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pFUW-TetO-NMI

Plasmid

#178446

Purpose

doxycycline-inducible expression of Human NMI in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

NMI (NMI Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCas9D10A_GFP

Plasmid

#44720

Purpose

Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells

Depositor

Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

Insert

Cas9D10A-2A-GFP

Use

CRISPR

Tags

2A-GFP

Expression

Mammalian

Mutation

D10A mutation- nickase mutant

Promoter

CAG

Available since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pSBbi 41BBL CD86 HLA-A201

Plasmid

#183248

Purpose

Expression of human 41BBL, CD86 and HLA-A201 in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

Depositor

Alexander McLellan

Article

Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

Inserts

CD86, 41BBL
HLA-A2 (HLA-A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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FU-tet-o-hc-myc

Plasmid

#19775

Depositor

Konrad Hochedlinger

Article

Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

Insert

c-myc (MYC Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

T58A

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-hMYC

Plasmid

#20723

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

Insert

c-MYC (MYC Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

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FU-tet-o-hKLF4

Plasmid

#19777

Depositor

Konrad Hochedlinger

Article

Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

Insert

Klf4 (KLF4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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FU-tet-o-hOct4

Plasmid

#19778

Depositor

Konrad Hochedlinger

Article

Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

Insert

Oct4 (POU5F1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pCCLc-MNDU3-Luciferase-PGK-EGFP-WPRE

Plasmid

#89608

Purpose

Expression of Luciferase in human cells. 3rd generation lentiviral vector

Depositor

Fernando Fierro

Article

Beegle et al Stem Cells. 2015 Jun;33(6):1818-28. doi:

Insert

Luciferase

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

MNDU3

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-hOCT4

Plasmid

#20726

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

Insert

OCT4 (POU5F1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-cMYC

Plasmid

#20324

Depositor

Rudolf Jaenisch

Article

Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

Insert

cMYC (Myc Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-hKLF4

Plasmid

#20725

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

Insert

KLF4 (KLF4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

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Nanog-2A-GFP

Plasmid

#59994

Purpose

Inserts GFP immediately downstream of the Nanog coding region

Depositor

Rudolf Jaenisch

Article

Faddah et al Cell Stem Cell. 2013 Jul 3;13(1):23-9. d

Insert

Nanog (Nanog Mouse)

Use

Mouse Targeting

Tags

2A-GFP

Expression

Mutation

Promoter

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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Nanog-2A-mCherry

Plasmid

#59995

Purpose

Inserts mCherry immediately downstream of the Nanog coding region

Depositor

Rudolf Jaenisch

Article

Faddah et al Cell Stem Cell. 2013 Jul 3;13(1):23-9. d

Insert

Nanog (Nanog Mouse)

Use

Mouse Targeting

Tags

2A-mCherry

Expression

Mutation

Promoter

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pLOVE-Nmyc

Plasmid

#15951

Depositor

Miguel Ramalho-Santos

Article

Blelloch et al Cell Stem Cell. 2007 Sep 13. ():.

Insert

v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian) (Mycn Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

The recombination reaction required to clone &quo…

Promoter

Available since

Nov. 7, 2007

Availability

Academic Institutions and Nonprofits only
TetO-FUW-oct4

Plasmid

#20323

Depositor

Rudolf Jaenisch

Article

Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

Insert

oct4 (Pou5f1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-Sox1

Plasmid

#61545

Purpose

Expresses Sox1 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

Sox1 (Sox1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 14, 2015

Availability

Academic Institutions and Nonprofits only
GFP-mTSP15

Plasmid

#236542

Purpose

For expression of Tspan15 in mammalian cells

Depositor

Sandra Citi

Article

Shah et al Cell Rep. 2018 Nov 20;25(8):2132-2147.e7

Insert

TSPAN15 (Tspan15 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-mTSP33

Plasmid

#236541

Purpose

For expression of Tspan33 in mammalian cells

Depositor

Sandra Citi

Article

Shah et al Cell Rep. 2018 Nov 20;25(8):2132-2147.e7

Insert

TSPAN33 (Tspan33 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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H2B-mCherry

Plasmid

#20972

Purpose

H2B fused to mCherry for chromatin visualization

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

Histone cluster 1, H2bb (H2BC3 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 7, 2009

Availability

Academic Institutions and Nonprofits only

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CMV-GFP-NMHC II-B

Plasmid

#11348

Depositor

Robert Adelstein

Article

Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27

Insert

NMHC II-B (MYH10 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 16, 2006

Availability

Academic Institutions and Nonprofits only

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