We narrowed to 9,372 results for: bli

1. pCDB179-LCC

   Plasmid

   #218686

   Purpose

   Encodes codon optimized LCC enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

   Insert

   LCC
   

   Tags

   10xHis

   Expression

   Bacterial

   Available Since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. 2xMARS-nGFP

   Plasmid

   #205235

   Purpose

   Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells

   Depositor

   Jeremy Baskin

   Insert

   2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
   

   Tags

   HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

   Expression

   Mammalian

   Mutation

   amino acids 143-271 of PLEKHA5 (GenBank reference…

   Promoter

   CMV

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-sCAG-eGFP-RAB11

   Plasmid

   #203799

   Purpose

   AAV vector plasmid expressing human RAB11 fused to eGFP under a short variant of the CAG (sCAG) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis

   Insert

   RAB11 (RAB11A Human)
   

   Use

   AAV

   Tags

   eGFP

   Expression

   Mammalian

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-PGK-CXCR4 (neo)

   Plasmid

   #192077

   Purpose

   Lentivirus for expression of CXCR4

   Depositor

   Roland Friedel

   Insert

   CXCR4 (CXCR4 Human)
   

   Use

   Lentiviral

   Promoter

   hPGK

   Available Since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOTTC1761 - pAAV SYN1 DIO HA-hM3D(Gq)

   Plasmid

   #125147

   Purpose

   An adeno-associated viral vector expressing Cre-dependent HA-tagged inhibitory DREADD (hM3D(Gq)) from a synapsin promoter

   Depositor

   Christopher Richie

   Insert

   HA-tagged hM3D(Gq) (CHRM3 Human)
   

   Use

   AAV and Cre/Lox

   Tags

   HA

   Expression

   Mammalian

   Promoter

   SYN1

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLTR-SFFV-PTPN2 transcript variant 1-WPRE-UbC-Emerald

   Plasmid

   #201186

   Purpose

   Lentiviral vector plasmid expressing human PTPN2 transcript variant 1 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Bart Nieuwenhuis

   Inserts

   PTPN2, transcript variant 1 (PTPN2 Human)
   Emerald
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pdCas9-NED

   Plasmid

   #109358

   Purpose

   Mammalian vector for expressing effector domains fused to dCas9.

   Depositor

   Antal Kiss

   Article

   Goubert et al Methods Mol Biol. 2018;1767:395-415. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   dCas9, SV40 NLS, 3xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Mouse 3' Hp1a AID GFP PuroR

   Plasmid

   #127897

   Purpose

   HDR (donor) Plasmid for Inserting AID-GFP-2A-Puro into the 3' end of the Mouse Hp1a Gene

   Depositor

   Mark Groudine

   Insert

   Hp1a (Cbx5 Mouse, Mustard Weed)
   

   Use

   Donor plasmid

   Tags

   GFP, AID, PuroR

   Mutation

   Inserting GFP AID 2A Puro into mouse 3' Hp1a

   Available Since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. mApple-hCOL1A1

   Plasmid

   #140113

   Purpose

   Expresses fluorescently-tagged human Type I procollagen α1 chain with mApple replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

   Depositor

   Sergey Leikin

   Insert

   Type I procollagen α1 (COL1A1 Human)
   

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   COL1A1 exons 2-3 replaced by fluorescent tag

   Promoter

   CMV

   Available Since

   May 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFP.R267

    Plasmid

    #138179

    Purpose

    Expresses the split mCherry reporter interrupted by the gp41-1 intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(SGY).gp41-1.(SSS)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    P(araBAD) and iPC

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-UBC9-K14R-A129K

    Plasmid

    #228948

    Purpose

    express a fragment of the double mutant of the human UBC9 (generated from pET28-UBC9 through mutagenesis; point mutations: K14R and A129K)

    Depositor

    Marcin Suskiewicz

    Insert

    UBC9 (UBE2I Human)
    

    Tags

    His tag and thrombine cleavage site : MGHHHHHHSSG…

    Expression

    Bacterial

    Mutation

    changed Lysine 14 to Arginine, changed Alanine 12…

    Promoter

    T7-lac

    Available Since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJBJZ_003

    Plasmid

    #155358

    Purpose

    Rifampicin derivative of lambda-red pKD3 plasmid from Datsenko and Wanner (2000)

