We narrowed to 18,263 results for: Met;

1. pB-CAG-FLEX-F14F15-BFP-(mCherry)'

   Plasmid

   #201831

   Purpose

   piggyBac transposon plasmid for making Cre and Flpo reporter cell lines using piggyBac transposase

   Depositor

   Ian Wickersham

   Article

   Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Inserts

   CAG
   BFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. FpG5

   Plasmid

   #69443

   Purpose

   lentiviral GFP reporter plasmid for high throughput functional detection of active transcriptional regulatory DNAs

   Depositor

   Lisa Dailey

   Article

   Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

   Inserts

   minimal FGF4 promoter
   EGFP
   Ubiquitin promoter
   Hygromycin resistance
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-mNeptune2

   Plasmid

   #51309

   Purpose

   Expresses far-red fluorescent protein mNeptune2 in mammalian cells

   Depositor

   Michael Lin

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   mNeptune2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCyRFP1-RhoA

   Plasmid

   #84358

   Purpose

   fusion protein of RhoA, FRET donor

   Depositor

   Ryohei Yasuda

   Article

   Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   mCyRFP1-RhoA (RHOA Synthetic, Human)
   

   Use

   Tags

   mCyRFP1 N terminal fusion to RhoA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. APLF-c001

   Plasmid

   #173077

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   APLF (APLF Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   K360-V448, codon optimized

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTag-RFP-C-h-Rab7L1-c-Myc

   Plasmid

   #79804

   Purpose

   Express TagRFP labeled human Rab7L1.

   Depositor

   James Johnson

   Article

   Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

   Insert

   Rab7L1 (RAB29 Human)
   

   Use

   Tags

   TagRFP and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. 5HT6-YC3.60

   Plasmid

   #48339

   Purpose

   FRET reporter for Ca2+ inside primary cilia

   Depositor

   Takanari Inoue

   Article

   Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

   Insert

   5-hydroxytriptamine receptor isoform 6 (Htr6 Mouse)
   

   Use

   Tags

   YC3.60

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pDL14

   Plasmid

   #106901

   Purpose

   pDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent manner

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

   Inserts

   gam
   bet
   mutS
   lacI
   rhaR
   rhaS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Lysine 622 was replaced by Alanine

   Promoter

   Available since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-LbCpf1-Blast

   Plasmid

   #84751

   Purpose

   Expresses human codon-optimized LbCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

   Insert

   LbCpf1
   

   Use

   CRISPR and Lentiviral

   Tags

   NLS-3xHA (C terminal on insert)

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. mTagRFP-T-Clathrin-15

    Plasmid

    #58005

    Purpose

    Localization: Clathrin Vesicles, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Clathrin (CLTB Human)
    

    Use

    Tags

    mTagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pPB12A

    Plasmid

    #210035

    Purpose

    Contains cloning site for Cas12a gRNA cassette, inducible Cas12a, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas12a
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)

    Plasmid

    #47550

    Purpose

    Cas9 + sgRNA plasmid that is targeted to a genomic site near the ttTi5605 Mos1 insertion allele

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

    Inserts

    Cas9
    ttTi5605 sgRNA
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Worm

    Mutation

    Codon optimized and with synthetic introns for C.…

    Promoter

    U6 and eef-1A.1 (eft-3)

    Available since

    Sept. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti_CamKIIa_mKate2

    Plasmid

    #96942

    Purpose

    To label mature excitatory iPSC-derived human neurons

    Depositor

    Oleksandr Shcheglovitov, Ricardo Dolmetsch

    Article

    Shcheglovitov et al Nature. 2013 Nov 14;503(7475):267-71. do

    Insert

    far-red fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    E87D (please see depositor comments below)

    Promoter

    CaMKIIa

    Available since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXS-IRES-Blast coSHMT2

    Plasmid

    #106299

    Purpose

    Expresses SHMT2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    serine hydroxymethyltransferase 2 (SHMT2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Codon optimized

    Promoter

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKG-GW1

    Plasmid

    #203163

    Purpose

    Yeast expression vector; To express proteins under control of TDH3 promoter and terminator using leucine selection

    Depositor

    William Sessa

    Article

    Park et al Cell Metab. 2014 Sep 2;20(3):448-57. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. mTagRFP-T-Cx26-7

    Plasmid

    #58007

    Purpose

    Localization: Gap Junctions, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Insert

    Cx26 (Gjb2 Rat)
    

    Use

    Tags

    mTagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pTag-RFP-C-h-Rab5a-c-Myc

    Plasmid

    #79802

    Purpose

    Express TagRFP labeled human Rab5a (GTPase located on early endosomal membranes).

    Depositor

    James Johnson

    Article

    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

    Insert

    Rab5a (RAB5A Human)
    

    Use

    Tags

    TagRFP and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pECE M2-SNX17 WT

    Plasmid

    #69811

    Purpose

    Expresses M2-SNX17 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    Sorting nexin-17 (SNX17 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 early promoter

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBV65

    Plasmid

    #108363

    Purpose

    A nourseothricin resistance marker used to construct gene disruptions in Candida glabrata. The natMX6 marker can be evicted by growth on media lacking methionine and containing estradiol.

    Depositor

    Scott Moye-Rowley

    Article

    Vu et al mSphere. 2018 Mar 28;3(2). pii: mSphere0

    Insert

    loxP-natMX6-CgMET3prom-cre-loxP
    

    Use

    Gene disruption marker for candida glabrata

    Tags

    Expression

    Mutation

    Promoter

    C. glabrata MET3

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. AB.pCCL.sin.cPPT.U6.miR-17-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46594

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-17 Decoy (MIR17, Mir17 Mouse, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only