We narrowed to 11,662 results for: cel.2

1. pAAV-CAGGS-Flex/3'USS(103)-GFP

   Plasmid

   #197890

   Purpose

   Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   CAG

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CAGGS-Flex/3'USS(553)-GFP

   Plasmid

   #197888

   Purpose

   Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   CAG

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-CAGGS-Flex/3'USS(285)-GFP

   Plasmid

   #197889

   Purpose

   Can be used to generate AAV virus that will express GFP in the presence of Cre including the shortage of a unilateral spacer sequence(USS)

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   CAG

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSFDuet-1_mNeongreen-Erythropoietin

   Plasmid

   #182422

   Purpose

   Bacterial expression of mNeongreen-Erythropoietin fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   mNeongreen-eythropoietin (EPO Human)
   

   Use

   Tags

   mNeongreen

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. KTK_139

   Plasmid

   #180565

   Purpose

   Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2

   Depositor

   Tom Ellis

   Article

   Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

   Insert

   Spacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-EngPDm

   Plasmid

   #64040

   Purpose

   Mammalian expression of mutant paired domain of Pax8 fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)

   Depositor

   Daniel Christophe

   Article

   Christophe-Hobertus et al Mol Cell Endocrinol. 2007 Jan 29;264(1-2

   Insert

   Pax8 paired domain (Pax8 Mouse)
   

   Use

   Tags

   Eng (N-terminal repressor domain of the drosophi…

   Expression

   Mammalian

   Mutation

   paired domain, aa 1-147, with aa 30 to 32 changed…

   Promoter

   CMV

   Available since

   April 23, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-EngHDm

   Plasmid

   #64038

   Purpose

   Mammalian expression of TTF-1 mutant homeodomain fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)

   Depositor

   Daniel Christophe

   Article

   Christophe-Hobertus et al Mol Cell Endocrinol. 2007 Jan 29;264(1-2

   Insert

   TTF-1 (TTF1 dog)
   

   Use

   Tags

   Eng (N-terminal repressor domain of the drosophi…

   Expression

   Mammalian

   Mutation

   homeodomain, aa 154-226, with aa 48–50 replaced b…

   Promoter

   CMV

   Available since

   April 23, 2015

   Availability

   Academic Institutions and Nonprofits only

8. XE159 GFP-delta LZ-XDpr-delta PDZB-CS2+

   Plasmid

   #16763

   Depositor

   Randall Moon

   Article

   Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

   Insert

   delta LZ-XDpr-delta PDZB (dact1-b Frog)
   

   Use

   Xenopus expression

   Tags

   gfp

   Expression

   Mutation

   XDpr that is doubly truncated to delete the first…

   Promoter

   Available since

   March 13, 2008

   Availability

   Academic Institutions and Nonprofits only

9. mCherry-FKBP-TPD54(L53P,L67P)

   Plasmid

   #172458

   Purpose

   Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry-FKBP tag L53P,L67P mutant

   Depositor

   Stephen Royle

   Article

   Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

   Insert

   TPD54 (TPD52L2 Human)
   

   Use

   Tags

   mCherry-FKBP

   Expression

   Mammalian

   Mutation

   changed Leucines 53 and 67 to proline (breaks coi…

   Promoter

   CMV

   Available since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLIK-IDH1-FLAG

    Plasmid

    #66802

    Purpose

    Tet-inducible expression of IDH1 in mammalian cells, 3rd generation lentiviral vector

    Depositor

    Christian Metallo

    Article

    Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

    Insert

    IDH1 (IDH1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-UBC-FLEX-XRI-FLAG

    Plasmid

    #178057

    Purpose

    Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with FLAG tag, under UBC promoter with a FLEX switch.

    Depositor

    Edward Boyden

    Article

    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    XRI-FLAG
    

    Use

    AAV

    Tags

    FLAG-MBP

    Expression

    Mammalian

    Mutation

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Oct. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFSynWSyt1C2AB(80-421)msGFP

    Plasmid

    #209014

    Purpose

    Overexpression of truncated syt1 tagged with GFP in mammalian cells

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    aa 80-421 only

    Promoter

    Available since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQC.Flip-nluc(LgBiT1-8)CP[TEV]

    Plasmid

    #200122

    Purpose

    Retroviral expression of Flip-nluc(LgBiT1-8)CP[TEV] protease reporter and T2A mCherry in mammalian cells

    Depositor

    Eiji Morita

    Article

    Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

    Insert

    Flip-nluc(LgBiT1-8)CP[TEV] T2A mCherry
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. FLAG-TKGAPDH in pLJM60

    Plasmid

    #154217

    Purpose

    Lentiviral vector for stable expression of FLAG-TK-GAPDH

    Depositor

    David Sabatini

    Article

    Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

    Insert

    GAPDH
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    cDNA has been codon optimized for human cell expr…

    Promoter

    CMV

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSCL.139

    Plasmid

    #184994

    Purpose

    Express Cas9-P2A-Eco1RT in human cells

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    SpCas9-P2A-Eco1RT
    

    Use

    Tags

    SV40NLS

    Expression

    Mammalian

    Mutation

    Human codon optimized RT

    Promoter

    TetOn-3g

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. CaM/pIRES2-eGFP

    Plasmid

    #111499

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    calmodulin [Xenopus laevis] (calm2 Frog)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. hOC-GFPtpz

    Plasmid

    #110207

    Purpose

    Expresses GFP in mature bone cells.

