We narrowed to 75,864 results for: Rest

1. pSBbi-Puro_LPAR_AKAR-WT

   Plasmid

   #125206

   Purpose

   SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based PKA activity reporter

   Depositor

   Eric Davis

   Insert

   LPAR_AKAR-WT
   

   Use

   Transposon

   Tags

   EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLS1/-11

   Plasmid

   #31492

   Depositor

   Kathleen Matthews

   Insert

   Lactose repressor with 11 amino acid C-terminal deletion (lacI E. coli variant)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 21, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pRL-TK 4x wt

   Plasmid

   #11313

   Depositor

   Phil Sharp

   Article

   Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

   Insert

   4 bulged binding sites for CXCR4 siRNA antisense
   

   Use

   Luciferase

   Tags

   Rr-luc

   Expression

   Mutation

   Promoter

   Available since

   Feb. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pLIX403-EYFP-ccdB-EBFP2

   Plasmid

   #158553

   Purpose

   Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPD706 DsRed-Express2-ZF13

   Plasmid

   #138793

   Purpose

   Constitutive expression of DsRed-Express2-ZF13 under the CMV promoter.

   Depositor

   Joshua Leonard

   Insert

   DsRed-Express2-ZF13
   

   Use

   Synthetic Biology

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLBS.EF-iRFP

   Plasmid

   #129329

   Purpose

   cytosol marker, lentivirus

   Depositor

   Yasushi Okada

   Article

   Yaginuma et al bioRxiv 2021.10.08.463131

   Insert

   iRFP (from Dr. M. Matsuda)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJK580

   Plasmid

   #71694

   Purpose

   Produces Acetobacter aceti 1023 thioredoxin with a C-terminal His6 tag (AaTrxAH6)

   Depositor

   T. J. Kappock

   Insert

   thioredoxin
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRS415-IVb

   Plasmid

   #31461

   Depositor

   Ana Kitazono

   Article

   Kitazono et al Gene. 2011 Sep 15;484(1-2):86-9. doi: 10

   Insert

   linker
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

9. Paxillin_PSCFP2_ mEOS2

   Plasmid

   #114192

   Purpose

   studying focal adhesion proteins by superresolution microscopy (PALM) with dual labelling

   Depositor

   Aleksandra Radenovic

   Insert

   Paxillin (PXN Chicken)
   

   Use

   Tags

   PS-CFP2 and mEos2

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMT643

    Plasmid

    #8610

    Depositor

    Robert Hartley

    Article

    Hartley et al Biochemistry 1993 Jun 15;32(23):5978-84.

    Insert

    barstar A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Two cysteines of barstar changed to alanine, C40A…

    Promoter

    Available since

    Jan. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. XE126 Rfz1myc CS2+

    Plasmid

    #16798

    Depositor

    Randall Moon

    Insert

    Rfz1 (Fzd1 Rat)
    

    Use

    Xenopus expression

    Tags

    myc

    Expression

    Mutation

    Promoter

    Available since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pCAGGS-S-P1-FLAG

    Plasmid

    #177095

    Purpose

    P1 variant S-protein with c-terminal FLAG tag

    Depositor

    Daniel Conway

    Insert

    S-protein P1 (S SARS-CoV-2)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pHXPL

    Plasmid

    #17667

    Depositor

    Cecilia Lo

    Insert

    Cx43 promoter (Gja1 Mouse)
    

    Use

    Tags

    LacZ

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. UBC45

    Plasmid

    #25469

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ubc45.001.200
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 23, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pENTRY4_ Pi21

    Plasmid

    #126904

    Purpose

    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.

    Depositor

    Sophien Kamoun

    Insert

    Wheat_live_Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBS3ndd

    Plasmid

    #97354

    Purpose

    Low background cloning vector for conventional or homologous recombination cloning with sticky blunt or TA compatible ends, kanamycin resistant

    Depositor

    Jamorn Somana

    Insert

    Nucleoid Disruptive Domain
    

    Use

    Cloning

    Tags

    Expression

    Bacterial

    Mutation

    changed methionine 1 to isoleucine

    Promoter

    E. coli lacZ promoter

    Available since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX-5X-1 SmB-N

    Plasmid

    #129075

    Purpose

    generation of GST-tagged N-terminal part of SmB protein using bacteria

    Depositor

    Takeshi Tsubata

    Insert

    SmB (SNRPB Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    contains amino acids 1-492

    Promoter

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HC080

    Plasmid

    #59545

    Purpose

    Expresses yeast Atg13 under the endogenous promoter with a GFP N-terminal tag

    Depositor

    Daniel Klionsky

    Insert

    ATG13 (ATG13 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    AvrII site created after start codon

    Promoter

    ATG13

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pCMV5 TBRI K to R

    Plasmid

    #19159

    Depositor

    Joan Massague

    Insert

    transforming growth factor, beta 1 (TGFBR1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K420R mutation

    Promoter

    Available since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pTLPflaG-small

    Plasmid

    #45476

    Depositor

    Michele Swanson

    Article

    Hammer et al Mol Microbiol. 1999 Aug;33(4):721-31.

    Insert

    flaA-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 4, 2013

    Availability

    Academic Institutions and Nonprofits only

21. pGPDluxAB(Ura)

    Plasmid

    #1119

    Depositor

    Susan Lindquist

    Article

    Singer et al Mol Cell 1998 Apr;1(5):639-48.

    Insert

    bacterial luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    n/a

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

22. pCMV6-XL4 FLAG-TM9SF4-Fc

    Plasmid

    #115770

    Purpose

    Expression of the extracellular domain of TM9SF4 with N-terminal FLAG tag and C-terminal hIgG tag.

    Depositor

    Davide Comoletti

    Insert

    TM9SF4 (TM9SF4 Human)
    

    Use

    Tags

    FLAG and hIgG/Fc

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PP-UEV1A

    Plasmid

    #25111

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PFC0255c:C34858
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

24. U6-Lin28aIR

    Plasmid

    #120530

    Purpose

    dsRNA expression

    Depositor

    Petr Svoboda

    Insert

    Lin28 (Lin28a Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pNC891

    Plasmid

    #20397

    Depositor

    Beverly Errede

    Article

    Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

    Insert

    YFP-reporter
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    X=M (see schematic)

    Promoter

    Available since

    March 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRK GFP Vinculin

    Plasmid

    #50531

    Depositor

    Kenneth Yamada

    Insert

    Vinculin (VCL Chicken)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

27. RS314

    Plasmid

    #17542

    Depositor

    Bert Vogelstein

    Article

    Thiagalingam et al Proc Natl Acad Sci U S A. 1995 Jun 20. 9

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

28. Abr in pAcG2T

    Plasmid

    #38162

    Depositor

    Nora Heisterkamp

    Insert

    ABR (ABR Human)
    

    Use

    Tags

    GST

    Expression

    Insect

    Mutation

    Promoter

    polyhedron

    Available since

    Aug. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

29. pRSII419

    Plasmid

    #35460

    Depositor

    Steven Haase

    Article

    Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

    Type

    Empty backbone

    Use

    Yeast centromere vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBac[6xP3-Tc-hsp-DsRedExpress-SV40]

    Plasmid

    #86448

    Purpose

    pBAc transposon with transformation marker for Tribolium transgenesis; Drives dsRedExpress (red fluorescent) expression in the eyes (much stronger than usual 3XP3); (Transformation plasmid)

    Depositor

    Gregor Bucher

    Insert

    6xP3-Tc-hsp-DsRedExpress-SV40
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. BcatMut-Tyr604

    Plasmid

    #24196

    Depositor

    David Rimm

    Insert

    beta-catenin (CTNNB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y604F

    Promoter

    Available since

    March 9, 2010

    Availability

    Academic Institutions and Nonprofits only

32. pCMV6-XL4 FLAG-TMEFF2-Fc

    Plasmid

    #115774

    Purpose

    Expression of the extracellular domain of TMEFF2 with N-terminal FLAG tag and C-terminal hIgG tag.

