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pDarmo.CMVT_v1

Plasmid

#133072

Purpose

Entry vector for cloning genes under an enhanced CMVT promoter for expression in mammalian cells. Version 1.0.

Depositor

Kacper Rogala, David Sabatini

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Gateway Cloning

Tags

3C-Flag-TwinStrep and TwinStrep-Flag-3C

Expression

Mammalian

Promoter

CMVT (Enhanced CMV)

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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Hes5-Luc

Plasmid

#41724

Depositor

Ryoichiro Kageyama, Raphael Kopan

Article

Nishimura et al Genomics. 1998 Apr 1;49(1):69-75.

Insert

Hes5 Promoter (Hes5 Mouse)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Contains the murine Hes5 promoter (-800 to +73)

Promoter

Hes5

Available Since

Feb. 1, 2013

Availability

Academic Institutions and Nonprofits only
pRK5 DN-Fyn

Plasmid

#16033

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

c-Fyn (FYN Human)

Expression

Mammalian

Mutation

Dominant negative: K299M

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pIRES_IBB-mCherry-IRES-HA-mAID-nanobody

Plasmid

#117720

Purpose

Expression of nuclear transfection marker IBB-mCherry and HA-tagged mAID-nanobody from one mRNA

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Inserts

mAID-nanobody
IBB-mCherry

Tags

importin-beta bining domain (IBB) and triple HA

Expression

Mammalian

Mutation

codon optimized mAID for murine expression

Promoter

CMV and None, IRES-driven

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE167

Plasmid

#118515

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLAG-APEX2-MAP1LC3B

Plasmid

#175753

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-MAP1LC3B

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

MAP1LC3B (MAP1LC3B Human)

Use

Lentiviral

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2+ EB3-mKate2

Plasmid

#105940

Purpose

in vitro transcription

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

EB3 (MAPRE3 Human)

Tags

mKate2

Expression

Bacterial

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-160

Plasmid

#118572

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pRedCmOcr

Plasmid

#176226

Purpose

A helper plasmid for expression of the artificial T7ocr-gam-bet-exo operon. The plasmid carries the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage.

Depositor

Sergey Sineoky

Article

Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

Insert

Lambda Red-T7ocr operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage

Use

Synthetic Biology

Expression

Bacterial

Promoter

PrhaB and PL

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-FLNa S2152A

Plasmid

#8983

Depositor

John Blenis

Article

Woo et al Mol Cell Biol. 2004 Apr . 24(7):3025-35.

Insert

Filamin A S2152A (FLNA Human)

Tags

myc

Expression

Mammalian

Mutation

Ser2152Ala

Available Since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry-GFP-nb

Plasmid

#238259

Purpose

For overexpression of mCherry-GFP-nb

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry-GFP-nb

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_VHH05-nb-KS

Plasmid

#238270

Purpose

For overexpression of VHH05-nb-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

VHH05-nb-KS

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_ALFA-nb-KS

Plasmid

#238263

Purpose

For overexpression of ALFA-nb-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

ALFA-nb-KS

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_VHH05-nb-KS-FtoG

Plasmid

#238271

Purpose

For overexpression of VHH05-nb-KS-FtoG

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

VHH05-nb-KS-FtoG

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_VHH05-nb-noKS

Plasmid

#238269

Purpose

For overexpression of VHH05-nb-noKS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

VHH05-nb-noKS

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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EYFP-JAB1

Plasmid

#111213

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Orel et al J Cell Mol Med. 2010 Jun;14(6B):1555-68.

Insert

CSN5 (COPS5 Human)

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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LTN1_pCSdest

Plasmid

#53855

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

LTN1 (LTN1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

Sept. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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bdSUMO-ZF5.3-GFP-HiBit

Plasmid

#170992

Purpose

For bacterial expression of bdSUMO tagged muGFP with ZF5.3 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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GST-BHMT-I/G

Plasmid

#104442

Purpose

Autophagy cargo assay

Depositor

Carol Mercer

Article

Mercer et al Autophagy. 2008 Feb;4(2):185-94. Epub 20

Insert

BHMT (BHMT Human)

Tags

GFP-myc GFP is from Aequorea victoria and GST

Expression

Mammalian

Mutation

N43A in GST (in a potential KFERQ-like sequence).…

Promoter

CMV

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-Adrm1

Plasmid

#19417

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

Insert

adhesion regulating molecule 1 (ADRM1 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pMRE-Tn7-166

Plasmid

#118578

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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CAV1-K*R-mEGFP

Plasmid

#27766

Depositor

Ari Helenius

Article

Hayer et al J Cell Biol. 2010 Nov 1. 191(3):615-29.

Insert

caveolin-1, lysine null mutant (CAV1 Human)

Tags

monomeric EGFP

Expression

Mammalian

Mutation

All lysines mutated to arginines (K*R)

Available Since

Aug. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-156

Plasmid

#118570

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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TMPRSS3_pCSdest

Plasmid

#53839

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

TMPRSS3 (TMPRSS3 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
ADAMTS5_pCSdest

Plasmid

#53819

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

ADAMTS5 (ADAMTS5 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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bdSUMO-TAT-GFP-HiBit

Plasmid

#170991

Purpose

For bacterial expression of bdSUMO tagged muGFP with TAT peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-TAT-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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mouse CENP-B-EGFP

Plasmid

#107264

Purpose

mouse CENP-B-EGFP in pIVT vector for in vitro transcription

Depositor

Michael Lampson

Article

Akera et al Science. 2017 Nov 3;358(6363):668-672. d

Insert

CENP-B-EGFP (Cenpb Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

T7

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMDJ113 - pEXP(HygroR, 5605)[Phsp-16.41 |Cre recombinase (PATCs) | tbb-2 UTR]

Plasmid

#159809

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP(HygroR, 5605)[Phsp-16.41 |Cre recombinase (PATCs) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-SEMPER_ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

Plasmid

#221075

Purpose

SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

Depositor

Mikhail Shapiro

Article

Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

Insert

ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-155

Plasmid

#118537

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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