    Depositor

    Elisabeth Raleigh

    Article

    Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Sept. 30, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pFP.R377

    Plasmid

    #138247

    Purpose

    Expresses the split mCherry reporter interrupted by the Mja-KlbA intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(HDG).Mja-KlbA*.(CSG)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    P(araBAD) and iPC

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. DT12A

    Plasmid

    #80416

    Purpose

    Expresses 12 CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

    Depositor

    Thomas Cooper

    Article

    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

    Insert

    human DMPK exons 11-15 with 12 CTG repeats (DMPK Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. mVenus-hCOL1A1

    Plasmid

    #140111

    Purpose

    Expresses fluorescently-tagged human Type I procollagen α1 chain with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Insert

    Type I procollagen α1 (COL1A1 Human)
    

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    COL1A1 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available Since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQTEV-FLJ30046

    Plasmid

    #34828

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    FLJ30046 (SLAIN1 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQTEV-SCP2

    Plasmid

    #31336

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    sterol carrier protein 2 (SCP2 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. AMPKA2

    Plasmid

    #36194

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    AMPKA2 (PRKAA2 Human)
    

    Tags

    His tag with thrombin cleavage

    Expression

    Bacterial

    Mutation

    contains KINASE DOMAIN only

    Available Since

    Aug. 8, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pGL4.10/RSV_XS3B-miniXon_Luciferase

    Plasmid

    #217302

    Purpose

    Plasmid containing the SF3B3-XS3B/Firefly luciferase cassette, a modification of the SF3B3-miniXon system. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-XS3B by L

    Depositor

    Beverly L. Davidson

    Insert

    XS3B-miniXon
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Kozak ATG initiation sequence in middle exon

    Promoter

    RSV

    Available Since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. Galt1-sCGrx1p-HA

    Plasmid

    #230028

    Purpose

    Expressing sCGrx1p protein fused to HA tag in mammalian cells with localization in the Golgi apparatus.

    Depositor

    Pablo Rivera-Fuentes

    Insert

    Galt1-sCGrx1p-HA (B4GALT1 Human, Synthetic)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. pRN3P_AID-Dam-only

    Plasmid

    #136065

    Purpose

    in vitro transcription for mRNA

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Borsos et al Nature. 2019 May;569(7758):729-733. doi:

    Insert

    AID-Dam only
    

    Use

    In vitro transcription

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pQTEV-RPL9

    Plasmid

    #31334

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    ribosomal protein L9 (RPL9 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. B52_puro_empty_gRNA

    Plasmid

    #197557

    Purpose

    sites for cloning two gRNA with puromycin cassette

    Depositor

    Darius Bägli

    Type

    Empty backbone

    Use

    Empty grna backbone with puromycin resistance gene

    Expression

    Mammalian

    Promoter

    U6 for gRNA, CMV for puromycin resistance.

    Available Since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. RabV CVS-N2c(deltaG)-dsRed

    Plasmid

    #73460

    Purpose

    Expresses dsRed for monosynaptic circuit mapping

    Depositor

    Thomas Jessell

    Article

    Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

    Insert

    dsRed
    

    Use

    Neurotropic virus

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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25. RabV CVS-N2c(deltaG)-Cre

    Plasmid

    #73474

    Purpose

    Expresses Cre for circuit mapping and genetic manipulation

    Depositor

    Thomas Jessell

    Article

    Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

    Insert

    Cre recombinase
    

    Use

    Neurotropic virus

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pL1P5 ZmUbiP:GRF-GIF:NosT

    Plasmid

    #198046

    Purpose

    MoClo Golden Gate Level 1 Position 5. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.