    Depositor

    David Rowe

    Article

    Bilic-Curcic et al Genesis. 2005 Oct;43(2):87-98. doi: 10.1

    Insert

    eGFPtopaz
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    Human osteocalcin (BGLAP)

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. YFP-HA-DAT

    Plasmid

    #90244

    Purpose

    Expresses YFP-HA-DAT in mammalian cells

    Depositor

    Alexander Sorkin

    Article

    Sorkina et al J Neurosci. 2006 Aug 2;26(31):8195-205.

    Insert

    DAT (dopamine transporter) (SLC6A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    residues 193–203 of human DAT were replaced in YF…

    Promoter

    CMV

    Available since

    June 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFSynWSyt1C2AB(143-421)msGFP

    Plasmid

    #209015

    Purpose

    Overexpression of truncated syt1 tagged with GFP in mammalian cells

    Depositor

    Edwin Chapman

    Article

    Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

    Insert

    syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    aa 143-421 only

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. H5_fold-0_Elsa

    Plasmid

    #201825

    Purpose

    Expresses H5_fold-0_Elsa in bacterial cells

    Depositor

    Nobuyasu Koga

    Article

    Sakuma et al Nat Struct Mol Biol. 2024 Feb;31(2):275-

    Insert

    H5_fold-0_Elsa
    

    Use

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBGSA FPR1 H4 (haplotype 4)

    Plasmid

    #66718

    Purpose

    Constitutive expression of FPR1 in mammalian cells

    Depositor

    Heini Miettinen

    Article

    Gripentrog et al Immunogenetics. 2008 Feb;60(2):83-93. do

    Insert

    FPR1 (haplotype 4) (FPR1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SR-alpha

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pSLIK-IDH1-R132H-FLAG

    Plasmid

    #66803

    Purpose

    Tet-inducible expression of mutant IDH1 in mammalian cells, 3rd generation lentiviral vector

    Depositor

    Christian Metallo

    Article

    Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

    Insert

    IDH1 (IDH1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R132H

    Promoter

    CMV

    Available since

    Feb. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA5/FRT/TO HIS HSPH2

    Plasmid

    #19535

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

    Insert

    heat shock 70kDa protein 4 (HSPA4 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    mutations A381T, T732A, and an insertion of "…

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. P2_HNF4A8

    Plasmid

    #31094

    Depositor

    Gerhart Ryffel

    Article

    Senkel et al BMC Res Notes. 2009 . 2():210.

    Insert

    HNF4A (HNF4A Human)
    

    Use

    Flp/frt

    Tags

    myc

    Expression

    Mammalian

    Mutation

    splice variant 8 derived from promoter P2

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CIP2Aprom1802bp-pGL4.10Luc

    Plasmid

    #60868

    Purpose

    Luc reporter driven by 1802 bp of the CIP2A promoter

    Depositor

    Jukka Westermarck

    Article

    Khanna et al PLoS One. 2011 Mar 22;6(3):e17979. doi:

    Insert

    1802 bp promoter fragment of Cancerous Inhibitor of PP2A (CIP2A Human)
    

    Use

    Luciferase; Promoterless

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CMV-138_HNF4A8

    Plasmid

    #31093

    Depositor

    Gerhart Ryffel

    Article

    Senkel et al BMC Res Notes. 2009 . 2():210.

    Insert

    HNF4A (HNF4A Human)
    

    Use

    Flp/frt

    Tags

    myc

    Expression

    Mammalian

    Mutation

    splice variant 8 of HNF4A, deletion of CMV promo…

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. nes374tk/lacZ

    Plasmid

    #47615

    Purpose

    used to create transgenic mice expressing LacZ reporter with Nestin enhancer

    Depositor

    Urban Lendahl

    Article

    Lothian et al Exp Cell Res. 1999 May 1;248(2):509-19.

    Insert

    nestin 2nd intron fragment (NES Human)
    

    Use

    Enhancer reporter

    Tags

    HSV tk promoter and lacZ

    Expression

    Mutation

    374 bp from 2nd intron (comprising bp 1153 to 152…

    Promoter

    HSV tk promoter

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

28. CIP2Aprom285bp-pGL4.10Luc

    Plasmid

    #60873

    Purpose

    Luc reporter driven by 285 bp of the CIP2A promoter

    Depositor

    Jukka Westermarck

    Article

    Khanna et al PLoS One. 2011 Mar 22;6(3):e17979. doi:

    Insert

    285 bp promoter fragment of Cancerous Inhibitor of PP2A2 (CIP2A Human)
    

    Use

    Luciferase; Promoterless

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 5, 2016

    Availability

    Academic Institutions and Nonprofits only

29. P2_DD-HNF4A8

    Plasmid

    #31095

    Depositor

    Gerhart Ryffel

    Article

    Senkel et al BMC Res Notes. 2009 . 2():210.

    Insert

    HNF4A (HNF4A Human)
    

    Use

    Flp/frt

    Tags

    FKBP L106P and myc

    Expression

    Mammalian

    Mutation

    splice variant 8 derived from promoter P2

    Promoter

    Available since

    Oct. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pROS13-Tps2/Gsy1

    Plasmid

    #196613

    Purpose

    Encoding guide RNAs for the knock out of TPS2 and GSY1 genes

    Depositor

    Matthias Heinemann

    Article

    Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

    Insert

    S000001911 (TPS2, GSY1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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