We narrowed to 5,817 results for: 129
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Plasmid#66329PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only
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pHAGE2-TetO-hSOX17
Plasmid#206389PurposeExpresses human SOX17 in mammalian cells, for lentivirus generation.DepositorInsertSOX17 (SOX17 Human)
UseLentiviralAvailable SinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
CHRM4-DuET
Plasmid#213210PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRCreb5gRNA
Plasmid#195021PurposeSequence specific sgRNA that guide Cas9 to the genomic region encoding the bovine Creb5 DNA binding domainDepositorAvailable SinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
ApoE3_mCh-SspB (pBS1144)
Plasmid#185326PurposeFor the mammalian expression of the human protein ApoE3 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formationDepositorInsertApoE3
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
mPapaya1-Farnesyl-5
Plasmid#56650PurposeLocalization: Membrane, Excitation: 530, Emission: 541DepositorInsertFarnesyl (HRAS Human)
TagsmPapaya1ExpressionMammalianMutationencodes final 20 aa of NM_001130442.1PromoterCMVAvailable SinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX Tyk2-SH2
Plasmid#46527DepositorInsertTyk2 (TYK2 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 452-567PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
TTMP-His-bio
Plasmid#52335PurposeExpresses full-length TPA-induced transmembrane protein ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.DepositorInsertTTMP (C3orf52 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot9_18-285_S
Plasmid#147480PurposeMammalian Expression of HsNot9_18-285DepositorInsertHsNot9_18-285 (CNOT9 Human)
ExpressionMammalianAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot9_18-285_S
Plasmid#147482PurposeMammalian Expression of HsNot9_18-285DepositorInsertHsNot9_18-285 (CNOT9 Human)
ExpressionMammalianAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
CD8a-hnRNP-M-EGFP
Plasmid#86054PurposeA mammalian expression plasmid encoding CD8a luminal and transmembrane domains followed by hnRNP-M (PY nuclear localization signal of hnRNP-M) and C-terminus EGFP.DepositorInsertCD8a (CD8A Human)
TagsGFPExpressionMammalianMutationCD8a luminal and transmembrane domians along with…PromoterCMVAvailable SinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgNotch2#2/Cre
Plasmid#193226PurposeLentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch2 geneDepositorAvailable SinceDec. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-MAPTau-N-10
Plasmid#55498PurposeLocalization: Microtubules, Excitation: 462, Emission: 492DepositorAvailable SinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSCV-TAP(FLAG-HA) CYLD
Plasmid#60031PurposeCYLD with N-terminal TAP-tag (HA,FLAG) and IRES-Puro to allow tagged protein to be maintainedDepositorInsertCylindromatosis (CYLD Human)
UseRetroviralTagsFLAG, HA, and IRES PuroExpressionMammalianPromoterLTR-driven expressionAvailable SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CRK-2/2-SV1/2
Plasmid#91319PurposeProtein expression and purification of human SH3 domain construct CRK-2/2-SV1/2DepositorInsertCRK-2/2-SV1/2 (CRK Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
DMGDH
Plasmid#155549PurposeFor use in RBP tethering screenDepositorAvailable SinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT5T_hp53_ST_CR2
Plasmid#34923DepositorInsertp53 DNA-binding (Res 94-358, deletion 293-321) (TP53 Human)
ExpressionBacterialMutationC135V, C141V, W146Y, C182S, V203A, R209P, C229Y, …PromoterT7Available SinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-TRR-C421-Y2404F
Plasmid#182846Purposemutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG inductionDepositorInsertTRR 2011-2431 (trr Fly)
ExpressionBacterialMutationmutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 20…Available SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-TYK2K930R-FF-VSV
Plasmid#139348PurposeExpression of kinase-dead TYK2-K930R missing the two tyrosines at the activation loopDepositorInsertTYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
TagsVSVExpressionMammalianMutationV362F-K930R-Y1054F-Y1055FPromoterCMVAvailable SinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-TYK21054-5FF-VSV
Plasmid#139347PurposeExpression of TYK2 protein mutated in the two tyrosines (Y1054F/Y1055F) of the activation loopDepositorInsertTYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
ExpressionMammalianMutationV362F-Y1054F-Y1055FPromoterCMVAvailable SinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only