12,329 results

1. pGL2_137

   Plasmid

   #199084

   Purpose

   Even ORI-marker screen destination vector with sfYFP dropout for barcoded-ORI insertion

   Depositor

   Charlie Gilbert, Nili Ostrov, Cultivarium Tools

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL1_36

   Plasmid

   #199043

   Purpose

   Level 1 plasmid, barcoded ORI

   Depositor

   Charlie Gilbert, Nili Ostrov, Cultivarium Tools

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   pBBR1 ORI plus NGS barcode 13
   

   Use

   Synthetic Biology

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFD-FRB

   Plasmid

   #137107

   Purpose

   iFLinkC Linker cloning

   Depositor

   Viktor Stein

   Article

   Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

   Insert

   FRB
   

   Use

   Synthetic Biology

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRF-HapR

   Plasmid

   #49360

   Depositor

   Christopher Voigt

   Article

   Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

   Insert

   HapR repressor
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRF-SmcR

   Plasmid

   #49371

   Depositor

   Christopher Voigt

   Article

   Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

   Insert

   SmcR repressor
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBR322-bom

   Plasmid

   #155178

   Purpose

   pBICI_2_strong without bom site

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

   Inserts

   Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
   Cellobiose phosphorylase from Cellulomonas uda
   

   Use

   Synthetic Biology

   Tags

   His Tag and Strep - Tag II

   Expression

   Bacterial

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBW2653_pCAG-PV1-NTD-ENTRY-L1-nMag-NLS-BGHpA

   Plasmid

   #108846

   Purpose

   Insert protein fragment into MluI/BspEI to fuse to nMag/NLS tags on C-terminus

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   nMag,NLS

   Expression

   Mammalian

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPD355 VPR-FRB (NLS)

   Plasmid

   #138847

   Purpose

   Constitutive expression of VPR-FRB under the CMV promoter.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   VPR-FRB
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFTK080

   Plasmid

   #171352

   Purpose

   Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

   Depositor

   Arnold Driessen

   Article

   Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

   Insert

   Tcyc1 YJR048W terminator
   

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Mutation

   n/a

   Available Since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFTK079

    Plasmid

    #171351

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    Toat1 Pc18g03600 terminator
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Available Since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFTK052

    Plasmid

    #171324

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    Firefly luciferase reporter
    

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBbE2k-RFP

    Plasmid

    #35324

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Tet

    Available Since

    Feb. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pALxR1

    Plasmid

    #172564

    Purpose

    Inducible gene expression, pACYC origin, LuxR activator, PLuxB promoter, mRFP, Chloramphenicol Resistance Marker.

    Depositor

    Christopher Reisch

    Insert

    LuxR-mRFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PLuxB

    Available Since

    Nov. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. MADR pDonor TagBFP2-V5-HRAS G12V WPRE RCE

    Plasmid

    #131730

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion compatible with RCE:loxP mouse strain

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    TagBFP2-V5-HRAS G12V (HRAS Entacmaea quadricolor (TagBFP), Human)
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    TagBFP2-V5 tag

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. p148

    Plasmid

    #62616

    Purpose

    ePathBrick pETM6 vector encoding CsF3H, AaDFR, and DuLAR

    Depositor

    Mattheos Koffas

    Article

    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

    Inserts

    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    

    Use

    Synthetic Biology

    Tags

    GBD, PDZ, and SH3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL2_177

    Plasmid

    #199109

    Purpose

    Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

    Depositor

    Charlie Gilbert, Nili Ostrov, Cultivarium Tools

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    pBC1 ORI plus NGS barcode 25, gentamicin resistance cassette, mScarlet-I cassette, oriT
    

    Use

    Synthetic Biology

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGB 35S:dCas9:Tnos (GB1191)

    Plasmid

    #68223

    Purpose

    Transcriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    dCas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed; human codon optimis…

    Promoter

    35S

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-TM-Kv2.1-CaM-NES-TevN-AsLOV2-TEVseq-tTA

    Plasmid

    #171616

    Purpose

    Soma-targeted Cal-Light with Kv2.1

    Depositor

    Hyungbae Kwon

    Article

    Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

    Insert

    TM-Kv2.1-CaM-NES-TevN-AsLOV2-TEVseq-tTA
    

    Use

    Synthetic Biology

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. XLone-BSD ID2

    Plasmid

    #179513

    Purpose

    Tunable and temporal expression control of ID2

    Depositor

    Xiaoping Bao

    Article

    Jung et al ACS Synth Biol. 2022 May 24. doi: 10.102

    Insert

    ID2 (ID2 Human)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3GS Promoter

    Available Since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKS044

    Plasmid

    #65472

    Purpose

    pTrc plasmid expressing clpP

    Depositor

    Keith Tyo

    Article

    Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

    Insert

    clpP (clpP )
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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