    Depositor

    Davide Comoletti

    Insert

    TMEFF2 (TMEFF2 Human)
    

    Use

    Tags

    FLAG and hIgG/Fc

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Oct. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. 830 pcDNA3 GFP PTEN 1-336

    Plasmid

    #10927

    Depositor

    William Sellers

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Amino acids 1-336

    Promoter

    Available since

    Jan. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

34. pTWEEN-miR-224

    Plasmid

    #35683

    Depositor

    George Calin

    Insert

    nsa-mir-224 (MIR224 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

35. pAc5.1B-EGFP-DmRBM7

    Plasmid

    #66594

    Purpose

    expresses EGFP-tagged RBM7 in Drosophila cells

    Depositor

    Elisa Izaurralde

    Insert

    RBM7 (CG11454 Fly)
    

    Use

    Drosophila expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Actin 5C

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRS316-IVa

    Plasmid

    #31456

    Depositor

    Ana Kitazono

    Article

    Kitazono et al Gene. 2011 Sep 15;484(1-2):86-9. doi: 10

    Insert

    linker
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

37. PLXNA2

    Plasmid

    #28279

    Depositor

    Cheryl Arrowsmith

    Insert

    PLXNA2 (PLXNA2 Human)
    

    Use

    Tags

    His with TEV cleavage

    Expression

    Bacterial

    Mutation

    residues 1490-1600.

    Promoter

    Available since

    Feb. 10, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

38. pGL3 CXCR4 1P

    Plasmid

    #11310

    Depositor

    Phil Sharp

    Article

    Doench et al Genes Dev. 2003 Feb 15. 17(4):438-42.

    Insert

    1 perfect binding site for CXCR4 siRNA antisense
    

    Use

    Luciferase

    Tags

    Pp-luc

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRSII329

    Plasmid

    #35473

    Depositor

    Steven Haase

    Article

    Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

    Type

    Empty backbone

    Use

    Yeast 2 micron vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3.1-hRIPK1-Flag-K599R

    Plasmid

    #112490

    Purpose

    Mammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.

    Depositor

    Junying Yuan

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K599R

    Promoter

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pRS413-IVa

    Plasmid

    #31458

    Depositor

    Ana Kitazono

    Article

    Kitazono et al Gene. 2011 Sep 15;484(1-2):86-9. doi: 10

    Insert

    linker
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

42. XE181 rat Frizzled-1-myc-pCD

    Plasmid

    #16819

    Depositor

    Randall Moon

    Insert

    frizzled-1 (Fzd1 Rat)
    

    Use

    Xenopus expression

    Tags

    myc

    Expression

    Mutation

    Promoter

    Available since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only

43. Rab5-L38R-mCherry

    Plasmid

    #102785

    Purpose

    Point mutation in Rab5 that reestablishes interaction with APPL1 when co-expressed with APPL1-N308D

    Depositor

    Donna Webb

    Insert

    Rab5a (RAB5A Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Leucine 38 changed to Arginine

    Promoter

    CMV

    Available since

    Jan. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. BcatMut-Tyr670

    Plasmid

    #24198

    Depositor

    David Rimm

    Insert

    beta-catenin (CTNNB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y670F

    Promoter

    Available since

    March 9, 2010

    Availability

    Academic Institutions and Nonprofits only

45. pBS cHoxA11 (CT#899)

    Plasmid

    #14007

    Depositor

    Cliff Tabin

    Insert

    HoxA11 (HOXA11 Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pX330 Mouse 5' Npm1 gRNA

    Plasmid

    #127900

    Purpose

    WT Cas9 Vector targeting the 5' end of the mouse Npm1 gene

    Depositor

    Mark Groudine

    Insert

    gRNA/Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pRS413-IVb

    Plasmid

    #31459

    Depositor

    Ana Kitazono

    Article

    Kitazono et al Gene. 2011 Sep 15;484(1-2):86-9. doi: 10

    Insert

    linker
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

48. Pf-GUK1

    Plasmid

    #25571

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    Pf-GUK1 (PfGK Plasmodium falciparum)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

49. HOXB4-WG-SP65

    Plasmid

    #20986

    Depositor

    Corey Largman

    Insert

    HOXB4 (HOXB4 Human)
    

    Use

    In vitro tnt

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

50. BcatMut-Tyr432

    Plasmid

    #24200

    Depositor

    David Rimm

    Insert

    beta-catenin (CTNNB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y432F

    Promoter

    Available since

    March 15, 2010

    Availability

    Academic Institutions and Nonprofits only