Promoter

    CMV

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge