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Addgene

We narrowed to 4,886 results for: phy

Showing: 3821 - 3840 of 4886 results
  1. pENTR-DMD-Donor

    Plasmid
    #60605
    Purpose
    Knock-in donor vector to insert Exon 44 in front of Exon 45 of human DYSTROPHIN (DMD)gene, include EF1a-hygromycin resistance cassette flanked by loxP sequences.
    Depositor
    Insert
    Hygromycin resistant gene
    Use
    Knock-in donor vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
  2. pCC_02 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-Cas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139087
    Purpose
    Expresses human codon-optimized Cas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Insert
    SpCas9-NG
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    L1111R, D1135V,G1218R, E1219F, A1322R, R1335V, T1…
    Promoter
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. pEGB3 Pnos:Luciferase:Tnos-SF-35S:Renilla:Tnos-35S:P19:Tnos-SF (GB1116)

    Plasmid
    #68218
    Purpose
    Module composed of 3 transcriptional units: Luciferase and Renilla proteins, and the Tomato bushy stunt virus (TBSV) silencing suppressor P19 plant expression
    Depositor
    Insert
    Luc / Renilla / P19
    Use
    Luciferase and Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Pnos / 35S / 35S
    Available since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only
  4. pAF197

    Plasmid
    #135930
    Purpose
    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil CTLA4-Fc-mCherry fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. CTLA4).
    Depositor
    Insert
    CTLA4 fused to IgG Fc and to mCherry
    Use
    Transposon
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
  5. pBItetDT240GFP

    Plasmid
    #96904
    Purpose
    tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 240 interrupted CTG repeats in exon 15 located
    Depositor
    Insert
    EGFP and human DMPK exons 11-15 with 240 interrupted CTG repeats (DMPK Human)
    Use
    Tetracycline responsive and bidirectional
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline responsive and bidirectional
    Available since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
  6. CiDr-VSP L223F/C302S-mCherry pcDNA3.1 (eVSP CSmutant)

    Plasmid
    #140893
    Purpose
    Expresses modified Danio rerio VSP without phosphatase activity (inactive mutant of enhanced VSP) fused with mCherry in mammalian cel
    Depositor
    Insert
    CiDr-VSP L223F/C302S-mCherry (vsp, tpte Ciona intestinalis, Zebrafish)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    changed 223L to F in Dr-VSP (zebrafish TPTE), cha…
    Promoter
    Available since
    May 13, 2020
    Availability
    Academic Institutions and Nonprofits only
  7. pAAV-CreLite

    Plasmid
    #131785
    Purpose
    AAV donor/transfer vector expressing CreLite system components, PhyBΔCreC and PIF6CreN, driven by CBh promoter
    Depositor
    Insert
    PhyBCreC-P2A-PIF6CreN
    Use
    AAV, Cre/Lox, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 3821 - 3840 of 4886 results