165,075 results

1. pCI-T7Max-UTR1-deGFP-8xHis-T500

   Plasmid

   #178422

   Purpose

   Expression of deGFP via a T7 Promoter in TXTL

   Depositor

   Kate Adamala

   Article

   Deich et al J Biol Eng. 2023 Jan 23;17(1):4. doi: 10

   Insert

   deGFP
   

   Use

   Synthetic Biology

   Tags

   8xHis Tag

   Expression

   Bacterial

   Promoter

   T7Max

   Available Since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSB1C3-J23110-B0034-mRFP1_Yellow

   Plasmid

   #160443

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Yellow chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

   Insert

   promoter, RBS, mRFP1E_Yellow
   

   Use

   Synthetic Biology

   Mutation

   BioBrick sites removed

   Available Since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-Tight-Puro_Nkx6.2

   Plasmid

   #64846

   Purpose

   A lentiviral vector for the expression of mouse Nkx6.2 under doxycyline control

   Depositor

   Paul Tesar

   Article

   Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

   Insert

   Nkx6.2 (Nkx6-2 Mouse)
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Available Since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNCS Citrine

   Plasmid

   #91761

   Purpose

   Bacterial expression of a fluorescent protein, Citrine

   Depositor

   Roger Tsien, Erik Rodriguez

   Article

   Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

   Insert

   Citrine
   

   Tags

   Hexa-Histidine tag, Xpress epitope for detection …

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pD649-HAsp-EDA-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156768

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   EDA (EDA Human)
   

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV6-G1-ATP8-Myc-FLAG (G418)

   Plasmid

   #86850

   Purpose

   mammalian expression of ATP8 with mitochondrial targeting sequence, Myc, and FLAG tags

   Depositor

   Matthew O'Connor

   Article

   Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

   Insert

   ATP8 (ATP8 Human)
   

   Tags

   ATP5G1 (G1) (MQTAGALFISPALIRCCTRGLIRPVSASFLNS PVN…

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   CMV

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pgLAP4

   Plasmid

   #19705

   Depositor

   Peter Jackson

   Insert

   pgLAP4
   

   Tags

   Flag, S peptide, and Tev

   Expression

   Mammalian

   Available Since

   April 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMVTO-SEAP-26Sfur-3TM-tevs-AAMP

   Plasmid

   #179633

   Purpose

   SEAP RELEASE construct with AAMP motif control

   Depositor

   Xiaojing Gao

   Article

   Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

   Insert

   SEAP RELEASE
   

   Expression

   Mammalian

   Available Since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF1842

   Plasmid

   #143324

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   YWHAZ (YWHAZ Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR223-TLK2

    Plasmid

    #23629

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    TLK2 (TLK2 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only