    Depositor

    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing

    Insert

    ZmUbiP::TaGRF4-GIF1::NosT
    

    Use

    Synthetic Biology

    Promoter

    Zea mays (maize) ubiquitin promoter

    Available Since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. pLenti HsATP10B_D433N-T2A-His-flag-TMEM30A

    Plasmid

    #191676

    Purpose

    transfer plasmid for lentiviral vector production expressing Hs ATP10B D433N mutant and His/flag tagged Hs TMEM30A

    Depositor

    Veerle Baekelandt

    Inserts

    ATP10B (ATP10B Human)
    TMEM30A (TMEM30A Human)
    

    Use

    Lentiviral

    Tags

    6x HIS and flag

    Mutation

    D433N

    Available Since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCMV-T7-SpRY-P2A-mScarlet (MNW002)

    Plasmid

    #174137

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    SpG=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317…

    Promoter

    CMV and T7

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPyCAG-tagBFP-3xNLS-IRES-Hph

    Plasmid

    #183608

    Purpose

    Mammalian expression vector for tagBFP-3xNLS from the CAG promoter. Confers hygromycin B resistance.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    tagBFP-3xNLS
    

    Tags

    3xNLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLV-SFFV-ZFYVE27-WPRE-UbC-mCherry

    Plasmid

    #214470

    Purpose

    Lentiviral vector plasmid expressing human zinc finger FYVE-type containing 27 (ZFYVE27) under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis

    Inserts

    ZFYVE27 (ZFYVE27 Human)
    mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. mCerulean-hCOL1A1

    Plasmid

    #140112

    Purpose

    Expresses fluorescently-tagged human Type I procollagen α1 chain with mCerulean replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Insert

    Type I procollagen α1 (COL1A1 Human)
    

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    COL1A1 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pQTEV-PPP1R1A

    Plasmid

    #31331

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    protein phosphatase 1 (PPP1R1AP1 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pFP.R265

    Plasmid

    #138167

    Purpose

    Expresses the split mCherry reporter interrupted by the SspDnaB M86 artificially mini-intein (in cis)

    Depositor

    Baojun Wang

    Article

    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

    Inserts

    araC (araC )
    mCherryN(VDA).M86.(SDL)mCherryC.H6
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    P(araBAD) and iPC

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pQTEV-CIP29

    Plasmid

    #34822

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    cytokine induced protein 29 kDa (SARNP Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. FLAG-hLRP6-opt(20-1613)-9xHis_MESD_pFastBacDual

    Plasmid

    #216377

    Purpose

    Baculovirus transfer vector to co-express FLAG-LRP6-His (human sequence, codon-optimized for insect cell expression) and MESD chaperone (human sequence)

    Depositor

    William Weis

    Insert

    LDL receptor related protein 6 (LRP6 Human)
    

    Use

    Baculovirus

    Tags

    9xHis and HA signal sequence-FLAG-3C cleavage sit…

    Expression

    Insect

    Promoter

    Polyhedrin (LRP6)/p10 (MESD)

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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36. pCDFDuet-trAtCCD7-OsD27(pYL726 )

    Plasmid

    #178285

    Purpose

    Co-Expresses OsD27 and trAtCCD7 in E. coli. pCDFDuet-1 carrying D27 from Oryza sativa and N-terminus 31 amino-acid truncated CCD7 from Arabidopsis

    Depositor

    Yanran Li

    Article

    Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

    Inserts

    OsD27
    trAtCCD7
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. dABE

    Plasmid

    #124447

    Purpose

    Expresses dead Cas9 (dCas9)-ABE7.10

    Depositor

    George Church

    Article

    Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

    Insert

    dABE7.10
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Available Since

    April 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV-UbC-mCherry Rab5

    Plasmid

    #115239

    Purpose

    To express in mammalian cells the endocytic protein Rab5 fused to mCherry by transfection or by generating AAV virions.

    Depositor

    Pantelis Tsoulfas

    Insert

    Rab5a (RAB5A Homo sapiens, Human)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    Human UbC

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. lentiMutB3GNT5-blast

    Plasmid

    #208380

    Purpose

    lentiviral vector for expressing human B3GNT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

    Depositor

    Jennifer Kohler

    Insert

    Beta-1,3-N-Acetylglucosaminyltransferase 5 (B3GNT5 Human)
    

    Use

    Lentiviral

    Tags

    myc, FLAG

    Expression

    Mammalian

    Mutation

    nt 922 c->g (PAM site inactivation)

    Promoter

    EF-1 alpha core promoter

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pQTEV-PDAP1

    Plasmid

    #31586

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    PDGFA associated protein 1 (PDAP1 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge