We narrowed to 3,219 results for: cat.3

1. gCH198 (crCD55-4_crB2M-1_crB2M-3_crCLTA-4_crFOLH1-1_crCD151-3_crHBG-3_crKIT-2_crKIT-3_crCD81-1)

   Plasmid

   #217345

   Purpose

   crRNA array targeting CD55, B2M, CLTA, FOLH1, CD151, HBG1/HBG2, KIT, CD81

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Inserts

   crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
   crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
   crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
   crCLTA-4 gRNA: ggctctgcaacaccgcctagacc (CLTA Human)
   crFOLH1-1 gRNA: gctccagacctggggtccagttt (FOLH1 Human)
   crCD151-3 gRNA: cgggaggccgcacccaccgcctg (CD151 Human)
   crHBG-3 gRNA: ttcttcatccctagccagccgcc (HBG1, HBG2 Human)
   crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
   crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
   crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)
   

   Use

   CRISPR and Lentiviral

   Promoter

   hU6

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pORANGE Gria3-GFP KI

   Plasmid

   #131491

   Purpose

   Endogenous tagging of GluA3: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Gria3, Gria3 Rat, Mouse)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. BCR/ABL P190 transgenic construct

   Plasmid

   #38185

   Depositor

   Nora Heisterkamp

   Article

   Heisterkamp et al Nature. 1990 Mar 15;344(6263):251-3.

   Insert

   BCR/ABL P190 (ABL1, BCR Human)
   

   Use

   Construct for making transgenic mice

   Promoter

   truncated mouse MT promoter

   Available Since

   Oct. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOPINB-hRNF216(457-784)

   Plasmid

   #171922

   Purpose

   Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic helix-RBR-helix construct suitable for activity assays.

   Depositor

   Bernhard Lechtenberg

   Article

   Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d

   Insert

   E3 ubiquitin-protein ligase RNF216 (RNF216 Human)
   

   Tags

   3C protease cleavage site and 6x-His tag

   Expression

   Bacterial

   Mutation

   codon optimised for expression in E. coli

   Promoter

   T7

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKK-TEV-ProteinA

   Plasmid

   #105788

   Purpose

   Expression of your protein of interest in fusion with two IgG binding domains of Staphylococcus aureus protein A at the C-terminus (cleavable by TEV) (PMID: 3507693, 10504710). Protein purification.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-ProteinA

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKK-MBP-TEV

   Plasmid

   #105771

   Purpose

   Expression of your protein of interest in fusion with MBP at the N-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   MBP-TEV

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKK-TEV-MBP

   Plasmid

   #105787

   Purpose

   Expression of your protein of interest in fusion with MBP at the C-terminus (cleavable by TEV). The maltose binding protein tag allows convenient purification of proteins (PMID: 22442034, 19935684).

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   TEV-MBP

   Expression

   Mammalian

   Available Since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cntn3-AP-His

   Plasmid

   #71941

   Purpose

   Expresses the extracellular region of the Contactin 3 protein (truncated immediately upstream of propeptide cleavage and GPI-attachement site), C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Cntn3 (Cntn3 Mouse)
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMK188

   Plasmid

   #226659

   Purpose

   pCB2.4 - pBR322 shuttle vector that replicates in E. coli and Synechococcus sp. PCC 7002, contains HIP1 site

   Depositor

   David Nielsen

   Type

   Empty backbone

   Use

   Unspecified

   Available Since

   Jan. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMK191

    Plasmid

    #226658

    Purpose

    Broad host range WVO1 vector that replicates in Synechococcus sp. PCC 7002, GentR, contains HIP1 site

    Depositor

    David Nielsen

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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