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167,150 results

Showing: 7641 - 7680 of 167150 results

  1. pET-MBP-mSA2

    Plasmid
    #52319
    Purpose
    Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)
    Depositor
    Sheldon Park
    Article
    Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6
    Insert
    monomeric streptavidin 2
    Tags
    FLAG, His, MBP, and TEV
    Expression
    Bacterial
    Mutation
    S25H mutation compared to streptavidin
    Promoter
    T7
    Available Since
    July 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    52319-plasmid-map-sequence-id-237583

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  3. pDx_mScarlet3-H

    Plasmid
    #242970
    Purpose
    Dual expression vector for bacteria and mammalian cells producing mScarlet-3-H red fluorescent protein
    Depositor
    Dorus Gadella
    Insert
    mScarlet3-H
    Expression
    Bacterial and Mammalian
    Promoter
    CMV & Rha
    Available Since
    Nov. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    242970-plasmid-map-sequence-id-492366

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  7. SmBiT-JAK2 Vector

    Plasmid
    #238823
    Purpose
    Express SmBiT-JAK2 Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    JAK2 (JAK2 Human)
    Tags
    SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238823-plasmid-map-sequence-id-481977

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  8. NanoLuc-bRaf Vector

    Plasmid
    #238829
    Purpose
    Express NanoLuc-bRaf Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    BRAF (BRAF Human)
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238829-plasmid-map-sequence-id-479846

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  9. NanoBRET Assay Vector, NanoLuc-KRas(G12C)

    Plasmid
    #238582
    Purpose
    Express NanoLuc-KRas(G12C) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS (KRAS Human)
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Mutation
    G12C
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238582-plasmid-map-sequence-id-478460

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  10. pCMV-Frankenbody-HA-mScarlet3-tdP2A-Frankenbody-FLAG-mEGFP-IRESv4-HaloTag-tdMCP

    Plasmid
    #241140
    Purpose
    Expresses Anti-HA frankenbody-mScarlet3, anti-FLAG frankenbody-mEGFP, and HaloTag-tdMCP to track mature and nascent HA and FLAG-tagged proteins and MS2-tagged mRNAand MS2-tagged mRNA
    Depositor
    Tim Stasevich
    Article
    Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi
    Inserts
    Anti-FLAG frankenbody
    anti-FLAG frankenbody
    tdMCP
    Tags
    HaloTag, mEGFP, and mScarlet3
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    241140-plasmid-map-sequence-id-485256

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  11. SmBiT-PIK3R1 Fusion Vector

    Plasmid
    #237095
    Purpose
    Express SmBiT-PIK3R1 Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    PIK3R1 (PIK3R1 Human)
    Tags
    SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237095-plasmid-map-sequence-id-473324

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  12. PIK3R1-SmBiT Fusion Vector

    Plasmid
    #237097
    Purpose
    Express PIK3R1-SmBiT Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    PIK3R1 (PIK3R1 Human)
    Tags
    SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237097-plasmid-map-sequence-id-473326

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  13. NanoBRET Assay Vector, NanoLuc-PIK3CA

    Plasmid
    #236855
    Purpose
    Express NanoLuc(R)-PIK3CA in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    PIK3CA (PIK3CA Human)
    Tags
    HaloTag (R) and NanoLuc (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236855-plasmid-map-sequence-id-472446

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  14. pIL6-PMEL-hbb

    Plasmid
    #239868
    Purpose
    Template for in vitro transcription of PMEL (gp100), with signal peptide replaced by the IL6 signal peptide, flanked by the human haemoglobin beta 5' and 3' UTRs.
    Depositor
    Catherine Jopling
    Article
    Smart et al Mol Ther Nucleic Acids. 2025 Jun 24;36(3
    Insert
    Full premelanosome coding sequence with IL6 signal peptide, flanked by human haemoglobin beta 5' and 3' UTRs (PMEL Human)
    Expression
    Mammalian
    Mutation
    Valine to Glycine change is at amino acid 26 of n…
    Promoter
    T7 (included as part of insert)
    Available Since
    July 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    239868-plasmid-map-sequence-id-477826

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  18. pXPR_068

    Plasmid
    #164555
    Purpose
    Lentiviral vector that enables Flp-mediated sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s
    Insert
    sgRNA cassette
    Use
    CRISPR and Lentiviral; Flp/frt
    Expression
    Mammalian
    Promoter
    Human U6
    Available Since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    164555-plasmid-map-sequence-id-330129

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  19. TFORF1529

    Plasmid
    #143820
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF605 (ZNF605 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143820-plasmid-map-sequence-id-396256

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  21. TFORF1239

    Plasmid
    #143775
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ZNF431 (ZNF431 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    143775-plasmid-map-sequence-id-338763

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  23. pcDNA5/FRT/TO-GAlphaz-RLuc8

    Plasmid
    #140978
    Purpose
    Encodes a G alpha subunit (GNAZ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphaz-Rluc8 (GNAZ Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140978-plasmid-map-sequence-id-300661

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  25. BRD4

    Plasmid
    #38942
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BRD4 (BRD4 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    38942-plasmid-map-sequence-id-348518

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  29. pEC3115 (pSpy0K6-P23_mKate2~*ssrA)

    Plasmid
    #218509
    Purpose
    integrative plasmid enabling constitutive expression of mKate2 in Streptococcus pyogenes, integrates into the transcriptionally silent locus Spy_1078 via homologous recombination
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    mKate2
    Tags
    ssrA degradation tag
    Expression
    Bacterial
    Mutation
    Deletion of Plac_mrfp cassette, introduction of a…
    Promoter
    P23
    Available Since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    218509-plasmid-map-sequence-id-438730

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  32. pCV020-tetQ

    Plasmid
    #232826
    Purpose
    Random transposon mutagenesis vector for Bacteroidales species - tetracycline
    Depositor
    Athanasios Typas
    Insert
    tetQ
    Expression
    Bacterial
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232826-plasmid-map-sequence-id-462958

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  34. Lamin A-mRFP

    Plasmid
    #124268
    Purpose
    Wild-type Lamin A, C-terminally tagged with mRFP
    Depositor
    Eric Schirmer
    Insert
    LMNA (LMNA Human)
    Tags
    mRFP
    Expression
    Mammalian
    Available Since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124268-plasmid-map-sequence-id-241940

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  36. pCMV-T7-ABE8e-SpCas9-NRTH-P2A-EGFP (RMD21)

    Plasmid
    #197503
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRTH-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-NRTH(D10A/I32…
    Promoter
    CMV and T7
    Available Since
    March 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197503-plasmid-map-sequence-id-386604

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  38. pET His6 MBP TEV HDAC8_374

    Plasmid
    #122174
    Purpose
    Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag
    Depositor
    David Christianson
    Article
    Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-22
    Insert
    His6 MBP TEV HDAC8_374 (HDAC8 Human)
    Tags
    hexahistidine tag and tobacco etch virus protease…
    Expression
    Bacterial
    Mutation
    Residues Met1–Pro7 and H375-V377 have been delete…
    Promoter
    T7
    Available Since
    March 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    122174-plasmid-map-sequence-id-237469

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Showing: 7641 - 7680 of 167150 results